MedKoo Cat#: 561879 | Name: FM4-64

Description:

WARNING: This product is for research use only, not for human or veterinary use.

FM4-64 is a fluorescent probe used for monitoring synaptic activity at neuromuscular junctions.

Chemical Structure

FM4-64
FM4-64
CAS#162112-35-8

Theoretical Analysis

MedKoo Cat#: 561879

Name: FM4-64

CAS#: 162112-35-8

Chemical Formula: C30H45Br2N3

Exact Mass:

Molecular Weight: 607.51

Elemental Analysis: C, 59.31; H, 7.47; Br, 26.31; N, 6.92

Price and Availability

This product is currently not in stock but may be available through custom synthesis. To ensure cost efficiency, the minimum order quantity is 1 gram. The estimated lead time is 2 to 4 months, with pricing dependent on the complexity of the synthesis (typically high for intricate chemistries). Quotes for quantities below 1 gram will not be provided. To request a quote, please click the button below. Note: If this product becomes available in stock in the future, pricing will be listed accordingly.
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Synonym
FM4-64; FM464; FM4 64; Frie Mao 4-64; SynaptoRed Reagent; SynaptoRed C2;
IUPAC/Chemical Name
4-((1E,3E,5E)-6-(4-(diethylamino)phenyl)hexa-1,3,5-trien-1-yl)-1-(3-(triethylammonio)propyl)pyridin-1-ium bromide
InChi Key
AFVSZGYRRUMOFH-UHFFFAOYSA-L
InChi Code
InChI=1S/C30H45N3.2BrH/c1-6-32(7-2)30-20-18-28(19-21-30)16-13-11-12-14-17-29-22-25-31(26-23-29)24-15-27-33(8-3,9-4)10-5;;/h11-14,16-23,25-26H,6-10,15,24,27H2,1-5H3;2*1H/q+2;;/p-2
SMILES Code
CCN(CC)C1=CC=C(/C=C/C=C/C=C/C2=CC=[N+](CCC[N+](CC)(CC)CC)C=C2)C=C1.[Br-].[Br-]
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info

Preparing Stock Solutions

The following data is based on the product molecular weight 607.51 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1: Takeda A, Tamano H. Significance of Low Nanomolar Concentration of Zn(2+) in Artificial Cerebrospinal Fluid. Mol Neurobiol. 2017 May;54(4):2477-2482. doi: 10.1007/s12035-016-9816-3. Epub 2016 Mar 16. Review. PubMed PMID: 26984599. 2: McKinney RA. Physiological roles of spine motility: development, plasticity and disorders. Biochem Soc Trans. 2005 Dec;33(Pt 6):1299-302. Review. PubMed PMID: 16246103. 3: Meckel T, Hurst AC, Thiel G, Homann U. Guard cells undergo constitutive and pressure-driven membrane turnover. Protoplasma. 2005 Oct;226(1-2):23-9. Epub 2005 Oct 20. Review. PubMed PMID: 16231098. 4: Murphy AS, Bandyopadhyay A, Holstein SE, Peer WA. Endocytotic cycling of PM proteins. Annu Rev Plant Biol. 2005;56:221-51. Review. PubMed PMID: 15862095. 5: Bolte S, Talbot C, Boutte Y, Catrice O, Read ND, Satiat-Jeunemaitre B. FM-dyes as experimental probes for dissecting vesicle trafficking in living plant cells. J Microsc. 2004 May;214(Pt 2):159-73. Review. PubMed PMID: 15102063.