IUPAC/Chemical Name
(S)-N-((S)-1-(((S)-1-amino-5-guanidino-1-oxopentan-2-yl)(4-nitrophenyl)amino)-1-oxo-3-phenylpropan-2-yl)-1-benzoylpyrrolidine-2-carboxamide
InChi Key
GAMRKVOQYKERQN-KCHLEUMXSA-N
InChi Code
InChI=1S/C33H38N8O6/c34-29(42)27(13-7-19-37-33(35)36)40(24-15-17-25(18-16-24)41(46)47)32(45)26(21-22-9-3-1-4-10-22)38-30(43)28-14-8-20-39(28)31(44)23-11-5-2-6-12-23/h1-6,9-12,15-18,26-28H,7-8,13-14,19-21H2,(H2,34,42)(H,38,43)(H4,35,36,37)/t26-,27-,28-/m0/s1
SMILES Code
N=C(N)NCCC[C@@H](C(N)=O)N(C([C@H](CC1=CC=CC=C1)NC([C@H]2N(C(C3=CC=CC=C3)=O)CCC2)=O)=O)C4=CC=C([N+]([O-])=O)C=C4
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
Preparing Stock Solutions
The following data is based on the
product
molecular weight
642.71
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
| 1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
| 5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
| 10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
| 50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
1: Shibayama Y, Brunnee T, Kaplan AP, Reddigari S. Activation of human Hageman factor (factor XII) in the presence of zinc and phosphate ions. Braz J Med Biol Res. 1994 Aug;27(8):1817-28. PubMed PMID: 7749371.
2: Nishikawa K, Reddigari SR, Silverberg M, Kuna PB, Yago H, Nagaki Y, Toyomaki Y, Suehiro S, Kaplan AP. Effect of Neurotropin on the activation of the plasma kallikrein-kinin system. Biochem Pharmacol. 1992 Mar 17;43(6):1361-9. PubMed PMID: 1562287.
3: Tans G, Janssen-Claessen T, Rosing J, Griffin JH. Studies on the effect of serine protease inhibitors on activated contact factors. Application in amidolytic assays for factor XIIa, plasma kallikrein and factor XIa. Eur J Biochem. 1987 May 4;164(3):637-42. PubMed PMID: 3552674.
4: Nieuw Amerongen AV, Oderkerk CH, Roukema PA. AM1, a glycoprotein from the submandibular glands of the mouse, has esterolytic and amidolytic activities. Biochim Biophys Acta. 1984 May 17;786(3):245-51. PubMed PMID: 6722171.
5: Kyrle PA, Niessner H, Deutsch E, Lechner K, Korninger C, Mannhalter C. CRM+ severe Fletcher factor deficiency associated with Graves' disease. Haemostasis. 1984;14(4):302-6. PubMed PMID: 6568197.
6: Stürzebecher J, Klessen C, Markwardt F. [Determination of factor XII and plasma prekallikrein with chromozym PK]. Z Med Lab Diagn. 1982 Oct;23(5):306-11. German. PubMed PMID: 6924817.
7: Klessen C, Stürzebecher J, Markwardt F. Determination of factor XII in plasma using the kallikrein substrate chromozym PK. Thromb Res. 1982 Mar 15;25(6):501-5. PubMed PMID: 6921902.
8: Vogel G, Stürzebecher J, Klessen C, Lauten G. [Use of chromogenic substrates in the clarification of disorders in the early stages of blood coagulation]. Folia Haematol Int Mag Klin Morphol Blutforsch. 1982;109(1):115-20. German. PubMed PMID: 6177592.
9: Dick W, Cullmann W, Müller N, Adler K. Factor XII assay with the chromogenic substrate chromozym PK. J Clin Chem Clin Biochem. 1981 Jun;19(6):357-61. PubMed PMID: 6168728.
10: Ito R, Statland BE. Centrifugal analysis for plasma kallikrein activity, with use of the chromogenic substrate S-2302. Clin Chem. 1981 Apr;27(4):586-93. PubMed PMID: 6907061.
11: Aasen AO, Frolich W, Saugstad OD, Amundsen E. Plasma kallikrein activity and prekallikrein levels during endotoxin shock in dogs. Eur Surg Res. 1978;10(1):50-62. PubMed PMID: 344043.
12: Bang NU, Mattler LE. Sensitivity and specificity of plasma serine protease chromogenic substrates. Haemostasis. 1978;7(2-3):98-104. PubMed PMID: 149051.
13: Stormorken H, Baklund A, Gallimore M, Ritland S. Chromogenic substrate assay of plasma prekallikrein. With a note on its site of biosynthesis. Haemostasis. 1978;7(2-3):69-75. PubMed PMID: 26628.
14: Mattler LE, Bang NU. Serine protease specificity for peptide chromogenic substrates. Thromb Haemost. 1977 Dec 15;38(4):776-92. PubMed PMID: 146272.
