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MedKoo Cat#: 531021 | Name: Ethopabate
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Ethopabate is a folate metabolism inhibitor used in the prophylaxis and treatment of coccidiosis in chickens.

Chemical Structure

Ethopabate
Ethopabate
CAS#59-06-3

Theoretical Analysis

MedKoo Cat#: 531021

Name: Ethopabate

CAS#: 59-06-3

Chemical Formula: C12H15NO4

Exact Mass: 237.1001

Molecular Weight: 237.26

Elemental Analysis: C, 60.75; H, 6.37; N, 5.90; O, 26.97

Price and Availability

Size Price Availability Quantity
5g USD 150.00 Ready to ship
10g USD 250.00 Ready to ship
25g USD 450.00 Ready to ship
50g USD 750.00 Ready to ship
100g USD 1,250.00 Ready to ship
200g USD 1,850.00 Ready to ship
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Related CAS #
No Data
Synonym
Ethopabate, Amprol Plus, Ethyl pabate;
IUPAC/Chemical Name
methyl 4-acetamido-2-ethoxybenzoate
InChi Key
GOVWOKSKFSBNGD-UHFFFAOYSA-N
InChi Code
InChI=1S/C12H15NO4/c1-4-17-11-7-9(13-8(2)14)5-6-10(11)12(15)16-3/h5-7H,4H2,1-3H3,(H,13,14)
SMILES Code
O=C(OC)C1=CC=C(NC(C)=O)C=C1OCC
Appearance
White to light pink crystalline powder.
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Biological target:
Ethopabate is an antiprotozoal agent.
In vitro activity:
TBD
In vivo activity:
The purpose of this experiment was to study the relative limits of tolerance (in terms of growth) of broiler chicks for ethopabate (4-acetamido-2-ethoxylbenzoic acid methyl ester) when given in a ration containing amproHum, as these drugs are always combined in practice. In the first experiment, 450 day-old Nichols-Lohmann cockerels were divided into nine groups of 50 each and housed in floor pens. A second experiment was then carried out with groups of birds in which the ethopabate was varied from 100 to 300 p.p.m. In this study the addition of ethopabate in increments of 10 p.p.m. to feed containing 100 p.p.m. of amprolium had no significant influence on growth and feed consumption at the end of eight weeks. However, when 80 p.p.m. of ethopabate were added, the birds in Group 9 gained 9.1 % less weight than the controls during the first 14-day period, a significant difference (P = 0.05). Feed conversion was also 9.9% lower. The results of these experiments indicate that the tolerance of chickens for ethopabate is approximately 100 p.p.m. when it is administered in a growing ration containing 100 p.p.m. of amproHum. than in the control group. Reference: Poult Sci. 1969 May;48(3):1019-21. https://pubmed.ncbi.nlm.nih.gov/5389902/
Solvent mg/mL mM
Solubility
DMSO 50.0 210.75
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 237.26 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Kobow J, Akkilic M, Lüders H. Ethopabate tolerance in growing chickens. Poult Sci. 1969 May;48(3):1019-21. doi: 10.3382/ps.0481019. PMID: 5389902.
In vitro protocol:
TBD
In vivo protocol:
1. Kobow J, Akkilic M, Lüders H. Ethopabate tolerance in growing chickens. Poult Sci. 1969 May;48(3):1019-21. doi: 10.3382/ps.0481019. PMID: 5389902.
1: Nasr JJ, Shalan S. Spectrofluorimetric analysis of ethopabate in veterinary formulations with application to residue determination in chicken muscles and liver. Luminescence. 2014 Dec;29(8):1188-93. doi: 10.1002/bio.2683. Epub 2014 May 10. PubMed PMID: 24817251. 2: El-Kosasy AM, Hussein LA, Magdy N, Abbas MM. Sensitive spectrofluorimetric methods for determination of ethopabate and amprolium hydrochloride in chicken plasma and their residues in food samples. Spectrochim Acta A Mol Biomol Spectrosc. 2015;150:430-9. doi: 10.1016/j.saa.2015.05.082. Epub 2015 May 30. PubMed PMID: 26057097. 3: Hussein LA, Magdy N, Abbas MM. Five different spectrophotometric methods for determination of Amprolium hydrochloride and Ethopabate binary mixture. Spectrochim Acta A Mol Biomol Spectrosc. 2015 Mar 5;138:395-405. doi: 10.1016/j.saa.2014.11.073. Epub 2014 Nov 29. PubMed PMID: 25523045. 4: Tan HS, Ramachandran P, Cacini W. High performance liquid chromatographic assay of amprolium and ethopabate in chicken feed using solid-phase extraction. J Pharm Biomed Anal. 1996 Nov;15(2):259-65. PubMed PMID: 8933428. 5: Chapman HD. Chemotherapy of caecal coccidiosis: efficacy of toltrazuril, sulphaquinoxaline/pyrimethamine and amprolium/ethopabate, given in drinking water, against field isolates of Eimeria tenella. Res Vet Sci. 1989 May;46(3):419-20. PubMed PMID: 2740635. 6: Nagata T, Saeki M, Nakazawa H, Fujita M, Takabatake E. Sensitive determination of ethopabate residues in chicken tissues by liquid chromatography with fluorometric detection. J Assoc Off Anal Chem. 1985 Jan-Feb;68(1):27-8. PubMed PMID: 3980408. 7: Schronk LR, Colvin BM, Hanks AR, Bushway RJ. High pressure liquid chromatographic determination of ethopabate in feeds. J Assoc Off Anal Chem. 1977 Sep;60(5):1048-50. PubMed PMID: 893324. 8: Granja RH, Niño AM, Zucchetti RA, Niño RE, Salerno AG. Validation of a high-performance liquid chromatographic method with UV detection for the determination of ethopabate residues in poultry liver. J AOAC Int. 2008 Nov-Dec;91(6):1483-7. PubMed PMID: 19202812. 9: Smallidge RL Jr. Gas-liquid chromatographic determination of ethopabate in feed premixes. J Assoc Off Anal Chem. 1978 May;61(3):561-3. PubMed PMID: 649547. 10: Thorpe VA. Sample preparation of carbadox, furazolidone, nitrofurazone, and ethopabate in medicated feeds for high pressure liquid chromatography. J Assoc Off Anal Chem. 1980 Sep;63(5):981-4. PubMed PMID: 7410308. 11: Kan CA, van Leeuwen W, van Gend HW. [Amprolium residues in eggs following administration of amprolium/ethopabate in laying hens and breeding hens]. Tijdschr Diergeneeskd. 1989 Jan 15;114(2):76-82. Dutch. PubMed PMID: 2919391. 12: The determination of ethopabate in feeds. Analyst. 1967 Jul;92(96):468-71. PubMed PMID: 6068955. 13: Nagata T, Saeki M, Nakazawa H, Fujita M. Rapid quantitation of ethopabate in chicken muscles using high-performance liquid chromatography with fluorimetric detection: purification from extractant by continuous liquid-liquid partition. J Chromatogr. 1983 Dec 23;281:367-70. PubMed PMID: 6668337. 14: Buhs RP, Polin D, Beattie JO, Beck JL, Smith JL, Speth OC, Trenner NR. The metabolism of ethopabate in chickens. J Pharmacol Exp Ther. 1966 Nov;154(2):357-63. PubMed PMID: 5922996. 15: Cox GB, Sugden K. Determination of pyrimethamine, ethopabate and sulphaquinoxaline in poultry feeding stuffs by high-performance liquid chromatography using a weak cation-exchange column packing. Analyst. 1977 Jan;102(1210):29-34. PubMed PMID: 556893. 16: Rutczyńska-Skonieczna EM. [Detection of ethopabate residues in eggs]. Rocz Panstw Zakl Hig. 1980;31(2):181-5. Polish. PubMed PMID: 7414201. 17: Hamet-Foure N, Macar C, Robin B. Chemo-prophylaxis of turkey coccidiosis: Activity of clopidol with methylbenzoquate and amprolium with ethopabate against a mixed infection of Eimeria meleagrimitis and Eimeria adenoeides. Avian Pathol. 1979 Jan;8(1):107-13. PubMed PMID: 18770431. 18: Oe O, Arakawa A. Effect of feed additive antibiotics on chickens infected with Eimeria tenella. Poult Sci. 1975 Jul;54(4):1008-18. PubMed PMID: 1161694. 19: Kobow J, Akkilic M, Lüders H. Ethopabate tolerance in growing chickens. Poult Sci. 1969 May;48(3):1019-21. PubMed PMID: 5389902. 20: Arabkhazaeli F, Modrisanei M, Nabian S, Mansoori B, Madani A. Evaluating the resistance of eimeria spp. Field isolates to anticoccidial drugs using three different indices. Iran J Parasitol. 2013 Apr;8(2):234-41. PubMed PMID: 23914236; PubMed Central PMCID: PMC3724148.