MedKoo Cat#: 571634 | Name: NHS-Biotin
Featured

Description:

WARNING: This product is for research use only, not for human or veterinary use.

NHS-Biotin, Succinimidobiotin, is a primary amine reactive biotinylation reagent used in Biotin-Streptavidin binding reactions. The NHS-Biotin molecule is cell membrane permeable and can be used to covalently label intracellular proteins.

Chemical Structure

NHS-Biotin
CAS#35013-72-0

Theoretical Analysis

MedKoo Cat#: 571634

Name: NHS-Biotin

CAS#: 35013-72-0

Chemical Formula: C14H19N3O5S

Exact Mass: 341.1045

Molecular Weight: 341.38

Elemental Analysis: C, 49.26; H, 5.61; N, 12.31; O, 23.43; S, 9.39

Price and Availability

Size Price Availability Quantity
100mg USD 255.00
1g USD 900.00
Bulk Inquiry
Buy Now
Add to Cart
Related CAS #
No Data
Synonym
NHS-Biotin, Succinimidobiotin, Biotin-OSU; Biotin-NHS ester
IUPAC/Chemical Name
Biotin N-hydroxysuccinimide ester
InChi Key
YMXHPSHLTSZXKH-RVBZMBCESA-N
InChi Code
InChI=1S/C14H19N3O5S/c18-10-5-6-11(19)17(10)22-12(20)4-2-1-3-9-13-8(7-23-9)15-14(21)16-13/h8-9,13H,1-7H2,(H2,15,16,21)/t8-,9-,13-/m0/s1
SMILES Code
O=C1N[C@@]([H])([C@]([H])(CS2)N1)[C@@H]2CCCCC(ON3C(CCC3=O)=O)=O
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
The water soluble version of NHS-Biotin is Sulfo-NHS-Biotin.

Preparing Stock Solutions

The following data is based on the product molecular weight 341.38 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1: Gabant G, Augier J, Armengaud J. Assessment of solvent residues accessibility using three Sulfo-NHS-biotin reagents in parallel: application to footprint changes of a methyltransferase upon binding its substrate. J Mass Spectrom. 2008 Mar;43(3):360-70. PubMed PMID: 17968972. 2: Pintado CO, Moreno A, Barbancho M, Pérez de la Lastra JM, Llanes D. The beta chain of the GPIIb molecule on ruminant leukocytes and platelets is not labelled by the sulfo-NHS-biotin method. Vox Sang. 1995;69(3):248-9. PubMed PMID: 8578739.