MedKoo Biosciences, Inc.
Tel:   +1-919-636-5577    Fax:   +1-919-980-4831    Email:   sales@medkoo.com
Search
Login Register
Search
MedKoo Cat#: 562562 | Name: PS-48
Featured

Description:

WARNING: This product is for research use only, not for human or veterinary use.

PS-48 is a phosphoinositide-dependent protein kinase-1 (PDK1) activator. Treatment of low cloning efficiency BFFs with PS48 resulted in an increase in the intracellular lactate production and H3K9 acetylation, decrease in histone deacetylase activity and HP1α expression, less condensed chromatin structure and more cloning embryos developing to blastocysts.

Chemical Structure

PS-48
PS-48
CAS#1180676-32-7

Theoretical Analysis

MedKoo Cat#: 562562

Name: PS-48

CAS#: 1180676-32-7

Chemical Formula: C17H15ClO2

Exact Mass: 286.0761

Molecular Weight: 286.75

Elemental Analysis: C, 71.21; H, 5.27; Cl, 12.36; O, 11.16

Price and Availability

Size Price Availability Quantity
50mg USD 550.00 2 Weeks
100mg USD 950.00 2 Weeks
200mg USD 1,650.00 2 Weeks
500mg USD 2,650.00 2 Weeks
1g USD 3,250.00 2 Weeks
2g USD 5,650.00 2 Weeks
Show More
Bulk Inquiry
Buy Now
Add to Cart
Related CAS #
No Data
Synonym
PS-48; PS 48; PS48;
IUPAC/Chemical Name
(2Z)-5-(4-Chlorophenyl)-3-phenyl-2-pentenoic acid
InChi Key
LLJYFDRQFPQGNY-QINSGFPZSA-N
InChi Code
InChI=1S/C17H15ClO2/c18-16-10-7-13(8-11-16)6-9-15(12-17(19)20)14-4-2-1-3-5-14/h1-5,7-8,10-12H,6,9H2,(H,19,20)/b15-12-
SMILES Code
O=C(O)/C=C(C1=CC=CC=C1)/CCC2=CC=C(Cl)C=C2
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO and ethanol
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
PS-48 is a PDK1 activator and binds to the HM/PIF binding pocket rather than the ATP-binding site.
In vitro activity:
The use of 10 µM PS-48 significantly increased the proportion of porcine oocytes reaching the metaphase II (MII) stage and improved cumulus cell expansion. The activation of the PI3K/Akt pathway was associated with the regulation of maturation-related genes and proteins, including Akt, cyclin B1, MOS, BMP15, GDF9, CDC2, mTOR, BAX, BCL2, and caspase-3. Reference: Reprod Domest Anim. 2020 Dec;55(12):1678-1687. https://pubmed.ncbi.nlm.nih.gov/32946622/
In vivo activity:
In a mouse model for Alzheimer's disease, the oral administration of PS-48 was tested to assess its potential in preventing the decline in learning and memory. PS-48 had a positive impact on spatial learning and memory, especially in the mice on a high-fat die. PS-48 modestly reduced body weight and improved glucose tolerance. Reference: Behav Brain Res. 2023 Feb 13;438:114183. https://pubmed.ncbi.nlm.nih.gov/36404570/
Solvent mg/mL mM
Solubility
DMSO 57.0 198.78
Ethanol 57.0 198.78
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 286.75 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Jiao Y, Zhu S, Li J, Jam Zaheer A, Li M, Huang B. PS48 promotes in vitro maturation and developmental competence of porcine oocytes through activating PI3K/Akt signalling pathway. Reprod Domest Anim. 2020 Dec;55(12):1678-1687. doi: 10.1111/rda.13818. Epub 2020 Nov 10. PMID: 32946622. 2. Mordhorst BR, Murphy SL, Ross RM, Benne JA, Samuel MS, Cecil RF, Redel BK, Spate LD, Murphy CN, Wells KD, Green JA, Prather RS. Pharmacologic treatment of donor cells induced to have a Warburg effect-like metabolism does not alter embryonic development in vitro or survival during early gestation when used in somatic cell nuclear transfer in pigs. Mol Reprod Dev. 2018 Apr;85(4):290-302. doi: 10.1002/mrd.22964. Epub 2018 Mar 5. PMID: 29392839; PMCID: PMC5903921. 3. Querfurth H, Slitt A, DiCamillo A, Surles N, DeBoef B, Lee HK. A PDK-1 allosteric agonist improves spatial learning and memory in a βAPP/PS-1 transgenic mouse-high fat diet intervention model of Alzheimer's disease. Behav Brain Res. 2023 Feb 13;438:114183. doi: 10.1016/j.bbr.2022.114183. Epub 2022 Oct 29. PMID: 36404570. 4. Xu WN, Zheng HL, Yang RZ, Jiang LS, Jiang SD. HIF-1α Regulates Glucocorticoid-Induced Osteoporosis Through PDK1/AKT/mTOR Signaling Pathway. Front Endocrinol (Lausanne). 2020 Jan 28;10:922. doi: 10.3389/fendo.2019.00922. PMID: 32047474; PMCID: PMC6997475.
In vitro protocol:
1. Jiao Y, Zhu S, Li J, Jam Zaheer A, Li M, Huang B. PS48 promotes in vitro maturation and developmental competence of porcine oocytes through activating PI3K/Akt signalling pathway. Reprod Domest Anim. 2020 Dec;55(12):1678-1687. doi: 10.1111/rda.13818. Epub 2020 Nov 10. PMID: 32946622. 2. Mordhorst BR, Murphy SL, Ross RM, Benne JA, Samuel MS, Cecil RF, Redel BK, Spate LD, Murphy CN, Wells KD, Green JA, Prather RS. Pharmacologic treatment of donor cells induced to have a Warburg effect-like metabolism does not alter embryonic development in vitro or survival during early gestation when used in somatic cell nuclear transfer in pigs. Mol Reprod Dev. 2018 Apr;85(4):290-302. doi: 10.1002/mrd.22964. Epub 2018 Mar 5. PMID: 29392839; PMCID: PMC5903921.
In vivo protocol:
1. Querfurth H, Slitt A, DiCamillo A, Surles N, DeBoef B, Lee HK. A PDK-1 allosteric agonist improves spatial learning and memory in a βAPP/PS-1 transgenic mouse-high fat diet intervention model of Alzheimer's disease. Behav Brain Res. 2023 Feb 13;438:114183. doi: 10.1016/j.bbr.2022.114183. Epub 2022 Oct 29. PMID: 36404570. 2. Xu WN, Zheng HL, Yang RZ, Jiang LS, Jiang SD. HIF-1α Regulates Glucocorticoid-Induced Osteoporosis Through PDK1/AKT/mTOR Signaling Pathway. Front Endocrinol (Lausanne). 2020 Jan 28;10:922. doi: 10.3389/fendo.2019.00922. PMID: 32047474; PMCID: PMC6997475.
1: Trundle J, Lu-Nguyen N, Malerba A, Popplewell L. Targeted Antisense Oligonucleotide-Mediated Skipping of Murine Postn Exon 17 Partially Addresses Fibrosis in D2.mdx Mice. Int J Mol Sci. 2024 Jun 1;25(11):6113. doi: 10.3390/ijms25116113. PMID: 38892298; PMCID: PMC11172600. 2: Balam S, Miura K, Ayadi I, Konaté D, Incandela NC, Agnolon V, Guindo MA, Diakité SAS, Olugbile S, Nebie I, Herrera SM, Long C, Kajava AV, Diakité M, Corradin G, Herrera S, Herrera MA. Cross-reactivity of rPvs48/45, a recombinant Plasmodium vivax protein, with sera from Plasmodium falciparum endemic areas of Africa. bioRxiv [Preprint]. 2024 Apr 15:2024.04.10.588966. doi: 10.1101/2024.04.10.588966. PMID: 38659832; PMCID: PMC11042229. 3: Trundle J, Cernisova V, Boulinguiez A, Lu-Nguyen N, Malerba A, Popplewell L. Expression of the Pro-Fibrotic Marker Periostin in a Mouse Model of Duchenne Muscular Dystrophy. Biomedicines. 2024 Jan 18;12(1):216. doi: 10.3390/biomedicines12010216. PMID: 38255321; PMCID: PMC10813341. 4: Cernisova V, Lu-Nguyen N, Trundle J, Herath S, Malerba A, Popplewell L. Microdystrophin Gene Addition Significantly Improves Muscle Functionality and Diaphragm Muscle Histopathology in a Fibrotic Mouse Model of Duchenne Muscular Dystrophy. Int J Mol Sci. 2023 May 3;24(9):8174. doi: 10.3390/ijms24098174. PMID: 37175881; PMCID: PMC10179398. 5: Lee LE, Kulnik ST, Curran GM, Boaz A, Ramdharry GM. Protocol for a hybrid II study exploring the feasibility of delivering, evaluating, and implementing a self-management programme for people with neuromuscular diseases at a specialist neuromuscular centre. Pilot Feasibility Stud. 2023 Jan 9;9(1):4. doi: 10.1186/s40814-022-01231-9. PMID: 36624548; PMCID: PMC9827010. 6: Querfurth H, Slitt A, DiCamillo A, Surles N, DeBoef B, Lee HK. A PDK-1 allosteric agonist improves spatial learning and memory in a βAPP/PS-1 transgenic mouse-high fat diet intervention model of Alzheimer's disease. Behav Brain Res. 2023 Feb 13;438:114183. doi: 10.1016/j.bbr.2022.114183. Epub 2022 Oct 29. PMID: 36404570. 7: Luo C, Wang Z, Wang J, Yun F, Lu F, Fu J, Liu Q, Shi D. Individual variation in buffalo somatic cell cloning efficiency is related to glycolytic metabolism. Sci China Life Sci. 2022 Oct;65(10):2076-2092. doi: 10.1007/s11427-021-2039-6. Epub 2022 Mar 25. PMID: 35366153. 8: Querfurth H, Marshall J, Parang K, Rioult-Pedotti MS, Tiwari R, Kwon B, Reisinger S, Lee HK. A PDK-1 allosteric agonist neutralizes insulin signaling derangements and beta-amyloid toxicity in neuronal cells and in vitro. PLoS One. 2022 Jan 21;17(1):e0261696. doi: 10.1371/journal.pone.0261696. Erratum in: PLoS One. 2024 May 29;19(5):e0304731. doi: 10.1371/journal.pone.0304731. PMID: 35061720; PMCID: PMC8782417. 9: Dong W, Zhou R, Chen J, Shu Z, Duan M. Phosphorylation Regulation on the Homo-Dimeric Binding of Transactive Response DNA-Binding Protein. J Chem Inf Model. 2022 Nov 14;62(21):5267-5275. doi: 10.1021/acs.jcim.1c01224. Epub 2022 Jan 18. PMID: 35040651. 10: Choi S, Ferrari G, Tedesco FS. Cellular dynamics of myogenic cell migration: molecular mechanisms and implications for skeletal muscle cell therapies. EMBO Mol Med. 2020 Dec 7;12(12):e12357. doi: 10.15252/emmm.202012357. Epub 2020 Nov 19. PMID: 33210465; PMCID: PMC7721365. 11: Jiao Y, Zhu S, Li J, Jam Zaheer A, Li M, Huang B. PS48 promotes in vitro maturation and developmental competence of porcine oocytes through activating PI3K/Akt signalling pathway. Reprod Domest Anim. 2020 Dec;55(12):1678-1687. doi: 10.1111/rda.13818. Epub 2020 Nov 10. PMID: 32946622. 12: Xu WN, Zheng HL, Yang RZ, Jiang LS, Jiang SD. HIF-1α Regulates Glucocorticoid-Induced Osteoporosis Through PDK1/AKT/mTOR Signaling Pathway. Front Endocrinol (Lausanne). 2020 Jan 28;10:922. doi: 10.3389/fendo.2019.00922. PMID: 32047474; PMCID: PMC6997475. 13: Mordhorst BR, Kerns KC, Schauflinger M, Zigo M, Murphy SL, Ross RM, Wells KD, Green JA, Sutovsky P, Prather RS. Pharmacologic treatment with CPI-613 and PS48 decreases mitochondrial membrane potential and increases quantity of autolysosomes in porcine fibroblasts. Sci Rep. 2019 Jul 1;9(1):9417. doi: 10.1038/s41598-019-45850-4. PMID: 31263141; PMCID: PMC6603033. 14: Liu W, Li P, Mei Y. Discovery of SBF1 as an allosteric inhibitor targeting the PIF-pocket of 3-phosphoinositide-dependent protein kinase-1. J Mol Model. 2019 Jun 13;25(7):187. doi: 10.1007/s00894-019-4069-5. PMID: 31197600. 15: Probst JJ, Martins GHC, Dos Santos Sumar AH, Vieira AM, Bion M, Bobinski F, Horewicz VV, Martins DF, Dos Santos ARS, Bonorino KC, Luiz Dafre A, de Camargo Hizume Kunzler D. Pulmonary and muscle profile in pneumosepsis: A temporal analysis of inflammatory markers. Cytokine. 2019 Feb;114:128-134. doi: 10.1016/j.cyto.2018.11.012. Epub 2018 Nov 20. PMID: 30470659. 16: Mordhorst BR, Murphy SL, Ross RM, Samuel MS, Rojas Salazar S, Ji T, Behura SK, Wells KD, Green JA, Prather RS. Pharmacologic Reprogramming Designed to Induce a Warburg Effect in Porcine Fetal Fibroblasts Alters Gene Expression and Quantities of Metabolites from Conditioned Media Without Increased Cell Proliferation. Cell Reprogram. 2018 Feb;20(1):38-48. doi: 10.1089/cell.2017.0040. PMID: 29412741; PMCID: PMC5804098. 17: Mordhorst BR, Murphy SL, Ross RM, Benne JA, Samuel MS, Cecil RF, Redel BK, Spate LD, Murphy CN, Wells KD, Green JA, Prather RS. Pharmacologic treatment of donor cells induced to have a Warburg effect-like metabolism does not alter embryonic development in vitro or survival during early gestation when used in somatic cell nuclear transfer in pigs. Mol Reprod Dev. 2018 Apr;85(4):290-302. doi: 10.1002/mrd.22964. Epub 2018 Mar 5. PMID: 29392839; PMCID: PMC5903921. 18: Han F, Xue M, Chang Y, Li X, Yang Y, Sun B, Chen L. Triptolide Suppresses Glomerular Mesangial Cell Proliferation in Diabetic Nephropathy Is Associated with Inhibition of PDK1/Akt/mTOR Pathway. Int J Biol Sci. 2017 Sep 21;13(10):1266-1275. doi: 10.7150/ijbs.20485. Erratum in: Int J Biol Sci. 2020 Oct 3;16(15):3037-3038. doi: 10.7150/ijbs.53769. PMID: 29104493; PMCID: PMC5666525. 19: Liu W, Mao J, Xue Y, Zhao Z, Zhang H, Ji X. Nanoparticle Loading Induced Morphological Transitions and Size Fractionation of Coassemblies from PS-b-PAA with Quantum Dots. Langmuir. 2016 Aug 2;32(30):7596-605. doi: 10.1021/acs.langmuir.6b02202. Epub 2016 Jul 22. PMID: 27447738. 20: Watanabe S, Matsumoto T, Oda M, Yamada K, Takagi J, Taguchi K, Kobayashi T. Insulin augments serotonin-induced contraction via activation of the IR/PI3K/PDK1 pathway in the rat carotid artery. Pflugers Arch. 2016 Apr;468(4):667-77. doi: 10.1007/s00424-015-1759-4. Epub 2015 Nov 17. PMID: 26577585.