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MedKoo Cat#: 596878 | Name: Troponin I (104-115)

Description:

WARNING: This product is for research use only, not for human or veterinary use.

Troponin I (104-115) is a cardiac and skeletal muscle protein useful in the laboratory diagnosis of heart attack. It occurs in different plasma concentration but the same circumstances as troponin T - either test can be performed for confirmation of cardiac muscle damage and laboratories usually offer one test or the other. Troponin I is a part of the troponin protein complex, where it binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place. Because of it, myosin cannot bind actin in relaxed muscle. When calcium binds to the troponin C it causes conformational changes which lead to dislocation of troponin I and finally tropomyosin leaves the binding site for myosin on actin leading to contraction of muscle. The letter I is given due to its inhibitory character.

Chemical Structure

Troponin I (104-115)
Troponin I (104-115)
CAS#98353-69-6

Theoretical Analysis

MedKoo Cat#: 596878

Name: Troponin I (104-115)

CAS#: 98353-69-6

Chemical Formula: C70H123N27O13

Exact Mass: 1549.9794

Molecular Weight: 1550.93

Elemental Analysis: C, 54.21; H, 7.99; N, 24.38; O, 13.41

Price and Availability

This product is currently not in stock but may be available through custom synthesis. To ensure cost efficiency, the minimum order quantity is 1 gram. The estimated lead time is 2 to 4 months, with pricing dependent on the complexity of the synthesis (typically high for intricate chemistries). Quotes for quantities below 1 gram will not be provided. To request a quote, please click the button below. Note: If this product becomes available in stock in the future, pricing will be listed accordingly.
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Related CAS #
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Synonym
Troponin I (104-115); Tni peptide (104-115); Ac-Gly-lys-phe-lys-arg-pro-pro-leu-arg-arg-val-arg-NH2;
IUPAC/Chemical Name
(S)-1-(acetylglycyl-L-lysyl-L-phenylalanyl-L-lysyl-L-arginyl-L-prolyl)-N-((S)-1-(((S)-1-(((S)-1-(((S)-1-(((S)-1-amino-5-guanidino-1-oxopentan-2-yl)amino)-3-methyl-1-oxobutan-2-yl)amino)-5-guanidino-1-oxopentan-2-yl)amino)-5-guanidino-1-oxopentan-2-yl)amino)-4-methyl-1-oxopentan-2-yl)pyrrolidine-2-carboxamide
InChi Key
RIGIYQFULOLFNC-DNCXWKQBSA-N
InChi Code
InChI=1S/C70H123N27O13/c1-40(2)37-50(61(105)91-47(24-14-32-83-68(76)77)58(102)89-48(25-15-33-84-69(78)79)60(104)95-55(41(3)4)64(108)88-44(56(73)100)23-13-31-82-67(74)75)94-63(107)52-27-17-35-96(52)66(110)53-28-18-36-97(53)65(109)49(26-16-34-85-70(80)81)92-59(103)46(22-10-12-30-72)90-62(106)51(38-43-19-7-6-8-20-43)93-57(101)45(21-9-11-29-71)87-54(99)39-86-42(5)98/h6-8,19-20,40-41,44-53,55H,9-18,21-39,71-72H2,1-5H3,(H2,73,100)(H,86,98)(H,87,99)(H,88,108)(H,89,102)(H,90,106)(H,91,105)(H,92,103)(H,93,101)(H,94,107)(H,95,104)(H4,74,75,82)(H4,76,77,83)(H4,78,79,84)(H4,80,81,85)/t44-,45-,46-,47-,48-,49-,50-,51-,52-,53-,55-/m0/s1
SMILES Code
N=C(N)NCCC[C@@H](C(N)=O)NC([C@H](C(C)C)NC([C@H](CCCNC(N)=N)NC([C@H](CCCNC(N)=N)NC([C@H](CC(C)C)NC([C@H]1N(C([C@H]2N(C([C@H](CCCNC(N)=N)NC([C@H](CCCCN)NC([C@H](CC3=CC=CC=C3)NC([C@H](CCCCN)NC(CNC(C)=O)=O)=O)=O)=O)=O)CCC2)=O)CCC1)=O)=O)=O)=O)=O
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info

Preparing Stock Solutions

The following data is based on the product molecular weight 1,550.93 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1: Schachat F, Brandt PW. The troponin I: inhibitory peptide uncouples force generation and the cooperativity of contractile activation in mammalian skeletal muscle. J Muscle Res Cell Motil. 2013 May;34(2):83-92. doi: 10.1007/s10974-013-9336-y. Epub 2013 Jan 23. PubMed PMID: 23340900. 2: Busche MN, Pavlov V, Takahashi K, Stahl GL. Myocardial ischemia and reperfusion injury is dependent on both IgM and mannose-binding lectin. Am J Physiol Heart Circ Physiol. 2009 Nov;297(5):H1853-9. doi: 10.1152/ajpheart.00049.2009. Epub 2009 Sep 11. PubMed PMID: 19749170; PubMed Central PMCID: PMC2781373. 3: Tanaka H, Takeya Y, Doi T, Yumoto F, Tanokura M, Ohtsuki I, Nishita K, Ojima T. Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I. FEBS J. 2005 Sep;272(17):4475-86. PubMed PMID: 16128816. 4: Ngai SM, Pearlstone JR, Farah CS, Reinach FC, Smillie LB, Hodges RS. Structural and functional studies on Troponin I and Troponin C interactions. J Cell Biochem. 2001 Jun 26-Jul 25;83(1):33-46. Erratum in: J Cell Biochem 2002;84(4):847. PubMed PMID: 11500952. 5: Petzold T, Feindt P, Sunderdiek U, Boeken U, Fischer Y, Gams E. Heart-type fatty acid binding protein (hFABP) in the diagnosis of myocardial damage in coronary artery bypass grafting. Eur J Cardiothorac Surg. 2001 Jun;19(6):859-64. PubMed PMID: 11404143. 6: Morjana N, Clark D, Tal R. Biochemical and immunological properties of human cardiac troponin I fragments. Biotechnol Appl Biochem. 2001 Apr;33(Pt 2):107-15. PubMed PMID: 11277863. 7: Van Eyk JE, Thomas LT, Tripet B, Wiesner RJ, Pearlstone JR, Farah CS, Reinach FC, Hodges RS. Distinct regions of troponin I regulate Ca2+-dependent activation and Ca2+ sensitivity of the acto-S1-TM ATPase activity of the thin filament. J Biol Chem. 1997 Apr 18;272(16):10529-37. PubMed PMID: 9099697. 8: Kobayashi T, Leavis PC, Collins JH. Interaction of a troponin I inhibitory peptide with both domains of troponin C. Biochim Biophys Acta. 1996 May 2;1294(1):25-30. PubMed PMID: 8639710. 9: Van Eyk JE, Caday-Malcolm RA, Yu L, Irvin RT, Hodges RS. Anti-peptide monoclonal antibody imaging of a common binding domain involved in muscle regulation. Protein Sci. 1995 Apr;4(4):781-90. PubMed PMID: 7613476; PubMed Central PMCID: PMC2143110. 10: Chandra M, McCubbin WD, Oikawa K, Kay CM, Smillie LB. Ca2+, Mg2+, and troponin I inhibitory peptide binding to a Phe-154 to Trp mutant of chicken skeletal muscle troponin C. Biochemistry. 1994 Mar 15;33(10):2961-9. PubMed PMID: 8130210. 11: Ngai SM, Sönnichsen FD, Hodges RS. Photochemical cross-linking between native rabbit skeletal troponin C and benzoylbenzoyl-troponin I inhibitory peptide, residues 104-115. J Biol Chem. 1994 Jan 21;269(3):2165-72. PubMed PMID: 8294472. 12: Slupsky CM, Shaw GS, Campbell AP, Sykes BD. A 1H NMR study of a ternary peptide complex that mimics the interaction between troponin C and troponin I. Protein Sci. 1992 Dec;1(12):1595-603. PubMed PMID: 1304891; PubMed Central PMCID: PMC2142143. 13: Campbell AP, Van Eyk JE, Hodges RS, Sykes BD. Interaction of troponin I and troponin C: use of the two-dimensional transferred nuclear Overhauser effect to determine the structure of a Gly-110 inhibitory troponin I peptide analog when bound to cardiac troponin C. Biochim Biophys Acta. 1992 Nov 10;1160(1):35-54. PubMed PMID: 1420332. 14: Ngai SM, Hodges RS. Biologically important interactions between synthetic peptides of the N-terminal region of troponin I and troponin C. J Biol Chem. 1992 Aug 5;267(22):15715-20. PubMed PMID: 1639807. 15: Campbell AP, Sykes BD. Interaction of troponin I and troponin C. Use of the two-dimensional nuclear magnetic resonance transferred nuclear Overhauser effect to determine the structure of the inhibitory troponin I peptide when bound to skeletal troponin C. J Mol Biol. 1991 Nov 20;222(2):405-21. PubMed PMID: 1960733. 16: Van Eyk JE, Kay CM, Hodges RS. A comparative study of the interactions of synthetic peptides of the skeletal and cardiac troponin I inhibitory region with skeletal and cardiac troponin C. Biochemistry. 1991 Oct 15;30(41):9974-81. PubMed PMID: 1911788. 17: Campbell AP, Cachia PJ, Sykes BD. Interaction of troponin I and troponin C: 19F NMR studies of the binding of the inhibitory troponin I peptide to turkey skeletal troponin C. Biochem Cell Biol. 1991 Sep;69(9):674-81. PubMed PMID: 1793571. 18: Lan J, Albaugh S, Steiner RF. Interactions of troponin I and its inhibitory fragment (residues 104-115) with troponin C and calmodulin. Biochemistry. 1989 Sep 5;28(18):7380-5. PubMed PMID: 2819077. 19: Rüegg JC, Zeugner C, Van Eyk J, Kay CM, Hodges RS. Inhibition of TnI-TnC interaction and contraction of skinned muscle fibres by the synthetic peptide TnI [104-115]. Pflugers Arch. 1989 Aug;414(4):430-6. PubMed PMID: 2798040. 20: Van Eyk JE, Hodges RS. The biological importance of each amino acid residue of the troponin I inhibitory sequence 104-115 in the interaction with troponin C and tropomyosin-actin. J Biol Chem. 1988 Feb 5;263(4):1726-32. PubMed PMID: 3338991.

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PCI-45752

PCI-45752