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MedKoo Cat#: 571639 | Name: Biotin-HPDP
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Biotin-HPDP, also known as N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)-propionamide, is a sulfhydryl reactive biotinylation reagent with a long chain, cleavable spacer arm.

Chemical Structure

Biotin-HPDP
Biotin-HPDP
CAS#129179-83-5

Theoretical Analysis

MedKoo Cat#: 571639

Name: Biotin-HPDP

CAS#: 129179-83-5

Chemical Formula: C24H37N5O3S3

Exact Mass: 539.2059

Molecular Weight: 539.77

Elemental Analysis: C, 53.40; H, 6.91; N, 12.97; O, 8.89; S, 17.82

Price and Availability

Size Price Availability Quantity
50mg USD 450.00 2 Weeks
100mg USD 750.00 2 Weeks
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Related CAS #
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Synonym
Biotin-HPDP, Biotin HPDP, Pyridyldithiol-biotin, Pyridyldisulfide-biotin
IUPAC/Chemical Name
N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)-propionamide
InChi Key
QLPHBNRMJLFRGO-YDHSSHFGSA-N
InChi Code
InChI=1S/C24H37N5O3S3/c30-20(10-4-3-9-19-23-18(17-33-19)28-24(32)29-23)25-13-6-1-2-7-14-26-21(31)12-16-34-35-22-11-5-8-15-27-22/h5,8,11,15,18-19,23H,1-4,6-7,9-10,12-14,16-17H2,(H,25,30)(H,26,31)(H2,28,29,32)/t18-,19-,23-/m0/s1
SMILES Code
O=C(NCCCCCCNC(CCCC[C@H]1[C@]([H])([C@]([H])(CS1)N2)NC2=O)=O)CCSSC3=NC=CC=C3
Appearance
Purity
>95% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Biotin-HPDP biotinylation occurs between pH 6-9 through its pyridyldithiol group reacting with sulfhydryls to form a stable disulfide bond. The 29.2 angstrom spacer arm prevents steric hindrance and may also be cleaved at the disulfide bond using TCEP-HCl, DTT or ß-Mercaptoethanol. Biotin-HPDP must be dissolved in an organic solvent, such as DMSO or DMF, then added to an aqueous biotinylation reaction. The resulting biotinylated protein can then be detected when used in conjunction with streptavidin or Immobilized Streptavidin.

Preparing Stock Solutions

The following data is based on the product molecular weight 539.77 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1: Kim NH, Baek TJ, Park HG, Seong GH. Highly sensitive biomolecule detection on a quartz crystal microbalance using gold nanoparticles as signal amplification probes. Anal Sci. 2007 Feb;23(2):177-81. PubMed PMID: 17297229. 2: Brown RJ, Brett DJ. Electrochemical study of biotin-modified self-assembled monolayers: recommendations for robust preparation. ScientificWorldJournal. 2006 Jan 17;6:20-9. PubMed PMID: 16432625. 3: Wan J, Roth AF, Bailey AO, Davis NG. Palmitoylated proteins: purification and identification. Nat Protoc. 2007;2(7):1573-84. PubMed PMID: 17585299. 4: Shu W, Laue ED, Seshia AA. Investigation of biotin-streptavidin binding interactions using microcantilever sensors. Biosens Bioelectron. 2007 Apr 15;22(9-10):2003-9. Epub 2006 Oct 12. PubMed PMID: 17045792.