ROCK-IN-D1 | CAS#1219721-73-9 | ROCK inhibitor

MedKoo Cat#: 562374 | Name: ROCK-IN-D1

Description:

WARNING: This product is for research use only, not for human or veterinary use.

ROCK-IN-D1 is a potent and selective ROCK inhibitor.

Chemical Structure

ROCK-IN-D1

CAS#1219721-73-9

Theoretical Analysis

MedKoo Cat#: 562374

Name: ROCK-IN-D1

CAS#: 1219721-73-9

Chemical Formula: C21H25N5OS

Exact Mass: 395.1780

Molecular Weight: 395.52

Elemental Analysis: C, 63.77; H, 6.37; N, 17.71; O, 4.05; S, 8.11

Price and Availability

This product is currently not in stock but may be available through custom synthesis. To ensure cost efficiency, the minimum order quantity is 1 gram. The estimated lead time is 2 to 4 months, with pricing dependent on the complexity of the synthesis (typically high for intricate chemistries). Quotes for quantities below 1 gram will not be provided. To request a quote, please click the button below. Note: If this product becomes available in stock in the future, pricing will be listed accordingly.

Bulk Inquiry

Related CAS #

No Data

Synonym

ROCK-IN-D1; ROCK IN D1; ROCKIND1; ROCK inhibitor D1; ROCK-inhibitor-D1;

IUPAC/Chemical Name

1-Benzyl-3-[2-(2-dimethylamino-ethylsulfanyl)-4-(1H-pyrazol-4-yl)-phenyl]-urea

InChi Key

RWHYCJQLNMIKGT-UHFFFAOYSA-N

InChi Code

InChI=1S/C21H25N5OS/c1-26(2)10-11-28-20-12-17(18-14-23-24-15-18)8-9-19(20)25-21(27)22-13-16-6-4-3-5-7-16/h3-9,12,14-15H,10-11,13H2,1-2H3,(H,23,24)(H2,22,25,27)

SMILES Code

O=C(NC1=CC=C(C2=CNN=C2)C=C1SCCN(C)C)NCC3=CC=CC=C3

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Instruction

View handling instruction

Preparing Stock Solutions

The following data is based on the product molecular weight 395.52 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

1: Wei J, Huang Q, Bai F, Lin J, Nie J, Lu S, Lu C, Huang R, Lu Z, Lin X. Didymin induces apoptosis through mitochondrial dysfunction and up-regulation of RKIP in human hepatoma cells. Chem Biol Interact. 2017 Jan 5;261:118-126. doi: 10.1016/j.cbi.2016.11.026. Epub 2016 Nov 27. PubMed PMID: 27899290. 2: Qiao F, Zou Z, Liu C, Zhu X, Wang X, Yang C, Jiang T, Chen Y. ROCK2 mediates the proliferation of pulmonary arterial endothelial cells induced by hypoxia in the development of pulmonary arterial hypertension. Exp Ther Med. 2016 Jun;11(6):2567-2572. Epub 2016 Mar 31. PubMed PMID: 27284349; PubMed Central PMCID: PMC4887771. 3: Jiang C, Huang H, Liu J, Wang Y, Zhao Y, Xu Z. [Effects of fasudil on bleomycin-induced pulmonary fibrosis in mice and on the biological behaviors in NIH3T3 mouse fibroblast cell line]. Zhonghua Jie He He Hu Xi Za Zhi. 2014 Sep;37(9):671-6. Chinese. PubMed PMID: 25533689. 4: Shao Q, Kannan A, Lin Z, Stack BC Jr, Suen JY, Gao L. BET protein inhibitor JQ1 attenuates Myc-amplified MCC tumor growth in vivo. Cancer Res. 2014 Dec 1;74(23):7090-102. doi: 10.1158/0008-5472.CAN-14-0305. Epub 2014 Oct 2. PubMed PMID: 25277525; PubMed Central PMCID: PMC4322674. 5: Kim JE, Ryu HJ, Kim MJ, Kang TC. LIM kinase-2 induces programmed necrotic neuronal death via dysfunction of DRP1-mediated mitochondrial fission. Cell Death Differ. 2014 Jul;21(7):1036-49. doi: 10.1038/cdd.2014.17. Epub 2014 Feb 21. PubMed PMID: 24561342; PubMed Central PMCID: PMC4207472. 6: Zhao Y, Lv M, Lin H, Cui Y, Wei X, Qin Y, Kohama K, Gao Y. Rho-associated protein kinase isoforms stimulate proliferation of vascular smooth muscle cells through ERK and induction of cyclin D1 and PCNA. Biochem Biophys Res Commun. 2013 Mar 15;432(3):488-93. doi: 10.1016/j.bbrc.2013.02.009. Epub 2013 Feb 10. PubMed PMID: 23402758. 7: Boku S, Nakagawa S, Toda H, Kato A, Takamura N, Omiya Y, Inoue T, Koyama T. ROCK2 regulates bFGF-induced proliferation of SH-SY5Y cells through GSK-3β and β-catenin pathway. Brain Res. 2013 Jan 25;1492:7-17. doi: 10.1016/j.brainres.2012.11.034. Epub 2012 Dec 2. PubMed PMID: 23211630. 8: Duvvuri U, Shiwarski DJ, Xiao D, Bertrand C, Huang X, Edinger RS, Rock JR, Harfe BD, Henson BJ, Kunzelmann K, Schreiber R, Seethala RS, Egloff AM, Chen X, Lui VW, Grandis JR, Gollin SM. TMEM16A induces MAPK and contributes directly to tumorigenesis and cancer progression. Cancer Res. 2012 Jul 1;72(13):3270-81. doi: 10.1158/0008-5472.CAN-12-0475-T. Epub 2012 May 7. PubMed PMID: 22564524; PubMed Central PMCID: PMC3694774. 9: Choi HJ, Chang BJ, Han JS. Phospholipase D1 is an important regulator of bFGF-induced neurotrophin-3 expression and neurite outgrowth in H19-7 cells. Mol Neurobiol. 2012 Jun;45(3):507-19. doi: 10.1007/s12035-012-8268-7. Epub 2012 Apr 28. PubMed PMID: 22544632. 10: Choi HJ, Han JS. Overexpression of phospholipase D enhances Bcl-2 expression by activating STAT3 through independent activation of ERK and p38MAPK in HeLa cells. Biochim Biophys Acta. 2012 Jun;1823(6):1082-91. doi: 10.1016/j.bbamcr.2012.03.015. Epub 2012 Apr 5. PubMed PMID: 22504301. 11: Suzuki H, Yamamoto T, Fujigaki Y, Eguchi S, Hishida A. Comparison of ROCK and EGFR activation pathways in the progression of glomerular injuries in AngII-infused rats. Ren Fail. 2011;33(10):1005-12. doi: 10.3109/0886022X.2011.618923. PubMed PMID: 22013934. 12: Zhong WB, Hsu SP, Ho PY, Liang YC, Chang TC, Lee WS. Lovastatin inhibits proliferation of anaplastic thyroid cancer cells through up-regulation of p27 by interfering with the Rho/ROCK-mediated pathway. Biochem Pharmacol. 2011 Dec 1;82(11):1663-72. doi: 10.1016/j.bcp.2011.08.021. Epub 2011 Sep 2. PubMed PMID: 21907187. 13: Santos A, Bakker AD, de Blieck-Hogervorst JM, Klein-Nulend J. WNT5A induces osteogenic differentiation of human adipose stem cells via rho-associated kinase ROCK. Cytotherapy. 2010 Nov;12(7):924-32. doi: 10.3109/14653241003774011. PubMed PMID: 20429785. 14: Kim H, Kang M, Lee SA, Kwak TK, Jung O, Lee HJ, Kim SH, Lee JW. TM4SF5 accelerates G1/S phase progression via cytosolic p27Kip1 expression and RhoA activity. Biochim Biophys Acta. 2010 Aug;1803(8):975-82. doi: 10.1016/j.bbamcr.2010.04.001. Epub 2010 Apr 22. PubMed PMID: 20399237. 15: Ordóñez-Morán P, Alvarez-Díaz S, Valle N, Larriba MJ, Bonilla F, Muñoz A. The effects of 1,25-dihydroxyvitamin D3 on colon cancer cells depend on RhoA-ROCK-p38MAPK-MSK signaling. J Steroid Biochem Mol Biol. 2010 Jul;121(1-2):355-61. doi: 10.1016/j.jsbmb.2010.02.031. Epub 2010 Mar 17. PubMed PMID: 20223287. 16: Larrea MD, Hong F, Wander SA, da Silva TG, Helfman D, Lannigan D, Smith JA, Slingerland JM. RSK1 drives p27Kip1 phosphorylation at T198 to promote RhoA inhibition and increase cell motility. Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9268-73. doi: 10.1073/pnas.0805057106. Epub 2009 May 22. PubMed PMID: 19470470; PubMed Central PMCID: PMC2695095. 17: Li Z, Wang C, Jiao X, Katiyar S, Casimiro MC, Prendergast GC, Powell MJ, Pestell RG. Alternate cyclin D1 mRNA splicing modulates p27KIP1 binding and cell migration. J Biol Chem. 2008 Mar 14;283(11):7007-15. doi: 10.1074/jbc.M706992200. Epub 2008 Jan 7. PubMed PMID: 18180298. 18: Chen J, Guerriero E, Lathrop K, SundarRaj N. Rho/ROCK signaling in regulation of corneal epithelial cell cycle progression. Invest Ophthalmol Vis Sci. 2008 Jan;49(1):175-83. doi: 10.1167/iovs.07-0488. PubMed PMID: 18172090. 19: Pacary E, Tixier E, Coulet F, Roussel S, Petit E, Bernaudin M. Crosstalk between HIF-1 and ROCK pathways in neuronal differentiation of mesenchymal stem cells, neurospheres and in PC12 neurite outgrowth. Mol Cell Neurosci. 2007 Jul;35(3):409-23. Epub 2007 Apr 10. PubMed PMID: 17493827. 20: Yu S, Fang N, Li Q, Zhang J, Luo H, Ronis M, Badger TM. In vitro actions on human cancer cells and the liquid chromatography-mass spectrometry/mass spectrometry fingerprint of phytochemicals in rice protein isolate. J Agric Food Chem. 2006 Jun 14;54(12):4482-92. PubMed PMID: 16756384.