RO-3 | 1026582-88-6 | P2X3 and P2X2/3 antagonist

MedKoo Cat#: 555252 | Name: RO-3

Description:

WARNING: This product is for research use only, not for human or veterinary use.

RO-3 is a selective P2X3 and P2X2/3 antagonist.

Chemical Structure

RO-3

CAS#1026582-88-6

Theoretical Analysis

MedKoo Cat#: 555252

Name: RO-3

CAS#: 1026582-88-6

Chemical Formula: C16H22N4O2

Exact Mass: 302.1743

Molecular Weight: 302.38

Elemental Analysis: C, 63.55; H, 7.33; N, 18.53; O, 10.58

Price and Availability

Size	Price	Availability
100mg	USD 1,150.00	2 Weeks
200mg	USD 1,850.00	2 Weeks
500mg	USD 2,650.00	2 Weeks
1g	USD 3,850.00	2 Weeks
2g	USD 5,450.00	2 Weeks
5g	USD 9,450.00	2 Weeks

Bulk Inquiry

Buy Now

Add to Cart

Related CAS #

No Data

Synonym

RO-3; RO 3; RO3.

IUPAC/Chemical Name

5-(2-isopropyl-4,5-dimethoxybenzyl)pyrimidine-2,4-diamine

InChi Key

PYNPWUIBJMVRIG-UHFFFAOYSA-N

InChi Code

InChI=1S/C16H22N4O2/c1-9(2)12-7-14(22-4)13(21-3)6-10(12)5-11-8-19-16(18)20-15(11)17/h6-9H,5H2,1-4H3,(H4,17,18,19,20)

SMILES Code

NC1=NC=C(CC2=CC(OC)=C(OC)C=C2C(C)C)C(N)=N1

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

RO-3 is a dual antagonist of the purinergic receptor subtypes P2X3 and P2X2/3 (IC50s = 0.1 and 1.26 μM, respectively). It is selective for P2X3 and P2X2/3 over P2X1, P2X2, P2X4, P2X5, and P2X7 receptors (IC50s = >10 μM for all). RO-3 is an antagonist of ML1-type melatonin receptors (IC50 = 0.398 μM).

In vitro activity:

To be determined

In vivo activity:

This study found that in decerebrate cats, A-317491 and RO-3 reduced the pressor response to α,β-MeATP injection (before Δ 20 ± 3 mmHg; drug Δ 11 ± 3 mmHg; P < 0.05), suggesting the antagonists were effective in blocking P2X2/3 and P2X3 receptors. Reference: J Appl Physiol (1985). 2010 Nov;109(5):1416-23. https://pubmed.ncbi.nlm.nih.gov/20798273/

	Solvent	mg/mL	mM
Solubility
	DMF	20.0	66.14
	DMSO	12.0	39.69
	Ethanol	1.0	3.31

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 302.38 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. McCord JL, Tsuchimochi H, Kaufman MP. P2X2/3 and P2X3 receptors contribute to the metaboreceptor component of the exercise pressor reflex. J Appl Physiol (1985). 2010 Nov;109(5):1416-23. doi: 10.1152/japplphysiol.00774.2010. Epub 2010 Aug 26. PMID: 20798273; PMCID: PMC2980379.

In vitro protocol:

To be determined

In vivo protocol:

1: McCord JL, Tsuchimochi H, Kaufman MP. P2X2/3 and P2X3 receptors contribute to the metaboreceptor component of the exercise pressor reflex. J Appl Physiol (1985). 2010 Nov;109(5):1416-23. doi: 10.1152/japplphysiol.00774.2010. Epub 2010 Aug 26. PubMed PMID: 20798273; PubMed Central PMCID: PMC2980379.