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MedKoo Cat#: 561580 | Name: Rhodamine 6G
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Rhodamine 6G is a Rhodamine analog used in Pgp efflux assays and characterizing the kinetics of MRP1-mediated efflux. It is also utilized as a laser dye and potential mitochondrial probe.

Chemical Structure

Rhodamine 6G
Rhodamine 6G
CAS#989-38-8

Theoretical Analysis

MedKoo Cat#: 561580

Name: Rhodamine 6G

CAS#: 989-38-8

Chemical Formula: C28H31ClN2O3

Exact Mass:

Molecular Weight: 479.01

Elemental Analysis: C, 70.21; H, 6.52; Cl, 7.40; N, 5.85; O, 10.02

Price and Availability

Size Price Availability Quantity
25g USD 170.00
100g USD 305.00
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Synonym
Rhodamine 6G; Rhodamine6G; Rhodamine-6G; Basic Red 1
IUPAC/Chemical Name
Ethyl 2-[3-(ethylamino)-6-ethylimino-2,7-dimethylxanthen-9-yl]benzoate hydrochloride
InChi Key
XFKVYXCRNATCOO-UHFFFAOYSA-M
InChi Code
InChI=1S/C28H31N2O3.ClH/c1-6-29-23-15-25-21(13-17(23)4)27(19-11-9-10-12-20(19)28(31)32-8-3)22-14-18(5)24(30-7-2)16-26(22)33-25;/h9-16,29-30H,6-8H2,1-5H3;1H/q+1;/p-1
SMILES Code
CC1=CC2=C(C3=C(C=C(C(C)=C3)NCC)[O+]=C2C=C1NCC)C4=CC=CC=C4C(OCC)=O.[Cl-]
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO and water
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO and water
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
Rhodamine-6G is a fluorescent dye binding to mitochondria, thus reducing the intact mitochondria number and inhibiting mitochondrial metabolic activity.
In vitro activity:
Rhodamine-6G pre-treatment of LMTK cells permits the construction of transmitochondrial cybrid cell lines carrying a variety of mtDNAs, without the need for rho 0 cell lines. Reference: Somat Cell Mol Genet. 1996 Jan;22(1):81-5. https://pubmed.ncbi.nlm.nih.gov/8643997/
In vivo activity:
This study concludes that malignant, but not normal, cells are selectively destroyed by low doses of Rhodamine-6G. They suggest that selective anti-tumor properties of Rhodamine-6G are based on unique physiologic differences in energy metabolism between malignant and normal cells. It is possible that low concentrations of Rhodamine-6G might be useful for replacing, or backing up, more aggressive nonselective chemotherapeutic compounds. Reference: Neoplasma. 2013;60(3):262-73. https://pubmed.ncbi.nlm.nih.gov/23373995/
Solvent mg/mL mM
Solubility
DMSO 25.0 52.19
Water 10.0 20.88
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 479.01 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Magut PK, Das S, Fernand VE, Losso J, McDonough K, Naylor BM, Aggarwal S, Warner IM. Tunable cytotoxicity of rhodamine 6G via anion variations. J Am Chem Soc. 2013 Oct 23;135(42):15873-9. doi: 10.1021/ja407164w. Epub 2013 Oct 8. PMID: 24059469; PMCID: PMC4197813. 2. Trounce I, Wallace DC. Production of transmitochondrial mouse cell lines by cybrid rescue of rhodamine-6G pre-treated L-cells. Somat Cell Mol Genet. 1996 Jan;22(1):81-5. doi: 10.1007/BF02374379. PMID: 8643997. 3. Kutushov M, Gorelik O. Low concentrations of Rhodamine-6G selectively destroy tumor cells and improve survival of melanoma transplanted mice. Neoplasma. 2013;60(3):262-73. doi: 10.4149/neo_2013_035. PMID: 23373995. 4. Li C, Feng X, Yang S, Xu H, Yin X, Yu Y. Capture, Detection, and Simultaneous Identification of Rare Circulating Tumor Cells Based on a Rhodamine 6G-Loaded Metal-Organic Framework. ACS Appl Mater Interfaces. 2021 Oct 28. doi: 10.1021/acsami.1c15838. Epub ahead of print. PMID: 34709779.
In vitro protocol:
1. Magut PK, Das S, Fernand VE, Losso J, McDonough K, Naylor BM, Aggarwal S, Warner IM. Tunable cytotoxicity of rhodamine 6G via anion variations. J Am Chem Soc. 2013 Oct 23;135(42):15873-9. doi: 10.1021/ja407164w. Epub 2013 Oct 8. PMID: 24059469; PMCID: PMC4197813. 2. Trounce I, Wallace DC. Production of transmitochondrial mouse cell lines by cybrid rescue of rhodamine-6G pre-treated L-cells. Somat Cell Mol Genet. 1996 Jan;22(1):81-5. doi: 10.1007/BF02374379. PMID: 8643997.
In vivo protocol:
1. Kutushov M, Gorelik O. Low concentrations of Rhodamine-6G selectively destroy tumor cells and improve survival of melanoma transplanted mice. Neoplasma. 2013;60(3):262-73. doi: 10.4149/neo_2013_035. PMID: 23373995. 2. Li C, Feng X, Yang S, Xu H, Yin X, Yu Y. Capture, Detection, and Simultaneous Identification of Rare Circulating Tumor Cells Based on a Rhodamine 6G-Loaded Metal-Organic Framework. ACS Appl Mater Interfaces. 2021 Oct 28. doi: 10.1021/acsami.1c15838. Epub ahead of print. PMID: 34709779.
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