Synonym
CID 49766530; CID-49766530; CID49766530; ML-210; ML 210; ML210; DPI 10; DPI compound 10; SML 0521; SML-0521; SML0521;
IUPAC/Chemical Name
[4-[Bis(4-chlorophenyl)methyl]piperazin-1-yl]-(5-methyl-4-nitro-1,2-oxazol-3-yl)methanone
InChi Key
VIBHJPDPEVVDTB-UHFFFAOYSA-N
InChi Code
InChI=1S/C22H20Cl2N4O4/c1-14-20(28(30)31)19(25-32-14)22(29)27-12-10-26(11-13-27)21(15-2-6-17(23)7-3-15)16-4-8-18(24)9-5-16/h2-9,21H,10-13H2,1H3
SMILES Code
O=C(N1CCN(C(C2=CC=C(Cl)C=C2)C3=CC=C(Cl)C=C3)CC1)C4=NOC(C)=C4[N+]([O-])=O
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
Biological target:
ML-210 is a glutathione peroxidase 4 (GPX4) inhibitor with an EC50 of 30 nM.
In vitro activity:
Herein, by mixing the metal-phenolic network formed by tannic acid (TA), bleomycin (BLM), and Fe3+ with glutathione peroxidase 4 (GPX4) inhibitor (ML210) loaded hollow mesoporous Prussian blue (HMPB) nanocubes, the HMPB/ML210@TA-BLM-Fe3+ (HMTBF) nanocomplex is prepared to favor the ferroptosis/apoptosis synergism in TNBC. During the intracellular degradation, Fe3+ /Fe2+ conversion mediated by TA can initiate the Fenton reaction to drastically upregulate the reactive oxygen species level in cells, subsequently induce the accumulation of lipid peroxidation, and thereby cause ferroptotic cell death; meanwhile, the released ML210 efficiently represses the activity of GPX4 to activate ferroptosis pathway.
Reference: Small. 2021 Nov;17(47):e2103919. https://pubmed.ncbi.nlm.nih.gov/34623753/
|
Solvent |
mg/mL |
mM |
comments |
| Solubility |
| DMSO |
16.6 |
34.86 |
|
| DMF |
10.0 |
21.04 |
|
| DMF:PBS (pH 7.2) (1:1) |
0.5 |
1.05 |
|
| Ethanol |
4.0 |
8.42 |
|
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.
Preparing Stock Solutions
The following data is based on the
product
molecular weight
475.33
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
| 1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
| 5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
| 10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
| 50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
Formulation protocol:
1. Tong ZB, Kim H, El Touny L, Simeonov A, Gerhold D. LUHMES Dopaminergic Neurons Are Uniquely Susceptible to Ferroptosis. Neurotox Res. 2022 Oct;40(5):1526-1536. doi: 10.1007/s12640-022-00538-y. Epub 2022 Aug 4. Erratum in: Neurotox Res. 2022 Aug 27;: PMID: 35922689; PMCID: PMC9576307.
2. Zhou L, Chen J, Li R, Wei L, Xiong H, Wang C, Chai K, Chen M, Zhu Z, Yao T, Lin Y, Dong C, Shi S. Metal-Polyphenol-Network Coated Prussian Blue Nanoparticles for Synergistic Ferroptosis and Apoptosis via Triggered GPX4 Inhibition and Concurrent In Situ Bleomycin Toxification. Small. 2021 Nov;17(47):e2103919. doi: 10.1002/smll.202103919. Epub 2021 Oct 8. PMID: 34623753.
In vitro protocol:
1. Tong ZB, Kim H, El Touny L, Simeonov A, Gerhold D. LUHMES Dopaminergic Neurons Are Uniquely Susceptible to Ferroptosis. Neurotox Res. 2022 Oct;40(5):1526-1536. doi: 10.1007/s12640-022-00538-y. Epub 2022 Aug 4. Erratum in: Neurotox Res. 2022 Aug 27;: PMID: 35922689; PMCID: PMC9576307.
2. Zhou L, Chen J, Li R, Wei L, Xiong H, Wang C, Chai K, Chen M, Zhu Z, Yao T, Lin Y, Dong C, Shi S. Metal-Polyphenol-Network Coated Prussian Blue Nanoparticles for Synergistic Ferroptosis and Apoptosis via Triggered GPX4 Inhibition and Concurrent In Situ Bleomycin Toxification. Small. 2021 Nov;17(47):e2103919. doi: 10.1002/smll.202103919. Epub 2021 Oct 8. PMID: 34623753.
1: Bittker JA, Weiwer M, Lewis T, Shimada K, Yang WS, MacPherson L, Dandapani S,
Munoz B, Palmer M, Stockwell BR, Schreiber SL. Screen for RAS-Selective Lethal
Compounds and VDAC Ligands - Probe 2. 2011 Feb 10 [updated 2011 Dec 12]. Probe
Reports from the NIH Molecular Libraries Program [Internet]. Bethesda (MD):
National Center for Biotechnology Information (US); 2010-. Available from
http://www.ncbi.nlm.nih.gov/books/NBK98919/
PubMed PMID: 22834036.
2: Devarajan R, Ashraf M, Beck RO, Lemberger RJ, Taylor MC. Holmium: YAG
lasertripsy for ureteric calculi: an experience of 300 procedures. Br J Urol.
1998 Sep;82(3):342-7. PubMed PMID: 9772868.
3: Weïwer M, Bittker JA, Lewis TA, Shimada K, Yang WS, MacPherson L, Dandapani S,
Palmer M, Stockwell BR, Schreiber SL, Munoz B. Development of small-molecule
probes that selectively kill cells induced to express mutant RAS. Bioorg Med Chem
Lett. 2012 Feb 15;22(4):1822-6. doi: 10.1016/j.bmcl.2011.09.047. Epub 2011 Sep
28. PubMed PMID: 22297109; PubMed Central PMCID: PMC3528973.
