MedKoo Biosciences, Inc.
Tel:   +1-919-636-5577    Fax:   +1-919-980-4831    Email:   sales@medkoo.com
Search
Login Register
Search
MedKoo Cat#: 571565 | Name: Rhodamine B
Featured

Description:

WARNING: This product is for research use only, not for human or veterinary use.

Rhodamine is from a family of 3,6-di(substituted-amino)-9-benzoate derivatives of xanthene that are used as dyes and as indicators for various metals; also used as fluorescent tracers in histochemistry.

Chemical Structure

Rhodamine B
Rhodamine B
CAS#81-88-9

Theoretical Analysis

MedKoo Cat#: 571565

Name: Rhodamine B

CAS#: 81-88-9

Chemical Formula: C28H31ClN2O3

Exact Mass: 0.0000

Molecular Weight: 479.02

Elemental Analysis: C, 70.21; H, 6.52; Cl, 7.40; N, 5.85; O, 10.02

Price and Availability

Size Price Availability Quantity
25g USD 205.00
100g USD 350.00
500g USD 640.00
Bulk Inquiry
Buy Now
Add to Cart
Related CAS #
No Data
Synonym
NSC 10475; NSC-10475; NSC10475; Symulex Magenta F; Basic Violet 10; Basonyl Red 545.
IUPAC/Chemical Name
9-(2-Carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride
InChi Key
PYWVYCXTNDRMGF-UHFFFAOYSA-N
InChi Code
InChI=1S/C28H30N2O3.ClH/c1-5-29(6-2)19-13-15-23-25(17-19)33-26-18-20(30(7-3)8-4)14-16-24(26)27(23)21-11-9-10-12-22(21)28(31)32;/h9-18H,5-8H2,1-4H3;1H
SMILES Code
CCN(C1=CC2=[O+]C3=C(C=CC(N(CC)CC)=C3)C(C4=CC=CC=C4C(O)=O)=C2C=C1)CC.[Cl-]
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
Rhodamine B is a dye.
In vitro activity:
Rhodamine B induced cytogenetic effects in A. cepa root tip cells, suggesting that the A. cepa root is an ideal model system for detecting cellular interactions. Reference: Environ Sci Pollut Res Int. 2014 Mar;21(5):3363-70. https://pubmed.ncbi.nlm.nih.gov/24234815/
In vivo activity:
In rats, rhodamine B was highly accumulated in the liver and, to a lesser extent, the kidney, but was undetectable in the brain. These results provide useful information for improving the pharmacokinetics and biodistribution of rhodamine B. Reference: J Agric Food Chem. 2017 Feb 8;65(5):1078-1085. https://pubmed.ncbi.nlm.nih.gov/28097866/
Solvent mg/mL mM
Solubility
DMSO 125.0 260.95
Water 100.0 208.76
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 479.02 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Tan D, Bai B, Jiang D, Shi L, Cheng S, Tao D, Ji S. Rhodamine B induces long nucleoplasmic bridges and other nuclear anomalies in Allium cepa root tip cells. Environ Sci Pollut Res Int. 2014 Mar;21(5):3363-70. doi: 10.1007/s11356-013-2282-9. PMID: 24234815. 2. Tong H, Jiang Q, Zhong X, Hu X. Rhodamine B dye staining for visualizing microplastics in laboratory-based studies. Environ Sci Pollut Res Int. 2021 Jan;28(4):4209-4215. doi: 10.1007/s11356-020-10801-4. Epub 2020 Sep 15. PMID: 32935213. 3. Cheng YY, Tsai TH. Pharmacokinetics and Biodistribution of the Illegal Food Colorant Rhodamine B in Rats. J Agric Food Chem. 2017 Feb 8;65(5):1078-1085. doi: 10.1021/acs.jafc.6b04975. Epub 2017 Jan 30. PMID: 28097866. 4. Roberts AL, Thomas BJ, Wilkinson AS, Fletcher JM, Byers S. Inhibition of glycosaminoglycan synthesis using rhodamine B in a mouse model of mucopolysaccharidosis type IIIA. Pediatr Res. 2006 Sep;60(3):309-14. doi: 10.1203/01.pdr.0000233037.00707.da. Epub 2006 Jul 20. PMID: 16857766.
In vitro protocol:
1. Tan D, Bai B, Jiang D, Shi L, Cheng S, Tao D, Ji S. Rhodamine B induces long nucleoplasmic bridges and other nuclear anomalies in Allium cepa root tip cells. Environ Sci Pollut Res Int. 2014 Mar;21(5):3363-70. doi: 10.1007/s11356-013-2282-9. PMID: 24234815. 2. Tong H, Jiang Q, Zhong X, Hu X. Rhodamine B dye staining for visualizing microplastics in laboratory-based studies. Environ Sci Pollut Res Int. 2021 Jan;28(4):4209-4215. doi: 10.1007/s11356-020-10801-4. Epub 2020 Sep 15. PMID: 32935213.
In vivo protocol:
1. Cheng YY, Tsai TH. Pharmacokinetics and Biodistribution of the Illegal Food Colorant Rhodamine B in Rats. J Agric Food Chem. 2017 Feb 8;65(5):1078-1085. doi: 10.1021/acs.jafc.6b04975. Epub 2017 Jan 30. PMID: 28097866. 2. Roberts AL, Thomas BJ, Wilkinson AS, Fletcher JM, Byers S. Inhibition of glycosaminoglycan synthesis using rhodamine B in a mouse model of mucopolysaccharidosis type IIIA. Pediatr Res. 2006 Sep;60(3):309-14. doi: 10.1203/01.pdr.0000233037.00707.da. Epub 2006 Jul 20. PMID: 16857766.
1: Oblinger JL, Wang J, Wetherell GD, Agarwal G, Wilson TA, Benson NR, Fenger JM, Fuchs JR, Kinghorn AD, Chang LS. Anti-tumor effects of the eIF4A inhibitor didesmethylrocaglamide and its derivatives in human and canine osteosarcomas. Sci Rep. 2024 Aug 20;14(1):19349. doi: 10.1038/s41598-024-69171-3. PMID: 39164287; PMCID: PMC11335891. 2: Oblinger J, Wang J, Wetherell G, Agarwal G, Wilson T, Benson N, Fenger J, Fuchs J, Kinghorn AD, Chang L. Anti-tumor Effects of the eIF4A Inhibitor Didesmethylrocaglamide and Its Derivatives in Human and Canine Osteosarcomas. Res Sq [Preprint]. 2024 Jun 14:rs.3.rs-4494024. doi: 10.21203/rs.3.rs-4494024/v1. Update in: Sci Rep. 2024 Aug 20;14(1):19349. doi: 10.1038/s41598-024-69171-3. PMID: 38947012; PMCID: PMC11213195. 3: Medica S, Crawford LB, Denton M, Min CK, Jones TA, Alexander T, Parkins CJ, Diggins NL, Streblow GJ, Mayo AT, Kreklywich CN, Smith P, Jeng S, McWeeney S, Hancock MH, Yurochko A, Cohen MS, Caposio P, Streblow DN. Proximity-dependent mapping of the HCMV US28 interactome identifies RhoGEF signaling as a requirement for efficient viral reactivation. PLoS Pathog. 2023 Oct 2;19(10):e1011682. doi: 10.1371/journal.ppat.1011682. PMID: 37782657; PMCID: PMC10569644. 4: Yamada S, Yassin MA, Torelli F, Hansmann J, Green JBA, Schwarz T, Mustafa K. Unique osteogenic profile of bone marrow stem cells stimulated in perfusion bioreactor is Rho-ROCK-mediated contractility dependent. Bioeng Transl Med. 2023 Mar 17;8(3):e10509. doi: 10.1002/btm2.10509. PMID: 37206242; PMCID: PMC10189446. 5: Li L, Xin J, Wang H, Wang Y, Peng W, Sun N, Huang H, Zhou Y, Liu X, Lin Y, Fang J, Jing B, Pan K, Zeng Y, Zeng D, Qin X, Bai Y, Ni X. Fluoride disrupts intestinal epithelial tight junction integrity through intracellular calcium- mediated RhoA/ROCK signaling and myosin light chain kinase. Ecotoxicol Environ Saf. 2023 Jun 1;257:114940. doi: 10.1016/j.ecoenv.2023.114940. Epub 2023 Apr 24. PMID: 37099960. 6: Dandamudi A, Seibel W, Tourdot B, Cancelas JA, Akbar H, Zheng Y. Structure- Activity Relationship Analysis of Rhosin, a RhoA GTPase Inhibitor, Reveals a New Class of Antiplatelet Agents. Int J Mol Sci. 2023 Feb 19;24(4):4167. doi: 10.3390/ijms24044167. PMID: 36835579; PMCID: PMC9961652. 7: Beak M, Park S, Kim JH, Eom HJ, Lee HY, Kim YH, Lee J, Nam S. Second- Generation JK-206 Targets the Oncogenic Signal Mediator RHOA in Gastric Cancer. Cancers (Basel). 2022 Mar 22;14(7):1604. doi: 10.3390/cancers14071604. PMID: 35406376; PMCID: PMC8997135. 8: Liu H, Zeng X, Ma Y, Chen X, Losiewicz MD, Du X, Tian Z, Zhang S, Shi L, Zhang H, Yang F. Long-term exposure to low concentrations of MC-LR induces blood-testis barrier damage through the RhoA/ROCK pathway. Ecotoxicol Environ Saf. 2022 May 1;236:113454. doi: 10.1016/j.ecoenv.2022.113454. Epub 2022 Mar 31. PMID: 35367887. 9: Zhang D, Zhu Y, Li Z, Luo M, Liang X, Wang A, Zhu H, Hu L, Li R. The role of Astragalus polysaccharides in promoting IEC-6 cell migration from polyamine- mediated Ca2+ regulation. Int J Biol Macromol. 2022 May 15;207:179-192. doi: 10.1016/j.ijbiomac.2022.02.109. Epub 2022 Feb 23. PMID: 35217086. 10: Somodi L, Beke Debreceni I, Kis G, Cozzolino M, Kappelmayer J, Antal M, Panyi G, Bárdos H, Mutch NJ, Muszbek L. Activation mechanism dependent surface exposure of cellular factor XIII on activated platelets and platelet microparticles. J Thromb Haemost. 2022 May;20(5):1223-1235. doi: 10.1111/jth.15668. Epub 2022 Feb 21. PMID: 35146910; PMCID: PMC9303193. 11: Shivangi, Meena LS. A multifactorial assessment of the SRP pathway constituent FtsY as a vital mycobacterial constituent. Biotechnol Appl Biochem. 2022 Dec;69(6):2445-2453. doi: 10.1002/bab.2294. Epub 2022 Jan 8. PMID: 34837716. 12: Okamoto Y, Kitakaze K, Takenouchi Y, Yamamoto S, Ishimaru H, Tsuboi K. Sphingosine 1-phosphate receptor type 2 positively regulates interleukin (IL)-4/IL-13-induced STAT6 phosphorylation. Cell Signal. 2021 Dec;88:110156. doi: 10.1016/j.cellsig.2021.110156. Epub 2021 Sep 28. PMID: 34592416. 13: Sundberg M, Pinson H, Smith RS, Winden KD, Venugopal P, Tai DJC, Gusella JF, Talkowski ME, Walsh CA, Tegmark M, Sahin M. 16p11.2 deletion is associated with hyperactivation of human iPSC-derived dopaminergic neuron networks and is rescued by RHOA inhibition in vitro. Nat Commun. 2021 May 18;12(1):2897. doi: 10.1038/s41467-021-23113-z. PMID: 34006844; PMCID: PMC8131375. 14: Chiu WC, Chiang JY, Chiang FT. Small chemical compounds Y16 and Rhosin can inhibit calcium sensitization pathway in vascular smooth muscle cells of spontaneously hypertensive rats. J Formos Med Assoc. 2021 Oct;120(10):1863-1868. doi: 10.1016/j.jfma.2021.03.031. Epub 2021 Apr 21. PMID: 33893012. 15: Devi SS, Yadav R, Arya R. Altered Actin Dynamics in Cell Migration of GNE Mutant Cells. Front Cell Dev Biol. 2021 Mar 18;9:603742. doi: 10.3389/fcell.2021.603742. PMID: 33816461; PMCID: PMC8012676. 16: Tsubaki M, Genno S, Takeda T, Matsuda T, Kimura N, Yamashita Y, Morii Y, Shimomura K, Nishida S. Rhosin Suppressed Tumor Cell Metastasis through Inhibition of Rho/YAP Pathway and Expression of RHAMM and CXCR4 in Melanoma and Breast Cancer Cells. Biomedicines. 2021 Jan 4;9(1):35. doi: 10.3390/biomedicines9010035. PMID: 33406809; PMCID: PMC7824767. 17: Ariyani W, Miyazaki W, Amano I, Hanamura K, Shirao T, Koibuchi N. Soy Isoflavones Accelerate Glial Cell Migration via GPER-Mediated Signal Transduction Pathway. Front Endocrinol (Lausanne). 2020 Nov 4;11:554941. doi: 10.3389/fendo.2020.554941. PMID: 33250856; PMCID: PMC7672195. 18: Privat M, Cavard A, Zekri Y, Ponelle-Chachuat F, Molnar I, Sonnier N, Bignon YJ. A high expression ratio of RhoA/RhoB is associated with the migratory and invasive properties of basal-like Breast Tumors. Int J Med Sci. 2020 Oct 1;17(17):2799-2808. doi: 10.7150/ijms.43101. PMID: 33162807; PMCID: PMC7645338. 19: Abramochkin DV, Filatova TS, Pustovit KB, Dzhumaniiazova I, Karpushev AV. Small G-protein RhoA is a potential inhibitor of cardiac fast sodium current. J Physiol Biochem. 2021 Feb;77(1):13-23. doi: 10.1007/s13105-020-00774-w. Epub 2020 Nov 4. PMID: 33145656. 20: Kim JH, Park S, Lim SM, Eom HJ, Balch C, Lee J, Kim GJ, Jeong JH, Nam S, Kim YH. Rational design of small molecule RHOA inhibitors for gastric cancer. Pharmacogenomics J. 2020 Aug;20(4):601-612. doi: 10.1038/s41397-020-0153-6. Epub 2020 Feb 4. PMID: 32015453.

View History

Dipfluzine

Dipfluzine