SKL-2001 | agonist of Wnt/β-catenin signaling pathway | CAS#909089-13-0

MedKoo Cat#: 526260 | Name: SKL2001 free base

Description:

WARNING: This product is for research use only, not for human or veterinary use.

SKL-2001 is a novel agonist of Wnt/β-catenin signaling pathway. SKL2001 suppresses colon cancer spheroid growth through regulation of the E-cadherin/β-Catenin complex. SKL2001 inhibited proliferation of colon cancer cells cultured in 3D spheroid and induced them accumulation in the G0/G1 phase of the cell cycle with a reduced c-myc level.

Chemical Structure

SKL2001 free base

CAS#909089-13-0

Theoretical Analysis

MedKoo Cat#: 526260

Name: SKL2001 free base

CAS#: 909089-13-0

Chemical Formula: C14H14N4O3

Exact Mass: 286.1066

Molecular Weight: 286.29

Elemental Analysis: C, 58.74; H, 4.93; N, 19.57; O, 16.77

Price and Availability

Size	Price	Availability
50mg	USD 450.00	2 Weeks
100mg	USD 750.00	2 Weeks
200mg	USD 1,250.00	2 Weeks
500mg	USD 2,650.00	2 Weeks
1g	USD 3,650.00	2 Weeks
2g	USD 6,250.00	2 Weeks

Bulk Inquiry

Buy Now

Add to Cart

Related CAS #

No Data

Synonym

SKL-2001; SKL 2001; SKL2001; Wnt Agonist II;

IUPAC/Chemical Name

5-(Furan-2-yl)-N-(3-imidazol-1-ylpropyl)-1,2-oxazole-3-carboxamide

InChi Key

PQXINDBPUDNMPE-UHFFFAOYSA-N

InChi Code

InChI=1S/C14H14N4O3/c19-14(16-4-2-6-18-7-5-15-10-18)11-9-13(21-17-11)12-3-1-8-20-12/h1,3,5,7-10H,2,4,6H2,(H,16,19)

SMILES Code

O=C(C1=NOC(C2=CC=CO2)=C1)NCCCN3C=CN=C3

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

SKL2001 is an agonist of the Wnt/β-catenin pathway, with anti-cancer activity. SKL2001 stabilizes intracellular β-catenin via disruption of the Axin/β-catenin interaction.

In vitro activity:

SKL2001 has potential in colon cancer therapy. SKL2001 inhibited proliferation of colon cancer cells cultured in 3D spheroid and induced them accumulation in the G0/G1 phase of the cell cycle with a reduced c-myc level. SKL2001-treated cells resumed cell cycle progression and proliferated in the SKL2001-free medium. Notably, SKL2001 facilitated round-shape spheroid formation. Reference: Biochem Biophys Res Commun. 2017 Nov 25;493(3):1342-1348. https://pubmed.ncbi.nlm.nih.gov/28970068/

In vivo activity:

To be determined

	Solvent	mg/mL	mM
Solubility
	DMF	25.0	87.32
	DMSO	25.0	87.32
	Ethanol	25.0	87.32
	DMF	25.0	87.32
	DMSO	25.0	87.32
	Ethanol	25.0	87.32

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 286.29 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Liu Y, Ruan X, Li J, Wang B, Chen J, Wang X, Wang P, Tu X. The Osteocyte Stimulated by Wnt Agonist SKL2001 Is a Safe Osteogenic Niche Improving Bioactivities in a Polycaprolactone and Cell Integrated 3D Module. Cells. 2022 Feb 28;11(5):831. doi: 10.3390/cells11050831. PMID: 35269452; PMCID: PMC8909416. 2. Ohashi W, Yamamine N, Imura J, Hattori Y. SKL2001 suppresses colon cancer spheroid growth through regulation of the E-cadherin/β-Catenin complex. Biochem Biophys Res Commun. 2017 Nov 25;493(3):1342-1348. doi: 10.1016/j.bbrc.2017.09.161. Epub 2017 Sep 29. PMID: 28970068.

In vitro protocol:

In vivo protocol:

To be determined

1: Liang S, Zhu L, Yang F, Dong H. Transcription factor YY1-activated GNG5 facilitates glioblastoma cell growth, invasion, stemness and glycolysis through Wnt/β-catenin pathway. Sci Rep. 2024 Oct 24;14(1):25234. doi: 10.1038/s41598-024-76019-3. PMID: 39448763. 2: Zhang Z, Zhang D, Su K, Wu D, Hu Q, Jin T, Ye T, Zhang R. NTSR1 promotes epithelial-mesenchymal transition and metastasis in lung adenocarcinoma through the Wnt/β-catenin pathway. Mutat Res. 2024 Aug 10;829:111877. doi: 10.1016/j.mrfmmm.2024.111877. Epub ahead of print. PMID: 39180939. 3: Huang Z, Meng FY, Lu LZ, Guo QQ, Lv CJ, Tan NH, Deng Z, Chen JY, Zhang ZS, Zou B, Long HP, Zhou Q, Tian S, Mei S, Tian XF. Calculus bovis inhibits M2 tumor-associated macrophage polarization via Wnt/β-catenin pathway modulation to suppress liver cancer. World J Gastroenterol. 2024 Aug 7;30(29):3511-3533. doi: 10.3748/wjg.v30.i29.3511. PMID: 39156500; PMCID: PMC11326087. 4: Zhu F, Jiang J, Chen X, Fu L, Liu H, Zhang H. Amentoflavone regulates the miR-124-3p/CAPN2 axis to promote mitochondrial autophagy in HCC cells. Toxicol Res (Camb). 2024 Jul 23;13(4):tfae110. doi: 10.1093/toxres/tfae110. PMID: 39050595; PMCID: PMC11263925. 5: Hu G, Chen J, Chen M, Yang K, Wang Y, Ma Z, Bao H, Ding X. Silencing DOCK2 Attenuates Cardiac Fibrosis Following Myocardial Infarction in Mice Via Targeting PI3K/Akt and Wnt/β-Catenin Pathways. J Cardiovasc Transl Res. 2024 Jul 11. doi: 10.1007/s12265-024-10533-7. Epub ahead of print. PMID: 38990461. 6: Chen F, Cheng B, Xu X, Yan W, Meng Q, Liu J, Yao R, Dong F, Liu Y. High- intensity interval training stimulates remyelination via the Wnt/β-catenin pathway in cuprizone-induced demyelination mouse model. Neurol Res. 2024 Nov;46(11):996-1007. doi: 10.1080/01616412.2024.2376310. Epub 2024 Jul 9. PMID: 38979727. 7: Qiao Y, Chen Z, Li W, Li H, Zhou L. Clinical significance of TROAP in endometrial cancer and the antiproliferative and proapoptotic effects of TROAP knockdown in endometrial cancer cells: integrated utilization of bioinformatic analysis and in vitro test verification. Naunyn Schmiedebergs Arch Pharmacol. 2024 Jul 5. doi: 10.1007/s00210-024-03260-y. Epub ahead of print. PMID: 38967825. 8: Hao X, Wu X. SP1‑mediated ADAMTS5 transcription promotes IL‑1β‑induced chondrocyte injury via Wnt/β‑catenin pathway in osteoarthritis. Mol Med Rep. 2024 Aug;30(2):149. doi: 10.3892/mmr.2024.13273. Epub 2024 Jun 28. PMID: 38940327; PMCID: PMC11228694. 9: Wang Y, Lu Y, Xu C. Tensin 4 facilitates aerobic glycolysis, migration and invasion of colorectal cancer cells through the β‑catenin/c‑Myc signaling pathway. Oncol Lett. 2024 Jun 3;28(2):356. doi: 10.3892/ol.2024.14489. PMID: 38881712; PMCID: PMC11176887. 10: Yang F, Duan Y, Li Y, Zhu D, Wang Z, Luo Z, Zhang Y, Zhang G, He X, Kang X. S100A6 Regulates nucleus pulposus cell apoptosis via Wnt/β-catenin signaling pathway: an in vitro and in vivo study. Mol Med. 2024 Jun 14;30(1):87. doi: 10.1186/s10020-024-00853-4. PMID: 38877413; PMCID: PMC11179208. 11: Ke C, Zhou H, Xia T, Xie X, Jiang B. GTP binding protein 2 maintains the quiescence, self-renewal, and chemoresistance of mouse colorectal cancer stem cells via promoting Wnt signaling activation. Heliyon. 2024 Mar 1;10(5):e27159. doi: 10.1016/j.heliyon.2024.e27159. PMID: 38468952; PMCID: PMC10926081. 12: Li Y, Luan S, Ruan C, Li W, Zhang X, Ran Z, Bi W, Tong Y, Gao L, Zhao J, Li Y, He Z. TSHR signaling promotes hippocampal dependent memory formation through modulating Wnt5a/β-catenin mediated neurogenesis. Biochem Biophys Res Commun. 2024 Apr 16;704:149723. doi: 10.1016/j.bbrc.2024.149723. Epub 2024 Feb 28. PMID: 38430698. 13: Zhu S, Meng L, Wei P, Gu G, Duan K. Sinensetin suppresses breast cancer cell progression via Wnt/β-catenin pathway inhibition. Transl Cancer Res. 2024 Jan 31;13(1):348-362. doi: 10.21037/tcr-23-1317. Epub 2024 Jan 12. PMID: 38410229; PMCID: PMC10894327. 14: Li Z, Cai X. Baicalein targets STMN1 to inhibit the progression of nasopharyngeal carcinoma via regulating the Wnt/β-catenin pathway. Environ Toxicol. 2024 May;39(5):3003-3013. doi: 10.1002/tox.24173. Epub 2024 Feb 5. PMID: 38317500. 15: Fang F, Jin X, Meng J, He J, Wang J, Wang C, Xie S, Shi W. Jiedu Fuzheng decoction improves the proliferation, migration, invasion and EMT of non-small cell lung cancer via the Wnt/β-catenin pathway. Cell Div. 2023 Dec 16;18(1):22. doi: 10.1186/s13008-023-00105-7. PMID: 38104091; PMCID: PMC10725601. 16: Zhang H, Dong X, Ding X, Liu G, Yang F, Song Q, Sun H, Chen G, Li S, Li Y, Wang M, Guo T, Zhang Z, Li B, Yang L. Bufalin targeting CAMKK2 inhibits the occurrence and development of intrahepatic cholangiocarcinoma through Wnt/β-catenin signal pathway. J Transl Med. 2023 Dec 11;21(1):900. doi: 10.1186/s12967-023-04613-6. PMID: 38082327; PMCID: PMC10714474. 17: Zheng J, Yang H, Liu C, Zhang R, Yibulayimu N, Jin X. Ethanol Extract of Anacyclus pyrethrum Root Ameliorates Cough-Variant Asthma Through the TLR4/NF-κB Pathway and Wnt/β-Catenin Pathway. Mol Biotechnol. 2023 Nov 1. doi: 10.1007/s12033-023-00935-4. Epub ahead of print. PMID: 37910337. 18: Zhu Q, Hu Y, Jiang W, Ou ZL, Yao YB, Zai HY. Circ-CCT2 Activates Wnt/β-catenin Signaling to Facilitate Hepatoblastoma Development by Stabilizing PTBP1 mRNA. Cell Mol Gastroenterol Hepatol. 2024;17(2):175-197. doi: 10.1016/j.jcmgh.2023.10.004. Epub 2023 Oct 20. PMID: 37866478; PMCID: PMC10758885. 19: Wei S, Lin L, Jiang W, Chen J, Gong G, Sui D. Naked cuticle homolog 1 prevents mouse pulmonary arterial hypertension via inhibition of Wnt/β-catenin and oxidative stress. Aging (Albany NY). 2023 Oct 18;15(20):11114-11130. doi: 10.18632/aging.205105. Epub 2023 Oct 18. PMID: 37857014; PMCID: PMC10637826. 20: Cheng H, Li Q. Sevoflurane inhibits cholangiocarcinoma via Wnt/β-catenin signaling pathway. BMC Gastroenterol. 2023 Aug 11;23(1):279. doi: 10.1186/s12876-023-02911-3. PMID: 37568083; PMCID: PMC10422733.