Wybutine | Wye | CAS#33359-03-4

MedKoo Cat#: 341263 | Name: Wybutine

Description:

WARNING: This product is for research use only, not for human or veterinary use.

Wybutine, also known as YWye or Wye, is a Fluorescent base isolated from Torulopsis utilis. YWye by the size of its aromatic system and by its stacking properties is particularly well suited for stabilizing the native conformation of tRNAPhe.

Chemical Structure

Wybutine

CAS#33359-03-4

Theoretical Analysis

MedKoo Cat#: 341263

Name: Wybutine

CAS#: 33359-03-4

Chemical Formula: C9H9N5O

Exact Mass: 203.0807

Molecular Weight: 203.21

Elemental Analysis: C, 53.20; H, 4.46; N, 34.47; O, 7.87

Price and Availability

This product is currently not in stock but may be available through custom synthesis. To ensure cost efficiency, the minimum order quantity is 1 gram. The estimated lead time is 2 to 4 months, with pricing dependent on the complexity of the synthesis (typically high for intricate chemistries). Quotes for quantities below 1 gram will not be provided. To request a quote, please click the button below. Note: If this product becomes available in stock in the future, pricing will be listed accordingly.

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Related CAS #

No Data

Synonym

Wye; Wybutine; Y Base; YWye,

IUPAC/Chemical Name

9H-Imidazo(1,2-a)purin-9-one, 1,4-dihydro-4,6-dimethyl-

InChi Key

ZLHLYESIHSHXGM-UHFFFAOYSA-N

InChi Code

InChI=1S/C9H9N5O/c1-5-3-14-8(15)6-7(11-4-10-6)13(2)9(14)12-5/h3-4H,1-2H3,(H,10,11)

SMILES Code

O=C1N2C(N(C)C3=C1NC=N3)=NC(C)=C2

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Instruction

View handling instruction

Preparing Stock Solutions

The following data is based on the product molecular weight 203.21 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

1: Kumbhar NM, Sonawane KD. Iso-energetic multiple conformations of hypermodified nucleic acid base wybutine (yW) which occur at 37(th) position in anticodon loop of tRNA(Phe). J Mol Graph Model. 2011 Jun;29(7):935-46. doi: 10.1016/j.jmgm.2011.03.005. Epub 2011 Apr 12. PubMed PMID: 21530341. 2: Claesens F, Rigler R. Conformational dynamics of the anticodon loop in yeast tRNAPhe as sensed by the fluorescence of wybutine. Eur Biophys J. 1986;13(6):331-42. PubMed PMID: 3530734. 3: Paulsen H, Robertson JM, Wintermeyer W. Effect of ribosome binding and translocation on the anticodon of tRNAPhe as studied by wybutine fluorescence. Nucleic Acids Res. 1982 Apr 24;10(8):2651-63. PubMed PMID: 7043399; PubMed Central PMCID: PMC320640. 4: Ehrlich R, Lefevre JF, Remy P. Fluorimetric study of the complex between yeast phenylalanyl-tRNA synthetase and tRNA-Phe. 1. Changes in the conformation of the enzyme and tRNA; modification of the Wybutine neighbourhood. Eur J Biochem. 1980 Jan;103(1):145-53. PubMed PMID: 6987055. 5: Wintermeyer W, Schleich HG, Zachau HG. Incorporation of amines or hydrazines into tRNA replacing wybutine or dihydrouracil. Methods Enzymol. 1979;59:110-21. PubMed PMID: 440077. 6: Schleich HG, Wintermeyer W, Zachau HG. Replacement of wybutine by hydrazines and its effect on the active conformation of yeast tRNAPhe. Nucleic Acids Res. 1978 May;5(5):1701-13. PubMed PMID: 351568; PubMed Central PMCID: PMC342114.