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MedKoo Cat#: 571600 | Name: ANB-NOS Crosslinker
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

ANB-NOS Crosslinker, also known as N-5-Azido-2-nitrobenzoyloxysuccinimide, is a heterobifunctional protein crosslinker with a spacer arm length of 7.7 angstroms. The ANB-NOS crosslinking reagent contains an NHS ester that reacts with primary amines and a nitrophenylazide group that is photoreactive at 320-350 nm toward amino groups. ANB-NOS Crosslinker is useful for making ADCs (antibody drug conjugates).

Chemical Structure

ANB-NOS Crosslinker
ANB-NOS Crosslinker
CAS#60117-35-3

Theoretical Analysis

MedKoo Cat#: 571600

Name: ANB-NOS Crosslinker

CAS#: 60117-35-3

Chemical Formula: C11H7N5O6

Exact Mass: 305.0396

Molecular Weight: 305.21

Elemental Analysis: C, 43.29; H, 2.31; N, 22.95; O, 31.45

Price and Availability

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100mg USD 350.00 2 Weeks
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Synonym
ANB-NOS Crosslinker
IUPAC/Chemical Name
5-Azido-2-nitrobenzoic acid N-hydroxysuccinimide ester
InChi Key
FUOJEDZPVVDXHI-UHFFFAOYSA-N
InChi Code
InChI=1S/C11H7N5O6/c12-14-13-6-1-2-8(16(20)21)7(5-6)11(19)22-15-9(17)3-4-10(15)18/h1-2,5H,3-4H2
SMILES Code
O=C(ON1C(CCC1=O)=O)C2=CC(N=[N+]=[N-])=CC=C2[N+]([O-])=O
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info

Preparing Stock Solutions

The following data is based on the product molecular weight 305.21 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1: Mehravi B, Ahmadi M, Amanlou M, Mostaar A, Ardestani MS, Ghalandarlaki N. Conjugation of glucosamine with Gd3+-based nanoporous silica using a heterobifunctional ANB-NOS crosslinker for imaging of cancer cells. Int J Nanomedicine. 2013;8:3383-94. doi: 10.2147/IJN.S44829. Epub 2013 Sep 24. PMID: 24101868; PMCID: PMC3790884. 2: Nadeau OW, Carlson GM. Protein Interactions Captured by Chemical Cross- linking: Two-Step Cross-linking with ANB*NOS. CSH Protoc. 2007 Apr 1;2007:pdb.prot4635. doi: 10.1101/pdb.prot4635. PMID: 21357054. 3: Hoi H, Rezaie SS, Gong L, Sen P, Zeng H, Montemagno C, Gupta M. Biofunctionalized silicon nitride platform for sensing applications. Biosens Bioelectron. 2018 Apr 15;102:497-503. doi: 10.1016/j.bios.2017.11.059. Epub 2017 Nov 23. PMID: 29197282. 4: Dusseault J, Leblond FA, Robitaille R, Jourdan G, Tessier J, Ménard M, Henley N, Hallé JP. Microencapsulation of living cells in semi-permeable membranes with covalently cross-linked layers. Biomaterials. 2005 May;26(13):1515-22. doi: 10.1016/j.biomaterials.2004.05.013. PMID: 15522753. 5: Ibupoto ZH, Ali SM, Khun K, Chey CO, Nur O, Willander M. ZnO Nanorods Based Enzymatic Biosensor for Selective Determination of Penicillin. Biosensors (Basel). 2011 Oct 27;1(4):153-63. doi: 10.3390/bios1040153. PMID: 25585565; PMCID: PMC4264348. 6: Jarvis LJ, Louis CF. Purification and oligomeric state of the major lens fiber cell membrane proteins. Curr Eye Res. 1995 Sep;14(9):799-808. doi: 10.3109/02713689508995802. PMID: 8529419. 7: Chen LL, Lobb RR, Cuervo JH, Lin Kc, Adams SP, Pepinsky RB. Identification of ligand binding sites on integrin alpha4beta1 through chemical cross-linking. Biochemistry. 1998 Jun 16;37(24):8743-53. doi: 10.1021/bi980311a. PMID: 9628736. 8: McMahan SA, Burgess RR. Use of aryl azide cross-linkers to investigate protein-protein interactions: an optimization of important conditions as applied to Escherichia coli RNA polymerase and localization of a sigma 70-alpha cross- link to the C-terminal region of alpha. Biochemistry. 1994 Oct 11;33(40):12092-9. doi: 10.1021/bi00206a012. PMID: 7918430. 9: Kwon H, Hardwicke PM, Collins JH, Zhao X, Szent-Györgyi AG. Myosin filament ATPase is enhanced by intramolecularly cross-linked actin. J Muscle Res Cell Motil. 1994 Oct;15(5):555-62. doi: 10.1007/BF00121161. PMID: 7860703. 10: Moghaddam MJ, Yang W, Bojarski B, Gengenbach TR, Gao M, Zareie H, McCall MJ. Azide photochemistry for facile modification of graphitic surfaces: preparation of DNA-coated carbon nanotubes for biosensing. Nanotechnology. 2012 Oct 26;23(42):425503. doi: 10.1088/0957-4484/23/42/425503. Epub 2012 Oct 4. Erratum in: Nanotechnology. 2013 Sep 20;24(37):379501. PMID: 23037575. 11: Karrasch S, Dolder M, Schabert F, Ramsden J, Engel A. Covalent binding of biological samples to solid supports for scanning probe microscopy in buffer solution. Biophys J. 1993 Dec;65(6):2437-46. doi: 10.1016/S0006-3495(93)81327-4. PMID: 8312482; PMCID: PMC1225984. 12: Green A, Stuart CA, Pietrzyk RA, Partin M. Photochemical cross-linking of 125I-hydroxyphenylisopropyl adenosine to the A1 adenosine receptor of rat adipocytes. FEBS Lett. 1986 Sep 29;206(1):130-4. doi: 10.1016/0014-5793(86)81354-0. PMID: 3758344. 13: Wang P, Gyllner G, Kvist S. Selection and binding of peptides to human transporters associated with antigen processing and rat cim-a and -b. J Immunol. 1996 Jul 1;157(1):213-20. PMID: 8683118. 14: Buckland PR, Rickards CR, Howells RD, Smith BR. Thyrotropin cross-links to the thyrotropin receptor through both the alpha and beta subunits. Biochem J. 1986 May 1;235(3):879-82. doi: 10.1042/bj2350879. PMID: 3019309; PMCID: PMC1146768. 15: Nadeau OW, Traxler KW, Fee LR, Baldwin BA, Carlson GM. Activators of phosphorylase kinase alter the cross-linking of its catalytic subunit to the C-terminal one-sixth of its regulatory alpha subunit. Biochemistry. 1999 Feb 23;38(8):2551-9. doi: 10.1021/bi982060b. PMID: 10029550. 16: Sheikh SP, Williams JA. Structural characterization of Y1 and Y2 receptors for neuropeptide Y and peptide YY by affinity cross-linking. J Biol Chem. 1990 May 15;265(14):8304-10. PMID: 2159475. 17: Kimura N. Developmental change and molecular properties of somatostatin receptors in the rat cerebral cortex. Biochem Biophys Res Commun. 1989 Apr 14;160(1):72-8. doi: 10.1016/0006-291x(89)91622-7. PMID: 2565720. 18: Lewis LD, Williams JA. Structural characterization of the somatostatin receptor in rat anterior pituitary membranes. Endocrinology. 1987 Aug;121(2):486-92. doi: 10.1210/endo-121-2-486. PMID: 2885181. 19: Nagao M, Sakamoto C, Matozaki T, Nishizaki H, Konda Y, Nakano O, Baba S. [Structural characterization of the somatostatin receptors on rat cerebrocortical membranes]. Nihon Naibunpi Gakkai Zasshi. 1990 Oct 20;66(10):1108-16. Japanese. doi: 10.1507/endocrine1927.66.10_1108. PMID: 1980894.