Synonym
Diazobenzenesulfonic acid; DASA; Diazobenzene sulfonate; Diazobenzenesulfonate; Diazobenzenesulphonate; diazobenzene sulfonic acid;
IUPAC/Chemical Name
4-Sulfobenzenediazonium
InChi Key
UEUIKXVPXLWUDU-UHFFFAOYSA-O
InChi Code
InChI=1S/C6H4N2O3S/c7-8-5-1-3-6(4-2-5)12(9,10)11/h1-4H/p+1
SMILES Code
N#[N+]C1=CC=C(S(=O)(O)=O)C=C1
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
Preparing Stock Solutions
The following data is based on the
product
molecular weight
185.18
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
1: FRANCA F. [Effects of substances activating the diazo reaction and of diazobenzenesulfonic acid (diazoreactive) on natural bonds of bilirubin in organic fluids]. Acta Med Patav. 1955 Jan-Mar;15(1):69-76. Italian. PubMed PMID: 13394143.
2: Maksay G, Ticku MK. Characterization of gamma-aminobutyric acid-benzodiazepine receptor complexes by protection against inactivation by group-specific reagents. J Neurochem. 1984 Jun;42(6):1715-27. PubMed PMID: 6327911.
3: Kasai H, Takahashi K, Ando T. Chemical modification of tyrosine residues in ribonuclease T1 with N-acetylimidazole and p-diazobenzenesulfonic acid. J Biochem. 1977 Jun;81(6):1751-8. PubMed PMID: 408339.
4: STEINITZ K. [A simplified determination of serum bilirubin with diazobenzenesulfonic acid]. Harefuah. 1952 Jul 15;43(1-2):13. Undetermined Language. PubMed PMID: 13021645.
5: Nishimoto SK, Zhao J, Dass C. Isolation and characterization of the reaction product of 4-diazobenzenesulfonic acid and gamma-carboxyglutamic acid: modification of the assay for measurement of beta-carboxyaspartic acid. Anal Biochem. 1994 Jan;216(1):159-64. PubMed PMID: 8135347.
6: Landis JB, Pardue HL. Kinetics of the reactions of unconjugated and conjugated bilirubins with p-diazobenzenesulfonic acid. Clin Chem. 1978 Oct;24(10):1690-9. PubMed PMID: 29720.
7: Pfleiderer G, Jeckel D. [Coupling reaction of lactate dehydrogenase after reversible masking of the SH-groups. Reaction with p-diazobenzenesulfonic acid for marking essential histidine or tyrosine residues]. Eur J Biochem. 1967 Sep;2(2):171-5. German. PubMed PMID: 6078529.
8: Maksay G, Ticku MK. Diazotization and thiocyanate differentiate agonists from antagonists for the high- and low-affinity receptors of gamma-aminobutyric acid. J Neurochem. 1984 Jul;43(1):261-8. PubMed PMID: 6327917.
9: Nishimoto SK. A colorimetric assay specific for gamma-carboxyglutamic acid-containing proteins: its utility in protein purification procedures. Anal Biochem. 1990 May 1;186(2):273-9. PubMed PMID: 2363499.
10: Jie KS, Gijsbers BL, Vermeer C. A specific colorimetric staining method for gamma-carboxyglutamic acid-containing proteins in polyacrylamide gels. Anal Biochem. 1995 Jan 1;224(1):163-5. PubMed PMID: 7535983.
11: Kabasawa I, Murayama K, Kimura M, Sekine T. Thiols of myosin. II. Chemical modification of sulfhydryl groups of myosin A adenosine triphosphatase with diazobenzenesulfonic acid. J Biochem. 1974 Jun;75(6):1275-81. PubMed PMID: 4279252.
12: Artman M, Seeley RJ. Studies on the sidedness of the human red blood cell membrane: preparation of stable azo-erythrocytes. Prep Biochem. 1981;11(3):291-8. PubMed PMID: 6265896.
13: Shimada Y, Shinmyo A, Enatsu T. Purification and properties of one component of acid phosphatase produced by Aspergillus niger. Biochim Biophys Acta. 1977 Feb 9;480(2):417-27. PubMed PMID: 13843.
14: Bouchet MJ, Jacques P, Ilien B, Goeldner M, Hirth C. m-Sulfonate benzene diazonium chloride: a powerful affinity label for the gamma-aminobutyric acid binding site from rat brain. J Neurochem. 1992 Oct;59(4):1405-13. PubMed PMID: 1402891.
15: Burch TP, Thyagarajan R, Ticku MK. Group-selective reagent modification of the benzodiazepine-gamma-aminobutyric acid receptor-ionophore complex reveals that low-affinity gamma-aminobutyric acid receptors stimulate benzodiazepine binding. Mol Pharmacol. 1983 Jan;23(1):52-9. PubMed PMID: 6306426.
16: Goldstein IM, Cerqueira M, Lind S, Kaplan HB. Evidence that the superoxide-generating system of human leukocytes is associated with the cell surface. J Clin Invest. 1977 Feb;59(2):249-54. PubMed PMID: 188867; PubMed Central PMCID: PMC333354.
17: Ohno Y, Hirai K, Kanoh T, Uchino H, Ogawa K. Subcellular localization of H2O2 production in human neutrophils stimulated with particles and an effect of cytochalasin-B on the cells. Blood. 1982 Jul;60(1):253-60. PubMed PMID: 7082841.
18: BERKA A, ZYKA J. [Copulation reaction with p-diazobenzenesulphonic acid. I. Photometric determination of some drugs]. Cesk Farm. 1955 Jun;4(5):225-7. Czech. PubMed PMID: 14390578.
19: JINDRA A, KALVODA R, ZYKA J. [Polarometric determination of certain pharmaceutically important substances with para-diazobenzenesulphonic acid]. Cas Cesk Lek. 1950;63(7-8):106-10. Undetermined Language. PubMed PMID: 14792571.
20: McPhail LC, Henson PM, Johnston RB Jr. Respiratory burst enzyme in human neutrophils. Evidence for multiple mechanisms of activation. J Clin Invest. 1981 Mar;67(3):710-6. PubMed PMID: 6259208; PubMed Central PMCID: PMC370621.