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MedKoo Cat#: 571542 | Name: Ponceau MX
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Ponceau MX is a dye, which may be used in biochemistry study.

Chemical Structure

Ponceau MX
Ponceau MX
CAS#3761-53-3

Theoretical Analysis

MedKoo Cat#: 571542

Name: Ponceau MX

CAS#: 3761-53-3

Chemical Formula: C18H14N2Na2O7S2

Exact Mass:

Molecular Weight: 480.42

Elemental Analysis: C, 45.00; H, 2.94; N, 5.83; Na, 9.57; O, 23.31; S, 13.35

Price and Availability

Size Price Availability Quantity
25g USD 230.00
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Synonym
Ponceau MX; Xylidine Red; Acid Red 26; Ponceau xylidine; Acid Red 26, Ponceau 2 R; Xylidine ponceau 2R, Xylidine ponceau;
IUPAC/Chemical Name
2,7-Naphthalenedisulfonic acid, 3-hydroxy-4-(2,4-xylylazo)-, disodium salt
InChi Key
YJVBLROMQZEFPA-LLIZZRELSA-L
InChi Code
InChI=1S/C18H16N2O7S2.2Na/c1-10-3-6-15(11(2)7-10)19-20-17-14-5-4-13(28(22,23)24)8-12(14)9-16(18(17)21)29(25,26)27;;/h3-9,21H,1-2H3,(H,22,23,24)(H,25,26,27);;/q;2*+1/p-2/b20-19+;;
SMILES Code
O=S(C1=C(O)C(/N=N/C2=CC=C(C)C=C2C)=C3C=CC(S(=O)([O-])=O)=CC3=C1)([O-])=O.[Na+].[Na+]
Appearance
Solid powder
Purity
>60% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
Ponceau MX is a dye.
In vitro activity:
In this study, this study proposed a two-dimensional (2D) Karnovsky-Roots toluidine blue ponceau 2R (K-B-2R) staining method based upon conventional Karnovsky-Roots staining. It significantly improved the ability to display nerve fascicles, motor and sensory nerve fiber textures. In this method, Karnovsky-Roots staining was carried out, followed by toluidine blue counterstain and ponceau 2R counterstain. Reference: BMC Neurosci. 2019 Jul 8;20(1):32. https://pubmed.ncbi.nlm.nih.gov/31286881/
In vivo activity:
In this study, the toxicity of these dyes was assessed in a zebrafish model, and the underlying toxic mechanisms were investigated. Treatment with Basic Violet 14, Direct Red 28 and Acid Red 26 resulted in common developmental abnormalities including delayed yolk sac absorption and swimming bladder deflation. Hepatotoxicity was observed in zebrafish treated with Basic Violet 14, and cardiovascular toxicity was found in zebrafish treated with Acid Red 26 at concentrations higher than 2500 µg ml(-1) . Basic Violet 14 also caused significant up-regulation of GCLC gene expression in a dose-dependent manner whereas Acid Red 26 induced significant up-regulation of NKX2.5 and down-regulation of GATA4 at a high concentration in a dose-dependent manner. Reference: J Appl Toxicol. 2015 Dec;35(12):1473-80. https://pubmed.ncbi.nlm.nih.gov/25727789/

Preparing Stock Solutions

The following data is based on the product molecular weight 480.42 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Luo P, Dong J, Qi J, Zhang Y, Liu X, Zhong Y, Xian CJ, Wang L. An enhanced staining method K-B-2R staining for three-dimensional nerve reconstruction. BMC Neurosci. 2019 Jul 8;20(1):32. doi: 10.1186/s12868-019-0515-7. PMID: 31286881; PMCID: PMC6615204. 2. Shen B, Liu HC, Ou WB, Eilers G, Zhou SM, Meng FG, Li CQ, Li YQ. Toxicity induced by Basic Violet 14, Direct Red 28 and Acid Red 26 in zebrafish larvae. J Appl Toxicol. 2015 Dec;35(12):1473-80. doi: 10.1002/jat.3134. Epub 2015 Feb 27. PMID: 25727789.
In vitro protocol:
Luo P, Dong J, Qi J, Zhang Y, Liu X, Zhong Y, Xian CJ, Wang L. An enhanced staining method K-B-2R staining for three-dimensional nerve reconstruction. BMC Neurosci. 2019 Jul 8;20(1):32. doi: 10.1186/s12868-019-0515-7. PMID: 31286881; PMCID: PMC6615204.
In vivo protocol:
Shen B, Liu HC, Ou WB, Eilers G, Zhou SM, Meng FG, Li CQ, Li YQ. Toxicity induced by Basic Violet 14, Direct Red 28 and Acid Red 26 in zebrafish larvae. J Appl Toxicol. 2015 Dec;35(12):1473-80. doi: 10.1002/jat.3134. Epub 2015 Feb 27. PMID: 25727789.
1: Goel A, Lasyal R. Iridium nanoparticles with high catalytic activity in degradation of acid red-26: an oxidative approach. Water Sci Technol. 2016 Dec;74(11):2551-2559. PubMed PMID: 27973360. 2: Xing X, Qu H, Chen P, Chi B, Xie H. Studies on competitive adsorption of dyes onto carbon (XC-72) and regeneration of adsorbent. Water Sci Technol. 2016 Nov;74(10):2505-2514. PubMed PMID: 27858807. 3: Xu F, Mou Z, Geng J, Zhang X, Li CZ. Azo dye decolorization by a halotolerant exoelectrogenic decolorizer isolated from marine sediment. Chemosphere. 2016 Sep;158:30-6. doi: 10.1016/j.chemosphere.2016.05.033. Epub 2016 May 27. PubMed PMID: 27239968. 4: Shen B, Liu HC, Ou WB, Eilers G, Zhou SM, Meng FG, Li CQ, Li YQ. Toxicity induced by Basic Violet 14, Direct Red 28 and Acid Red 26 in zebrafish larvae. J Appl Toxicol. 2015 Dec;35(12):1473-80. doi: 10.1002/jat.3134. Epub 2015 Feb 27. PubMed PMID: 25727789. 5: Mouso N, Diorio L, Forchiassin F. [Stereum hirsutum (Wild) Pers. action in dye degradation]. Rev Iberoam Micol. 2007 Dec 31;24(4):294-8. Spanish. PubMed PMID: 18095763. 6: Parodi S, Zunino A, Ottaggio L, De Ferrari M, Santi L. Lack of correlation between the capability of inducing sister-chromatid exchanges in vivo and carcinogenic potency, for 16 aromatic amines and azo derivatives. Mutat Res. 1983 Mar;108(1-3):225-38. PubMed PMID: 6835220. 7: Pimentel ER, Recco SM, Graccho M Jr. Photometric studies on Xylidine Ponceau-collagen interaction. Cell Mol Biol Incl Cyto Enzymol. 1981;27(4):347-52. PubMed PMID: 7317916. 8: Parodi S, Taningher M, Russo P, Pala M, Tamaro M, Monti-Bragadin C. DNA-damaging activity in vivo and bacterial mutagenicity of sixteen aromatic amines and azo-derivatives, as related quantitatively to their carcinogenicity. Carcinogenesis. 1981;2(12):1317-26. PubMed PMID: 7034987. 9: Messelt EB. A new differential staining method of semithin sections of polyester-embedded salivary glands. Acta Odontol Scand. 1981;39(2):67-70. PubMed PMID: 6172953. 10: Grasso P. Liver growth and tumorigenesis in rats. Arch Toxicol Suppl. 1979;(2):171-80. PubMed PMID: 288328. 11: Grasso P, Gray TJ. Long-term studies on chemically induced liver enlargement in the rat. III. Structure and behaviour of the hepatic nodular lesions induced by Ponceau MX. Toxicology. 1977 Jun;7(3):327-47. PubMed PMID: 888149. 12: Crampton RF, Gray TJ, Grasso P, Parke DV. Long-term studies on chemically induced liver enlargement in the rat. II. Transient induction of microsomal enzymes leading to liver damage and nodular hyperplasia produced by safrole and Ponceau MX. Toxicology. 1977 Jun;7(3):307-26. PubMed PMID: 888148. 13: Grasso P, Lansdown AB, Kiss IS, Gaunt IF, Gangolli SD. Nodular hyperplasia in the rat liver following prolonged feeding of Ponceau MX. Food Cosmet Toxicol. 1969 Sep;7(5):425-42. PubMed PMID: 5351707. 14: Ikeda Y, Horiuchi S, Kobayashi K, Furuja T, Kohgo K. Carcinogenicity of Ponceau MX in the mouse. Food Cosmet Toxicol. 1968 Dec;6(5):591-8. PubMed PMID: 5732320. 15: Grasso P. Carcinogenicity of ponceau MX in the mouse. Food Cosmet Toxicol. 1968 Dec;6(6):821-2. PubMed PMID: 4308811. 16: Ikeda Y, Horiuchi S, Furuya T, Omori Y. Chronic toxicity of Ponceau MX in the rat. Food Cosmet Toxicol. 1966 Oct;4(5):485-92. PubMed PMID: 5971935. 17: Hall DE, Lee FS, Fairweather FA. Acute (mouse and rat) and short-term (rat) toxicity studies on Ponceau MX. Food Cosmet Toxicol. 1966 Aug;4(4):375-82. PubMed PMID: 5975243. 18: Ikeda Y. Long-term study of Ponceau MX in rats and mice. Food Cosmet Toxicol. 1966 Jun;4(3):361-2. PubMed PMID: 5962926.