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MedKoo Cat#: 561715 | Name: PMSF
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

PMSF is an inhibitor of serine proteases such as trypsin and chymotrypsin. It also inhibits cysteine proteases (reversible by reduced thiols) and mammalian acetylcholinesterase.

Chemical Structure

PMSF
PMSF
CAS#329-98-6

Theoretical Analysis

MedKoo Cat#: 561715

Name: PMSF

CAS#: 329-98-6

Chemical Formula: C7H7FO2S

Exact Mass: 174.0151

Molecular Weight: 174.19

Elemental Analysis: C, 48.27; H, 4.05; F, 10.91; O, 18.37; S, 18.41

Price and Availability

Size Price Availability Quantity
5g USD 180.00
25g USD 340.00
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Synonym
PMSF; Phenylmethylsulfonyl fluoride; Benzylsulfonyl fluoride;
IUPAC/Chemical Name
Benzylsulfonyl fluoride
InChi Key
YBYRMVIVWMBXKQ-UHFFFAOYSA-N
InChi Code
InChI=1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2
SMILES Code
O=S(CC1=CC=CC=C1)(F)=O
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
PMSF is an irreversible serine/cysteine protease inhibitor commonly used in the preparation of cell lysates.
In vitro activity:
Phenylmethanesulfonyl fluoride (PMSF) (2 mM), a putative inhibitor of phosphatidylinositol-specific phospholipase C, almost completely inhibited carbachol-stimulated inositol incorporation into phosphatidylinositol (PI) of longitudinal smooth muscle of guinea pig ileum, while it had no effect on potassium-stimulated inositol incorporation. This suggests that the two stimuli may affect phosphoinositide turnover by different mechanisms, distinguishable by PMSF. In contrast to its specific inhibition of carbachol-stimulated phosphoinositide turnover, PMSF produced a transient inhibition of contraction by both carbachol and potassium. The non-selective effect of PMSF on contraction suggests that it is not the result of its inhibitory effect on phosphoinositide breakdown. PMSF (2 mM) inhibited carbachol-stimulated inositol phosphate accumulation in the presence of Li+ by only 15%-19%, indicating that PMSF inhibition of phosphoinositide turnover was not due to its inhibition of phosphoinositide phosphodiesterase, but to one or more steps following phosphoinositide breakdown. Reference: Cell Calcium. 1984 Jun;5(3):191-203. https://pubmed.ncbi.nlm.nih.gov/6090026/
In vivo activity:
Mice receiving i.p. injections of PMSF exhibited cannabinoid effects that included antinociception, hypothermia and immobility with ED50 values of 86, 224 and 206 mg/kg, respectively. Spontaneous activity was reduced at doses greater than 100 mg/kg. However, none of these effects was blocked by the cannabinoid antagonist SR 141716A. On the other hand, pretreatment with an inactive dose of PMSF (30 mg/kg) potentiated the effects of anandamide on tail-flick response (antinociception), spontaneous activity and mobility by 5-, 10- and 8-fold, respectively. PMSF did not alter anandamide's hypothermic effects. Overall, these findings with PMSF underscore the importance of metabolism in the actions of anandamide. Reference: J Pharmacol Exp Ther. 1997 Dec;283(3):1138-43. https://pubmed.ncbi.nlm.nih.gov/9399986/
Solvent mg/mL mM comments
Solubility
DMF 33.0 189.45
DMF:PBS (pH 7.2) (1:1) 0.5 2.87
DMSO 95.8 550.01
Ethanol 18.7 107.41
Isopropanol 60.0 344.45
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 174.19 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Güther ML, Masterson WJ, Ferguson MA. The effects of phenylmethylsulfonyl fluoride on inositol-acylation and fatty acid remodeling in African trypanosomes. J Biol Chem. 1994 Jul 15;269(28):18694-701. PMID: 7518442. 2. Sekar MC, Roufogalis BD. Differential effects of phenylmethanesulfonyl fluoride (PMSF) on carbachol and potassium stimulated phosphoinositide turnover and contraction in longitudinal smooth muscle of guinea pig ileum. Cell Calcium. 1984 Jun;5(3):191-203. doi: 10.1016/0143-4160(84)90035-6. PMID: 6090026. 3. Compton DR, Martin BR. The effect of the enzyme inhibitor phenylmethylsulfonyl fluoride on the pharmacological effect of anandamide in the mouse model of cannabimimetic activity. J Pharmacol Exp Ther. 1997 Dec;283(3):1138-43. PMID: 9399986. 4. Pinsky C, Dua AK, LaBella FS. Phenylmethylsulfonyl fluoride (PMSF) given systemically produces naloxone-reversible analgesia and potentiates effects of beta-endorphin given centrally. Life Sci. 1982 Sep 20-27;31(12-13):1193-6. doi: 10.1016/0024-3205(82)90340-x. PMID: 6292607.
In vitro protocol:
1. Güther ML, Masterson WJ, Ferguson MA. The effects of phenylmethylsulfonyl fluoride on inositol-acylation and fatty acid remodeling in African trypanosomes. J Biol Chem. 1994 Jul 15;269(28):18694-701. PMID: 7518442. 2. Sekar MC, Roufogalis BD. Differential effects of phenylmethanesulfonyl fluoride (PMSF) on carbachol and potassium stimulated phosphoinositide turnover and contraction in longitudinal smooth muscle of guinea pig ileum. Cell Calcium. 1984 Jun;5(3):191-203. doi: 10.1016/0143-4160(84)90035-6. PMID: 6090026.
In vivo protocol:
1. Compton DR, Martin BR. The effect of the enzyme inhibitor phenylmethylsulfonyl fluoride on the pharmacological effect of anandamide in the mouse model of cannabimimetic activity. J Pharmacol Exp Ther. 1997 Dec;283(3):1138-43. PMID: 9399986. 2. Pinsky C, Dua AK, LaBella FS. Phenylmethylsulfonyl fluoride (PMSF) given systemically produces naloxone-reversible analgesia and potentiates effects of beta-endorphin given centrally. Life Sci. 1982 Sep 20-27;31(12-13):1193-6. doi: 10.1016/0024-3205(82)90340-x. PMID: 6292607.
1: Keestra AM, de Zoete MR, Bouwman LI, Vaezirad MM, van Putten JP. Unique features of chicken Toll-like receptors. Dev Comp Immunol. 2013 Nov;41(3):316-23. doi: 10.1016/j.dci.2013.04.009. Epub 2013 Apr 28. Review. PubMed PMID: 23628643. 2: Kudo Y. [Binding Characterizations of Asp-hemolysin to Oxidized Low-Density Lipoprotein]. Yakugaku Zasshi. 2005 Aug;125(8):617-29. Review. Japanese. PubMed PMID: 16079612. 3: Pope CN, Tanaka D Jr, Padilla S. The role of neurotoxic esterase (NTE) in the prevention and potentiation of organophosphorus-induced delayed neurotoxicity (OPIDN). Chem Biol Interact. 1993 Jun;87(1-3):395-406. Review. PubMed PMID: 8343996. 4: Derkx FH, van den Meiracker AH, Fischli W, Admiraal PJ, Man in't Veld AJ, van Brummelen P, Schalekamp MA. Nonparallel effects of renin inhibitor treatment on plasma renin activity and angiotensins I and II in hypertensive subjects. An assay-related artifact. Am J Hypertens. 1991 Jul;4(7 Pt 1):602-9. Review. PubMed PMID: 1873015. 5: Carrington CD. Prophylaxis and the mechanism for the initiation of organophosphorous compound-induced delayed neurotoxicity. Arch Toxicol. 1989;63(3):165-72. Review. PubMed PMID: 2669680. 6: Hubbard JR, Kalimi M. Influence of proteinase inhibitors on glucocorticoid receptor properties: recent progress and future perspectives. Mol Cell Biochem. 1985 Mar;66(2):101-9. Review. PubMed PMID: 3885003. 7: Pringle JR. Methods for avoiding proteolytic artefacts in studies of enzymes and other proteins from yeasts. Methods Cell Biol. 1975;12:149-84. Review. PubMed PMID: 589.