IUPAC/Chemical Name
Methyl 6-butyl-4-oxo-7-phenylmethoxy-1H-quinoline-3-carboxylate
InChi Key
NNOPDLNHPOLRRE-UHFFFAOYSA-N
InChi Code
InChI=1S/C22H23NO4/c1-3-4-10-16-11-17-19(23-13-18(21(17)24)22(25)26-2)12-20(16)27-14-15-8-6-5-7-9-15/h5-9,11-13H,3-4,10,14H2,1-2H3,(H,23,24)
SMILES Code
O=C(C1=CNC2=C(C=C(CCCC)C(OCC3=CC=CC=C3)=C2)C1=O)OC
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
Biological target:
Nequinate, a quinoline compound, is an anticoccidial agent against cecal coccidiosis (Eimeria tenella) infections.
Preparing Stock Solutions
The following data is based on the
product
molecular weight
365.43
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
| 1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
| 5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
| 10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
| 50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
Formulation protocol:
TBD
1: Clarke L, Moloney M, O'Mahony J, O'Kennedy R, Danaher M. Determination of 20 coccidiostats in milk, duck muscle and non-avian muscle tissue using UHPLC-MS/MS. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2013;30(6):958-69. doi: 10.1080/19440049.2013.794306. Epub 2013 Jun 3. PubMed PMID: 23731083.
2: Nakajima T, Hayashi H, Sasamoto T, Kanda M, Kusano T, Matsushima Y, Kanai S, Takeba K, Nagayama T. [Determination of nequinate and buquinolate in livestock products using liquid chromatography-tandem mass spectrometry]. Shokuhin Eiseigaku Zasshi. 2011;52(3):178-82. PubMed PMID: 21720123.
3: Dousa M, Lemr K, Stránský Z. Determination of methylbenzoquate in premixes and animal feeds using liquid chromatography with fluorescence detection. J AOAC Int. 2005 May-Jun;88(3):673-8. PubMed PMID: 16001838.
4: Daugschies A, Gässlein U, Rommel M. Comparative efficacy of anticoccidials under the conditions of commercial broiler production and in battery trials. Vet Parasitol. 1998 Apr 15;76(3):163-71. PubMed PMID: 9615950.
5: Chapman HD. Eimeria tenella, E. acervulina and E. maxima: studies on the development of resistance to diclazuril and other anticoccidial drugs in the chicken. Parasitology. 1989 Oct;99 Pt 2:189-92. PubMed PMID: 2556680.
6: Chapman HD. Sensitivity of field isolates of Eimeria tenella to anticoccidial drugs in the chicken. Res Vet Sci. 1989 Jul;47(1):125-8. PubMed PMID: 2549593.
7: Merson GH, Hill LA, Johnson SF. Determination of methyl benzoquate in poultry feedingstuffs using high-performance liquid chromatography. Analyst. 1985 Jul;110(7):761-4. PubMed PMID: 3880516.
8: Varewyck H, Peeters JE, Halen P, Lampo P, Bouquet Y. Influence of the anticoccidials clopidol/methylbenzoquate and robenidine on fertility and progeny of female rabbits. Br Vet J. 1984 Mar-Apr;140(2):202-6. PubMed PMID: 6586253.
9: Peeters JE, Geeroms R, Varewyck H, Bouquet Y, Lampo P, Halen P. Immunity and effect of clopidol/methyl benzoquate and robenidine before and after weaning on rabbit coccidiosis in the field. Res Vet Sci. 1983 Sep;35(2):211-6. PubMed PMID: 6356256.
10: Joyner LP, Catchpole J, Berrett S. Eimeria stiedai in rabbits: the demonstration of responses to chemotherapy. Res Vet Sci. 1983 Jan;34(1):64-7. PubMed PMID: 6132429.
11: Peeters JE, Geeroms R, Molderez J, Halen P. Activity of clopidol/methylbenzoquate, robenidine and salinomycin against hepatic coccidiosis in rabbits. Zentralbl Veterinarmed B. 1982 Apr;29(3):207-18. PubMed PMID: 6956190.
12: Mathis GF, McDougald LR. Drug responsiveness of field isolates of chicken Coccidia. Poult Sci. 1982 Jan;61(1):38-45. PubMed PMID: 7088782.
13: Kowalski LM, Reid WM. Effects of roxarsone on pigmentation and coccidiosis in broilers. Poult Sci. 1975 Sep;54(5):1544-9. PubMed PMID: 1187515.
14: Mitrovic M, Schildknecht EG. Lasalocid: resistance and cross-resistance studies in Eimeria tenella-infected chicks. Poult Sci. 1975 May;54(3):750-6. PubMed PMID: 1153374.
15: McLoughlin DK, Chute MB. Efficacy of nequinate against thirteen strains of Eimeria tenella and the development of a nequinate-resistant strain. Avian Dis. 1973 Oct-Dec;17(4):717-21. PubMed PMID: 4766564.
16: Cox PL. Collaborative study for the determination of nequinate in feeds. J Assoc Off Anal Chem. 1971 Jan;54(1):69-71. PubMed PMID: 5170699.
