MedKoo Biosciences, Inc.
Tel:   +1-919-636-5577    Fax:   +1-919-980-4831    Email:   sales@medkoo.com
Search
Login Register
Search
MedKoo Cat#: 540234 | Name: Isopropyl thiogalactoside
Featured

Description:

WARNING: This product is for research use only, not for human or veterinary use.

Isopropyl thiogalactoside is a galactose and allolactose mimic that induces activation of lac operon, stimulating protein expression.

Chemical Structure

Isopropyl thiogalactoside
Isopropyl thiogalactoside
CAS#367-93-1

Theoretical Analysis

MedKoo Cat#: 540234

Name: Isopropyl thiogalactoside

CAS#: 367-93-1

Chemical Formula: C9H18O5S

Exact Mass: 238.0875

Molecular Weight: 238.30

Elemental Analysis: C, 45.36; H, 7.61; O, 33.57; S, 13.45

Price and Availability

Size Price Availability Quantity
1g USD 250.00 2 Weeks
5g USD 450.00 2 Weeks
Bulk Inquiry
Buy Now
Add to Cart
Related CAS #
No Data
Synonym
Isopropy-beta-D-thiogalactopyranoside; Isopropyl-beta-D-thiogalactoside;
IUPAC/Chemical Name
(2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol
InChi Key
BPHPUYQFMNQIOC-NXRLNHOXSA-N
InChi Code
InChI=1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
SMILES Code
O[C@H]([C@H]([C@H]([C@@H](CO)O1)O)O)[C@@H]1SC(C)C
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info

Preparing Stock Solutions

The following data is based on the product molecular weight 238.30 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1: Mok MT, Henderson BR. A two-step fluorescence-activated cell sorting approach to isolate genetically modified mammalian cells with isopropyl-beta-D-thiogalactoside (IPTG)-inducible gene expression. Cytometry A. 2012 Feb;81(2):101-4. doi: 10.1002/cyto.a.22003. Epub 2012 Jan 3. PubMed PMID: 22271398. 2: Iarchuk OB, Troianovskaia IN, Matvienko NI. [Repression of beta-galactosidase synthesis by isopropyl thiogalactoside by the induction of antisense RNAs]. Dokl Akad Nauk SSSR. 1986;290(6):1499-502. Russian. PubMed PMID: 2433114. 3: Kumar H, Kasho V, Smirnova I, Finer-Moore JS, Kaback HR, Stroud RM. Structure of sugar-bound LacY. Proc Natl Acad Sci U S A. 2014 Feb 4;111(5):1784-8. doi: 10.1073/pnas.1324141111. Epub 2014 Jan 22. PubMed PMID: 24453216; PubMed Central PMCID: PMC3918835. 4: Tran H, Oliveira SM, Goncalves N, Ribeiro AS. Kinetics of the cellular intake of a gene expression inducer at high concentrations. Mol Biosyst. 2015 Sep;11(9):2579-87. doi: 10.1039/c5mb00244c. Epub 2015 Jul 30. PubMed PMID: 26223179. 5: Iniesta AA, García-Heras F, Abellón-Ruiz J, Gallego-García A, Elías-Arnanz M. Two systems for conditional gene expression in Myxococcus xanthus inducible by isopropyl-β-D-thiogalactopyranoside or vanillate. J Bacteriol. 2012 Nov;194(21):5875-85. doi: 10.1128/JB.01110-12. Epub 2012 Aug 24. PubMed PMID: 22923595; PubMed Central PMCID: PMC3486104. 6: Tripathi NK, Priya R, Shrivastava A. Production of recombinant Chikungunya virus envelope 2 protein in Escherichia coli. Appl Microbiol Biotechnol. 2014 Mar;98(6):2461-71. doi: 10.1007/s00253-013-5426-4. Epub 2013 Dec 13. PubMed PMID: 24337252. 7: Weitz M, Mückl A, Kapsner K, Berg R, Meyer A, Simmel FC. Communication and computation by bacteria compartmentalized within microemulsion droplets. J Am Chem Soc. 2014 Jan 8;136(1):72-5. doi: 10.1021/ja411132w. Epub 2013 Dec 24. PubMed PMID: 24358940. 8: Liu Z, Tian L, Chen Y, Mou H. Efficient extracellular production of κ-carrageenase in Escherichia coli: effects of wild-type signal sequence and process conditions on extracellular secretion. J Biotechnol. 2014 Sep 20;185:8-14. doi: 10.1016/j.jbiotec.2014.06.007. Epub 2014 Jun 11. PubMed PMID: 24929200. 9: Zhao W, Wang Z, Cai Q, Ou WB, Meng FG. [Detection of biohazardous materials in water upon the characteristics of fluorescent sensor Frex]. Huan Jing Ke Xue. 2014 Jun;35(6):2287-93. Chinese. PubMed PMID: 25158508. 10: Marbach A, Bettenbrock K. lac operon induction in Escherichia coli: Systematic comparison of IPTG and TMG induction and influence of the transacetylase LacA. J Biotechnol. 2012 Jan;157(1):82-8. doi: 10.1016/j.jbiotec.2011.10.009. Epub 2011 Nov 3. PubMed PMID: 22079752. 11: Saski CA, Feltus FA, Parida L, Haiminen N. BAC sequencing using pooled methods. Methods Mol Biol. 2015;1227:55-67. doi: 10.1007/978-1-4939-1652-8_3. PubMed PMID: 25239741. 12: Nguyen QA, Schumann W. Use of IPTG-inducible promoters for anchoring recombinant proteins on the Bacillus subtilis spore surface. Protein Expr Purif. 2014 Mar;95:67-76. doi: 10.1016/j.pep.2013.11.018. Epub 2013 Dec 8. PubMed PMID: 24326192. 13: Walsh MF, Hermann R, Lee JH, Chaturvedi L, Basson MD. Schlafen 3 Mediates the Differentiating Effects of Cdx2 in Rat IEC-Cdx2L1 Enterocytes. J Invest Surg. 2015;28(4):202-7. PubMed PMID: 26268420; PubMed Central PMCID: PMC4771065. 14: Panahi R, Vasheghani-Farahani E, Shojaosadati SA, Bambai B. [Auto-inducible expression system based on the SigB-dependent ohrB promoter in Bacillus subtilis]. Mol Biol (Mosk). 2014 Nov-Dec;48(6):970-6. Russian. PubMed PMID: 25845237. 15: Giannuzzi G, Lobefaro N, Paradies E, Vozza A, Punzi G, Marobbio CM. Overexpression in E. coli and purification of the L. pneumophila Lpp2981 protein. Mol Biotechnol. 2014 Feb;56(2):157-65. doi: 10.1007/s12033-013-9691-3. PubMed PMID: 23949658. 16: Kjaerulff S, Nielsen O. An IPTG-inducible derivative of the fission yeast nmt promoter. Yeast. 2015 Jun;32(6):469-78. doi: 10.1002/yea.3073. Epub 2015 Mar 31. Erratum in: Yeast. 2016 Jan;33(1):33. PubMed PMID: 25801050. 17: Sohoni SV, Nelapati D, Sathe S, Javadekar-Subhedar V, Gaikaiwari RP, Wangikar PP. Optimization of high cell density fermentation process for recombinant nitrilase production in E. coli. Bioresour Technol. 2015;188:202-8. doi: 10.1016/j.biortech.2015.02.038. Epub 2015 Feb 17. PubMed PMID: 25739996. 18: Deng X, Xiong G, Cong W, Liu Z, Qi C, Yang J. Monoclonal antibodies directed against VP7 protein of human group B rotavirus. Monoclon Antib Immunodiagn Immunother. 2014 Feb;33(1):38-41. doi: 10.1089/mab.2013.0060. PubMed PMID: 24555935. 19: Song D, Chen Y, Li X, Zhu M, Gu Q. Heterologous expression and purification of dermaseptin S4 fusion in Escherichia coli and recovery of biological activity. Prep Biochem Biotechnol. 2014;44(6):598-607. doi: 10.1080/10826068.2013.835735. PubMed PMID: 24499364. 20: Meyer S, Ramot R, Kishore Inampudi K, Luo B, Lin C, Amere S, Wilson CJ. Engineering alternate cooperative-communications in the lactose repressor protein scaffold. Protein Eng Des Sel. 2013 Jun;26(6):433-43. doi: 10.1093/protein/gzt013. Epub 2013 Apr 14. PubMed PMID: 23587523.