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MedKoo Cat#: 527785 | Name: NSC-144303
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

NSC-144303 is a potent inhibitor of general DUB activity leading to acute proteotoxicstress and apoptosis.

Chemical Structure

NSC-144303
NSC-144303
CAS#108477-18-5

Theoretical Analysis

MedKoo Cat#: 527785

Name: NSC-144303

CAS#: 108477-18-5

Chemical Formula: C19H14N2O7S

Exact Mass: 414.0522

Molecular Weight: 414.39

Elemental Analysis: C, 55.07; H, 3.41; N, 6.76; O, 27.03; S, 7.74

Price and Availability

Size Price Availability Quantity
10mg USD 360.00
50mg USD 1,205.00
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Related CAS #
No Data
Synonym
NSC-144303; NSC144303; NSC 144303; G5
IUPAC/Chemical Name
Tetrahydro-3,5-bis[(4-nitrophenyl)methylene]-4H-thiopyran-4-one 1,1-dioxide
InChi Key
SSAMIOWVUGBIJK-KAVGSWPWSA-N
InChi Code
InChI=1S/C19H14N2O7S/c22-19-15(9-13-1-5-17(6-2-13)20(23)24)11-29(27,28)12-16(19)10-14-3-7-18(8-4-14)21(25)26/h1-10H,11-12H2/b15-9+,16-10+
SMILES Code
O=C(/C(C1)=C/C2=CC=C([N+]([O-])=O)C=C2)/C(CS1(=O)=O)=C/C3=CC=C([N+]([O-])=O)C=C3
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
Ubiquitin Isopeptidase Inhibitor I, G5 (NSC 144303) is an apoptosome-independent caspase and apoptosis activator with IC50 values of 1.76 and 1.6 μM in E1A and E1A/C9DN cells.
In vitro activity:
Dose-dependent studies indicated that G5 induces 70% of death in WT cells when used at a 2.5 μm concentration (Fig. 1A). In DKO cells, treated with the same concentration of G5, death occurred in less than 20% of cells. However, doubling the concentration (5 μm) was enough to induce 50% of death in DKO cells. Cell death of DKO cells was further increased up to 80% in the presence of 10 μm G5 (Fig. 1A). Reference: J Biol Chem. 2009 Mar 27;284(13):8369-81. https://pubmed.ncbi.nlm.nih.gov/19139105/
In vivo activity:
TBD
Solvent mg/mL mM
Solubility
DMSO 28.6 68.95
DMSO:PBS (pH 7.2) (1:3) 0.3 0.60
THF 3.4 8.30
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 414.39 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Fontanini A, Foti C, Potu H, Crivellato E, Maestro R, Bernardi P, Demarchi F, Brancolini C. The Isopeptidase Inhibitor G5 Triggers a Caspase-independent Necrotic Death in Cells Resistant to Apoptosis: A COMPARATIVE STUDY WITH THE PROTEASOME INHIBITOR BORTEZOMIB. J Biol Chem. 2009 Mar 27;284(13):8369-81. doi: 10.1074/jbc.M806113200. Epub 2009 Jan 12. PMID: 19139105; PMCID: PMC2659195. 2. Aleo E, Henderson CJ, Fontanini A, Solazzo B, Brancolini C. Identification of new compounds that trigger apoptosome-independent caspase activation and apoptosis. Cancer Res. 2006 Sep 15;66(18):9235-44. doi: 10.1158/0008-5472.CAN-06-0702. PMID: 16982768.
In vitro protocol:
1. Fontanini A, Foti C, Potu H, Crivellato E, Maestro R, Bernardi P, Demarchi F, Brancolini C. The Isopeptidase Inhibitor G5 Triggers a Caspase-independent Necrotic Death in Cells Resistant to Apoptosis: A COMPARATIVE STUDY WITH THE PROTEASOME INHIBITOR BORTEZOMIB. J Biol Chem. 2009 Mar 27;284(13):8369-81. doi: 10.1074/jbc.M806113200. Epub 2009 Jan 12. PMID: 19139105; PMCID: PMC2659195. 2. Aleo E, Henderson CJ, Fontanini A, Solazzo B, Brancolini C. Identification of new compounds that trigger apoptosome-independent caspase activation and apoptosis. Cancer Res. 2006 Sep 15;66(18):9235-44. doi: 10.1158/0008-5472.CAN-06-0702. PMID: 16982768.
In vivo protocol:
TBD
1: Aleo E, Henderson CJ, Fontanini A, Solazzo B, Brancolini C. Identification of new compounds that trigger apoptosome-independent caspase activation and apoptosis. Cancer Res. 2006 Sep 15;66(18):9235-44. PubMed PMID: 16982768.