MedKoo Biosciences, Inc.
Tel:   +1-919-636-5577    Fax:   +1-919-980-4831    Email:   sales@medkoo.com
Search
Login Register
Search
MedKoo Cat#: 561330 | Name: Propidium Iodide
Featured

Description:

WARNING: This product is for research use only, not for human or veterinary use.

Propidium Iodide is a fluorescent intercalating agent that can be used to stain cells. Propidium Iodide is used as a DNA stain in flow cytometry to evaluate cell viability or DNA content in cell cycle analysis, and in microscopy to visualise the nucleus and other DNA-containing organelles. Propidium Iodide cannot cross the membrane of live cells, making it useful to differentiate necrotic, apoptotic and healthy cells.

Chemical Structure

Propidium Iodide
Propidium Iodide
CAS#25535-16-4

Theoretical Analysis

MedKoo Cat#: 561330

Name: Propidium Iodide

CAS#: 25535-16-4

Chemical Formula: C27H34I2N4

Exact Mass: 0.0000

Molecular Weight: 668.41

Elemental Analysis: C, 48.52; H, 5.13; I, 37.97; N, 8.38

Price and Availability

Size Price Availability Quantity
25mg USD 300.00
50mg USD 440.00
100mg USD 700.00
Bulk Inquiry
Buy Now
Add to Cart
Related CAS #
No Data
Synonym
Propidium Iodide; Propidium Diodide;
IUPAC/Chemical Name
3-(3,8-Diamino-6-phenylphenanthridin-5-ium-5-yl)propyl-diethyl-methylazanium diiodide
InChi Key
XJMOSONTPMZWPB-UHFFFAOYSA-M
InChi Code
InChI=1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
SMILES Code
C[N+](CC)(CCC[N+]1=C(C2=CC=CC=C2)C3=CC(N)=CC=C3C4=C1C=C(N)C=C4)CC.[I-].[I-]
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info

Preparing Stock Solutions

The following data is based on the product molecular weight 668.41 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1: Ahmad SS. An introduction to DARC technology. Saudi J Ophthalmol. 2017 Jan-Mar;31(1):38-41. doi: 10.1016/j.sjopt.2016.08.001. Epub 2016 Aug 22. Review. PubMed PMID: 28337061; PubMed Central PMCID: PMC5352956. 2: Zeng D, Chen Z, Jiang Y, Xue F, Li B. Advances and Challenges in Viability Detection of Foodborne Pathogens. Front Microbiol. 2016 Nov 22;7:1833. eCollection 2016. Review. PubMed PMID: 27920757; PubMed Central PMCID: PMC5118415. 3: Grant RL, Jenkins AF. Use of In Vivo and In Vitro Data to Derive a Chronic Reference Value for Crotonaldehyde Based on Relative Potency to Acrolein. J Toxicol Environ Health B Crit Rev. 2015;18(7-8):327-43. doi: 10.1080/10937404.2015.1081574. Epub 2015 Nov 18. Review. PubMed PMID: 26580244; PubMed Central PMCID: PMC4706029. 4: Marques LS, Bos-Mikich A, Godoy LC, Silva LA, Maschio D, Zhang T, Streit DP Jr. Viability of zebrafish (Danio rerio) ovarian follicles after vitrification in a metal container. Cryobiology. 2015 Dec;71(3):367-73. doi: 10.1016/j.cryobiol.2015.09.004. Epub 2015 Sep 25. Review. PubMed PMID: 26408854. 5: Gibellini L, De Biasi S, Nasi M, Carnevale G, Pisciotta A, Bianchini E, Bartolomeo R, Polo M, De Pol A, Pinti M, Cossarizza A. Different origin of adipogenic stem cells influences the response to antiretroviral drugs. Exp Cell Res. 2015 Oct 1;337(2):160-9. doi: 10.1016/j.yexcr.2015.07.031. Epub 2015 Jul 31. Review. PubMed PMID: 26238601. 6: Kepp O, Senovilla L, Vitale I, Vacchelli E, Adjemian S, Agostinis P, Apetoh L, Aranda F, Barnaba V, Bloy N, Bracci L, Breckpot K, Brough D, Buqué A, Castro MG, Cirone M, Colombo MI, Cremer I, Demaria S, Dini L, Eliopoulos AG, Faggioni A, Formenti SC, Fučíková J, Gabriele L, Gaipl US, Galon J, Garg A, Ghiringhelli F, Giese NA, Guo ZS, Hemminki A, Herrmann M, Hodge JW, Holdenrieder S, Honeychurch J, Hu HM, Huang X, Illidge TM, Kono K, Korbelik M, Krysko DV, Loi S, Lowenstein PR, Lugli E, Ma Y, Madeo F, Manfredi AA, Martins I, Mavilio D, Menger L, Merendino N, Michaud M, Mignot G, Mossman KL, Multhoff G, Oehler R, Palombo F, Panaretakis T, Pol J, Proietti E, Ricci JE, Riganti C, Rovere-Querini P, Rubartelli A, Sistigu A, Smyth MJ, Sonnemann J, Spisek R, Stagg J, Sukkurwala AQ, Tartour E, Thorburn A, Thorne SH, Vandenabeele P, Velotti F, Workenhe ST, Yang H, Zong WX, Zitvogel L, Kroemer G, Galluzzi L. Consensus guidelines for the detection of immunogenic cell death. Oncoimmunology. 2014 Dec 13;3(9):e955691. eCollection 2014 Oct. Review. PubMed PMID: 25941621; PubMed Central PMCID: PMC4292729. 7: Tumir LM, Radić Stojković M, Piantanida I. Come-back of phenanthridine and phenanthridinium derivatives in the 21st century. Beilstein J Org Chem. 2014 Dec 10;10:2930-54. doi: 10.3762/bjoc.10.312. eCollection 2014. Review. PubMed PMID: 25550761; PubMed Central PMCID: PMC4273281. 8: Izdebska M, Klimaszewska-Wiśniewska A, Lewandowski D, Marcin Nowak J, Gagat M, Grzanka A. Arsenic trioxide preferentially induces nonapoptotic cell deaths as well as actin cytoskeleton rearrangement in the CHO AA8 cell line. Postepy Hig Med Dosw (Online). 2014 Dec 21;68:1492-500. Review. PubMed PMID: 25531713. 9: Trzcińska M, Bryła M, Gajda B, Gogol P. Fertility of boar semen cryopreserved in extender supplemented with butylated hydroxytoluene. Theriogenology. 2015 Feb;83(3):307-13. doi: 10.1016/j.theriogenology.2014.07.045. Epub 2014 Oct 30. Review. PubMed PMID: 25468554. 10: Sun M, Zhao W, Xie Q, Zhan Y, Wu B. Lentinan reduces tumor progression by enhancing gemcitabine chemotherapy in urothelial bladder cancer. Surg Oncol. 2015 Mar;24(1):28-34. doi: 10.1016/j.suronc.2014.11.002. Epub 2014 Nov 20. Review. PubMed PMID: 25434982. 11: Atale N, Gupta S, Yadav UC, Rani V. Cell-death assessment by fluorescent and nonfluorescent cytosolic and nuclear staining techniques. J Microsc. 2014 Jul;255(1):7-19. doi: 10.1111/jmi.12133. Epub 2014 May 15. Review. PubMed PMID: 24831993. 12: Davis C. Enumeration of probiotic strains: Review of culture-dependent and alternative techniques to quantify viable bacteria. J Microbiol Methods. 2014 Aug;103:9-17. doi: 10.1016/j.mimet.2014.04.012. Epub 2014 May 9. Review. PubMed PMID: 24814752. 13: Elizaquível P, Aznar R, Sánchez G. Recent developments in the use of viability dyes and quantitative PCR in the food microbiology field. J Appl Microbiol. 2014 Jan;116(1):1-13. doi: 10.1111/jam.12365. Epub 2013 Nov 1. Review. PubMed PMID: 24119073. 14: Gensberger ET, Kostić T. Novel tools for environmental virology. Curr Opin Virol. 2013 Feb;3(1):61-8. doi: 10.1016/j.coviro.2012.11.005. Epub 2012 Dec 14. Review. PubMed PMID: 23246441. 15: Fittipaldi M, Nocker A, Codony F. Progress in understanding preferential detection of live cells using viability dyes in combination with DNA amplification. J Microbiol Methods. 2012 Nov;91(2):276-89. doi: 10.1016/j.mimet.2012.08.007. Epub 2012 Aug 23. Review. PubMed PMID: 22940102. 16: Pohanka M. Cholinesterases, a target of pharmacology and toxicology. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2011 Sep;155(3):219-29. doi: 10.5507/bp.2011.036. Review. PubMed PMID: 22286807. 17: van Frankenhuyzen JK, Trevors JT, Lee H, Flemming CA, Habash MB. Molecular pathogen detection in biosolids with a focus on quantitative PCR using propidium monoazide for viable cell enumeration. J Microbiol Methods. 2011 Dec;87(3):263-72. doi: 10.1016/j.mimet.2011.09.007. Epub 2011 Sep 22. Review. PubMed PMID: 21963489. 18: Giordano G, Hong S, Faustman EM, Costa LG. Measurements of cell death in neuronal and glial cells. Methods Mol Biol. 2011;758:171-8. doi: 10.1007/978-1-61779-170-3_11. Review. PubMed PMID: 21815065. 19: Shukla KK, Mahdi AA, Mishra V, Rajender S, Sankhwar SN, Patel D, Das M. Withania somnifera improves semen quality by combating oxidative stress and cell death and improving essential metal concentrations. Reprod Biomed Online. 2011 May;22(5):421-7. doi: 10.1016/j.rbmo.2011.01.010. Epub 2011 Mar 8. Review. PubMed PMID: 21388887. 20: Lee WK, Wolff NA, Thévenod F. Organic cation transporters: physiology, toxicology and special focus on ethidium as a novel substrate. Curr Drug Metab. 2009 Jul;10(6):617-31. Epub 2009 Jul 15. Review. PubMed PMID: 19702534.