NHS-SS-Biotin, or Succinimidyl-2-(biotinamido)-ethyl-1,3'-dithiopropionate, is an amine-reactive biotinylation reagent that is cleavable when the disulfide (-S-S-) bond is reduced. The resulting biotinylated protein can then be detected when used in conjunction with streptavidin. Therefore, NHS-SS-Biotin can be useful in antibody purification.
MedKoo Cat#: 571636
Name: NHS-SS-Biotin
CAS#: 142439-92-7
Chemical Formula: C19H28N4O6S3
Exact Mass: 504.1171
Molecular Weight: 504.64
Elemental Analysis: C, 45.22; H, 5.59; N, 11.10; O, 19.02; S, 19.06
The following data is based on the product molecular weight 504.64 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
