Sulfo-NHS-LC-Biotin | CAS# 127062-22-0 | Biotinylating Reagent

MedKoo Cat#: 571638 | Name: Sulfo-NHS-LC-Biotin

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Sulfo-NHS-LC-Biotin, or Sulfosuccinimidyl 6-(biotinamido) Hexanoate, is a long chain, water soluble biotinylation reagent that is reactive toward primary amine groups. Similar to Sulfo-NHS-Biotin, Sulfo-NHS-LC-Biotin is designed to target cell surface proteins.

Chemical Structure

Sulfo-NHS-LC-Biotin

CAS#127062-22-0

Theoretical Analysis

MedKoo Cat#: 571638

Name: Sulfo-NHS-LC-Biotin

CAS#: 127062-22-0

Chemical Formula: C20H30N4O9S2

Exact Mass: 534.1454

Molecular Weight: 534.60

Elemental Analysis: C, 44.93; H, 5.66; N, 10.48; O, 26.93; S, 11.99

Price and Availability

Size	Price	Availability	Quantity
100mg	USD 340.00
1g	USD 1,200.00

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Synonym

Sulfo-NHS-LC-Biotin, Biotin-X-NHS (Water Soluble), Sulfo NHS LC Biotin, long chain Sulfo NHS Biotin

IUPAC/Chemical Name

Sulfosuccinimidyl 6-(biotinamido) Hexanoate

InChi Key

UQZHJQWIISKTJN-YALINYFNSA-N

InChi Code

InChI=1S/C20H30N4O9S2/c25-15(7-4-3-6-13-18-12(11-34-13)22-20(29)23-18)21-9-5-1-2-8-17(27)33-24-16(26)10-14(19(24)28)35(30,31)32/h12-14,18H,1-11H2,(H,21,25)(H2,22,23,29)(H,30,31,32)/t12-,13-,14?,18-/m0/s1

SMILES Code

O=C(ON1C(C(S(=O)(O)=O)CC1=O)=O)CCCCCNC(CCCC[C@H]2[C@]([H])([C@]([H])(CS2)N3)NC3=O)=O

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Similar to Sulfo-NHS-Biotin, Sulfo-NHS-LC-Biotin is designed to target cell surface proteins. The sodium sulfonate groups lend water solubility to Sulfo-NHS-LC-Biotin and prevent it from crossing the lipid bi-layer of the cell membrane; whereas NHS-LC-Biotin can biotinylate intracellular proteins. The resulting biotinylated protein can then be detected when used in conjunction with streptavidin and Immobilized Streptavidin. The "long chain" Sulfo-NHS-LC-Biotin and Sulfo-NHS-Biotin both have the same chemical functionality. However, Sulfo-NHS-LC-Biotin will provide a longer spacer arm that is designed to overcome any steric hindrances that may be present between the labeled protein(s) and streptavidin

Instruction

View handling instruction

Preparing Stock Solutions

The following data is based on the product molecular weight 534.60 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

1: Gabant G, Augier J, Armengaud J. Assessment of solvent residues accessibility using three Sulfo-NHS-biotin reagents in parallel: application to footprint changes of a methyltransferase upon binding its substrate. J Mass Spectrom. 2008 Mar;43(3):360-70. PubMed PMID: 17968972.