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MedKoo Cat#: 530668 | Name: Withaferin A
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Withaferin A is a steroidal lactone, derived from Acnistus arborescens,Withania somnifera (Indian Winter cherry or Ashwagandha in Sanskrit) and other members of Solanaceae family. It has been traditionally used in ayurvedic medicine. It is the first member of the withanolide class of ergostane type product to be discovered. This natural product has wide range of pharmacological activities including cardioprotective, anti-inflammatory, immuno-modulatory, anti-angiogenesis, anti-metastasis and anti-carcinogenic properties.

Chemical Structure

Withaferin A
Withaferin A
CAS#5119-48-2

Theoretical Analysis

MedKoo Cat#: 530668

Name: Withaferin A

CAS#: 5119-48-2

Chemical Formula: C28H38O6

Exact Mass: 470.2668

Molecular Weight: 470.61

Elemental Analysis: C, 71.46; H, 8.14; O, 20.40

Price and Availability

Size Price Availability Quantity
1mg USD 350.00 2 Weeks
5mg USD 750.00 2 Weeks
10mg USD 1,250.00 2 Weeks
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Related CAS #
No Data
Synonym
Withaferin A; NSC 101088; NSC-101088; NSC101088; NSC 273757; NSC-273757; NSC273757.
IUPAC/Chemical Name
(4S,4aR,5aR)-4-hydroxy-9-((2R,3R)-3-hydroxy-4-(4-methyl-5-oxo-2,5-dihydrofuran-3-yl)butan-2-yl)-9a,11b-dimethyl-5a,6,6a,6b,7,8,9,9a,10,11,11a,11b-dodecahydrocyclopenta[1,2]phenanthro[8a,9-b]oxiren-1(4H)-one
InChi Key
CQUCHTRQSNYVGJ-YBVJNLSVSA-N
InChi Code
InChI=1S/C28H38O6/c1-14-16(13-33-25(14)32)11-21(29)15(2)18-5-6-19-17-12-24-28(34-24)23(31)8-7-22(30)27(28,4)20(17)9-10-26(18,19)3/h7-8,15,17-21,23-24,29,31H,5-6,9-13H2,1-4H3/t15-,17?,18?,19?,20?,21-,23+,24-,26?,27?,28-/m1/s1
SMILES Code
CC12C(CCC2[C@@H](C)[C@H](O)CC(CO3)=C(C)C3=O)C4C[C@@H]5[C@](C6(C)C4CC1)(O5)[C@@H](O)C=CC6=O
Appearance
White to off-white solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info

Preparing Stock Solutions

The following data is based on the product molecular weight 470.61 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1: Nie HJ, Fu YJ, Long S, Wang JY, Zhao WS, Zhai LH, Yang YL, Tan MJ, Hu H, Chen XH. Chemoproteomics reveals proteome-wide covalent and non-covalent targets of withaferin A. Acta Pharmacol Sin. 2025 Feb 3. doi: 10.1038/s41401-024-01468-5. Epub ahead of print. PMID: 39900821. 2: Breen AK, Thomas S, Beckett D, Agsalud M, Gingras G, Williams J, Wasko BM. An mTOR inhibitor discovery system using drug-sensitized yeast. Geroscience. 2025 Jan 30. doi: 10.1007/s11357-025-01534-8. Epub ahead of print. PMID: 39885115. 3: Palomino GJQ, Ygnacio RHC, de Lima LF, Ferreira AS, Neto JEDC, Tetaping GM, Gomes FDR, Pessoa ODL, da Silva Raposo R, Rocha DD, Pessoa CDÓ, Figueiredo JR, de Sá NAR, Rodrigues APR. Investigations on the effects of in vitro exposure of mouse ovaries to withaferin A, a new candidate for chemotherapy. Reprod Toxicol. 2025 Jan 26;132:108844. doi: 10.1016/j.reprotox.2025.108844. Epub ahead of print. PMID: 39875005. 4: Zhang H, Kim H, Yuan T, Zhang Z, Kaul SC, Wadhwa R. Molecular Characterization of Cancer Preventive and Therapeutic Potential of Three Antistress Compounds, Triethylene Glycol, Withanone, and Withaferin A. Int J Mol Sci. 2025 Jan 9;26(2):493. doi: 10.3390/ijms26020493. PMID: 39859209; PMCID: PMC11764651. 5: Sharma KB, Subramani C, Ganesh K, Sharma A, Basu B, Balyan S, Sharma G, Ka S, Deb A, Srivastava M, Chugh S, Sehrawat S, Bharadwaj K, Rout A, Sahoo PK, Saurav S, Motiani RK, Singh R, Jain D, Asthana S, Wadhwa R, Vrati S. Withaferin A inhibits Chikungunya virus nsP2 protease and shows antiviral activity in the cell culture and mouse model of virus infection. PLoS Pathog. 2024 Dec 30;20(12):e1012816. doi: 10.1371/journal.ppat.1012816. PMID: 39775571; PMCID: PMC11723598. 6: Rahman MM, Tollefsbol TO. Combinatorial phenethyl isothiocyanate and withaferin A targets multiple epigenetics pathways to inhibit MCF-7 and MDA- MB-231 human breast cancer cells. Cancer Cell Int. 2024 Dec 20;24(1):422. doi: 10.1186/s12935-024-03619-4. PMID: 39707321; PMCID: PMC11662855. 7: Nisar MF, Wan C, Büsselberg D, Calina D, Sharifi-Rad J. Current mechanistic insights into Withaferin A: a promising potential adjuvant anticancer agent from Withania somnifera. Naunyn Schmiedebergs Arch Pharmacol. 2024 Nov 30. doi: 10.1007/s00210-024-03662-y. Epub ahead of print. PMID: 39614896. 8: Sherapura A, Kiran BK, Pavan Kumar GS, Siddesh BM, Thirusangu P, Suchetha Kumari N, Prabhakar BT. Withaferin-A induced vimentin S56 phosphorylation dissociates NEDD9 signaling loop to regress progressive metastatic melanoma into lung adenocarcinoma. Chem Biol Interact. 2025 Jan 25;406:111319. doi: 10.1016/j.cbi.2024.111319. Epub 2024 Nov 27. PMID: 39613173. 9: Speers AB, Lozano-Ortiz A, Soumyanath A. Quantifying Withanolides in Plasma: Pharmacokinetic Studies and Analytical Methods. Nutrients. 2024 Nov 8;16(22):3836. doi: 10.3390/nu16223836. PMID: 39599622; PMCID: PMC11597739. 10: Li D, Han H, Sun Y, Zhang H, Yoshitomi R, Kaul SC, Wadhwa R. Molecular Insights into the Inhibition of Lipid Accumulation in Hepatocytes by Unique Extracts of Ashwagandha. Int J Mol Sci. 2024 Nov 14;25(22):12256. doi: 10.3390/ijms252212256. PMID: 39596320; PMCID: PMC11594306. 11: Palomino GJQ, Celiz HY, Gomes FDR, Tetaping GM, Novaes MAS, Rocha KAD, Raposo RDS, Rocha RMP, Duarte ABG, Pessoa ODL, Figueiredo JR, de Sá NAR, Rodrigues APR. Withanolide derivatives: natural compounds with anticancer potential offer low toxicity to fertility and ovarian follicles in mice. Anim Reprod. 2024 Oct 21;21(4):e20240027. doi: 10.1590/1984-3143-AR2024-0027. PMID: 39494127; PMCID: PMC11529970. 12: Ahmad Mir S, Firdous S, Shahid Maqbool M, Hussain G, Yaqoob Bhat M, Malik FA, Khalid Yousuf S. Sonogashira coupling-based synthesis and in vitro cytotoxic evaluation of C-2 alkynyl derivatives of withaferin A. Steroids. 2024 Dec;212:109526. doi: 10.1016/j.steroids.2024.109526. Epub 2024 Oct 30. PMID: 39486668. 13: Hu H, Cao B, Huang D, Lin Y, Zhou B, Ying J, Huang L, Zhang L. Withaferin a modulation of microglia autophagy mitigates neuroinflammation and enhances cognitive function in POCD. Sci Rep. 2024 Oct 30;14(1):26112. doi: 10.1038/s41598-024-75284-6. PMID: 39478022; PMCID: PMC11525708. 14: Belka M, Gostyńska-Stawna A, Stawny M, Krajka-Kuźniak V. Activation of Nrf2 and FXR via Natural Compounds in Liver Inflammatory Disease. Int J Mol Sci. 2024 Oct 18;25(20):11213. doi: 10.3390/ijms252011213. PMID: 39456994; PMCID: PMC11508530. 15: Tan SY, Chen XX, Zhang R, Liu P, Wang JP, Wang T, Xiong ZE. SIRT3, a New Hope in Liver Diseases from Pathogenic Mechanisms to Therapeutic Strategies. Curr Pharm Biotechnol. 2024 Oct 24. doi: 10.2174/0113892010340592241011052133. Epub ahead of print. PMID: 39449346. 16: Jahagirdar S, Praveen Kumar H, Bhat SS, Poddar A, Chattaraj PK, Ahmad SF, Prasad SK. In silico evaluations of phytochemicals from Withania somnifera exhibiting anticancer activity against NAD[P]H-quinone oxidoreductase. Hum Exp Toxicol. 2024 Jan-Dec;43:9603271241291399. doi: 10.1177/09603271241291399. PMID: 39397382. 17: Kumar K, Bosch K, Vemuri V, Kratholm N, Rane M, Kakar SS. Withaferin A ameliorates ovarian cancer-induced renal damage through the regulation of expression of inflammatory cytokines. J Ovarian Res. 2024 Oct 11;17(1):199. doi: 10.1186/s13048-024-01519-9. PMID: 39394174; PMCID: PMC11468018. 18: Wang W, Chen H, Zhou Q. Identification and RT-qPCR Validation of Biomarkers Based on Butyrate Metabolism-Related Genes to Predict Recurrent Miscarriage. J Inflamm Res. 2024 Oct 1;17:6917-6934. doi: 10.2147/JIR.S470087. PMID: 39372587; PMCID: PMC11453136. 19: Khan A, Rehman AU, Siddiqui S, Khan J, Massey S, Singh P, Saluja D, Husain SA, Iqbal MA. Withaferin A decreases glycolytic reprogramming in breast cancer. Sci Rep. 2024 Oct 4;14(1):23147. doi: 10.1038/s41598-024-72221-5. PMID: 39366987; PMCID: PMC11452501. 20: Szydlak R. Synergistic Inhibition of Pancreatic Cancer Cell Growth and Migration by Gemcitabine and Withaferin A. Biomolecules. 2024 Sep 19;14(9):1178. doi: 10.3390/biom14091178. PMID: 39334944; PMCID: PMC11430445.