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MedKoo Cat#: 525609 | Name: Ac-DEVD-pNA

Description:

WARNING: This product is for research use only, not for human or veterinary use.

Ac-DEVD-pNA is the preferred peptidic substrate for caspase-3.

Chemical Structure

Ac-DEVD-pNA
Ac-DEVD-pNA
CAS#189950-66-1

Theoretical Analysis

MedKoo Cat#: 525609

Name: Ac-DEVD-pNA

CAS#: 189950-66-1

Chemical Formula: C26H34N6O13

Exact Mass: 638.2184

Molecular Weight: 638.59

Elemental Analysis: C, 48.90; H, 5.37; N, 13.16; O, 32.57

Price and Availability

This product is currently not in stock but may be available through custom synthesis. To ensure cost efficiency, the minimum order quantity is 1 gram. The estimated lead time is 2 to 4 months, with pricing dependent on the complexity of the synthesis (typically high for intricate chemistries). Quotes for quantities below 1 gram will not be provided. To request a quote, please click the button below. Note: If this product becomes available in stock in the future, pricing will be listed accordingly.
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Related CAS #
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Synonym
Ac-DEVD-pNA
IUPAC/Chemical Name
N-Acetyl-Asp-Glu-Val-Asp p-nitroanilide
InChi Key
GGXRLUDNGFFUKI-ORGXJRBJSA-N
InChi Code
InChI=1S/C26H34N6O13/c1-12(2)22(26(43)30-18(11-21(38)39)24(41)28-14-4-6-15(7-5-14)32(44)45)31-23(40)16(8-9-19(34)35)29-25(42)17(10-20(36)37)27-13(3)33/h4-7,12,16-18,22H,8-11H2,1-3H3,(H,27,33)(H,28,41)(H,29,42)(H,30,43)(H,31,40)(H,34,35)(H,36,37)(H,38,39)/t16-,17-,18-,22-/m0/s1
SMILES Code
O=C(N[C@@H](CCC(O)=O)C(N[C@@H](C(C)C)C(N[C@@H](CC(O)=O)C(NC1=CC=C([N+]([O-])=O)C=C1)=O)=O)=O)[C@H](CC(O)=O)NC(C)=O
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info

Preparing Stock Solutions

The following data is based on the product molecular weight 638.59 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
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Characterization and cytotoxic activity of apoptosis-inducing pierisin-5 protein from white cabbage butterfly. Int J Biol Macromol. 2016 Jun;87:16-27. doi: 10.1016/j.ijbiomac.2016.01.072. Epub 2016 Jan 23. PubMed PMID: 26812112. 5: Wu MH, Jin XK, Yu AQ, Zhu YT, Li D, Li WW, Wang Q. Caspase-mediated apoptosis in crustaceans: cloning and functional characterization of EsCaspase-3-like protein from Eriocheir. Fish Shellfish Immunol. 2014 Dec;41(2):625-32. PubMed PMID: 25462457. 6: Yi HS, Pan CX, Pan C, Song J, Hu YF, Wang L, Pan MH, Lu C. BmICE-2 is a novel pro-apoptotic caspase involved in apoptosis in the silkworm, Bombyx mori. Biochem Biophys Res Commun. 2014 Feb 28;445(1):100-6. doi: 10.1016/j.bbrc.2014.01.139. Epub 2014 Jan 31. PubMed PMID: 24491540. 7: Wu J, Jing L, Yuan H, Peng SQ. T-2 toxin induces apoptosis in ovarian granulosa cells of rats through reactive oxygen species-mediated mitochondrial pathway. Toxicol Lett. 2011 May 10;202(3):168-77. doi: 10.1016/j.toxlet.2011.01.029. Epub 2011 Feb 4. PubMed PMID: 21296132. 8: Li M, Ding Y, Mu Y, Ao J, Chen X. Molecular cloning and characterization of caspase-3 in large yellow croaker (Pseudosciaena crocea). Fish Shellfish Immunol. 2011 Mar;30(3):910-6. doi: 10.1016/j.fsi.2011.01.018. Epub 2011 Feb 1. PubMed PMID: 21288491. 9: Peterson QP, Goode DR, West DC, Botham RC, Hergenrother PJ. Preparation of the caspase-3/7 substrate Ac-DEVD-pNA by solution-phase peptide synthesis. Nat Protoc. 2010 Feb;5(2):294-302. doi: 10.1038/nprot.2009.223. Epub 2010 Jan 28. PubMed PMID: 20134429; PubMed Central PMCID: PMC2921128. 10: Almeida S, Cunha-Oliveira T, Laço M, Oliveira CR, Rego AC. Dysregulation of CREB activation and histone acetylation in 3-nitropropionic acid-treated cortical neurons: prevention by BDNF and NGF. Neurotox Res. 2010 May;17(4):399-405. doi: 10.1007/s12640-009-9116-z. Epub 2009 Sep 25. PubMed PMID: 19779956. 11: Calafat M, Larocca L, Roca V, Hauk V, Pregi N, Nesse A, Pérez Leirós C. Vasoactive intestinal peptide inhibits TNF-alpha-induced apoptotic events in acinar cells from nonobese diabetic mice submandibular glands. Arthritis Res Ther. 2009;11(2):R53. doi: 10.1186/ar2671. Epub 2009 Apr 8. PubMed PMID: 19356238; PubMed Central PMCID: PMC2688204. 12: Dineva JD, Vangelov IM, Nikolov GG, Konakchieva RTs, Ivanova MD. Nitric oxide stimulates the production of atrial natriuretic peptide and progesterone by human granulosa luteinized cells with an antiapoptotic effect. Endocr Regul. 2008 Jun;42(2-3):45-51. PubMed PMID: 18624606. 13: Böing AN, Hau CM, Sturk A, Nieuwland R. Platelet microparticles contain active caspase 3. Platelets. 2008 Mar;19(2):96-103. doi: 10.1080/09537100701777295. PubMed PMID: 18297548. 14: Mao J, Liu S, Wen D, Tan X, Fu C. Basic fibroblast growth factor suppresses retinal neuronal apoptosis in form-deprivation myopia in chicks. Curr Eye Res. 2006 Nov;31(11):983-7. PubMed PMID: 17114124. 15: Jin Y, Yan EZ, Fan Y, Zong ZH, Qi ZM, Li Z. Sodium ferulate prevents amyloid-beta-induced neurotoxicity through suppression of p38 MAPK and upregulation of ERK-1/2 and Akt/protein kinase B in rat hippocampus. Acta Pharmacol Sin. 2005 Aug;26(8):943-51. PubMed PMID: 16038626. 16: Liu SZ, Mao JF, Wen D, Tan XP, Fu CY. [The treatment of retinal apoptosis by Caspase 3 inhibitor Ac-DEVD-CHO in experimental myopia]. Zhonghua Yan Ke Za Zhi. 2005 May;41(5):428-33. Chinese. PubMed PMID: 15938808. 17: Li T, Shi N, Zhong YF, Dong J, Chen L, Wang B, Chen D, Wei YH, Dai ZH. [Effects of deltamethrin on the apoptosis and the expression of caspase-3 in rat neural cells]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2004 Oct;22(5):371-4. Chinese. PubMed PMID: 15555316. 18: Chae IH, Park KW, Kim HS, Oh BH. Nitric oxide-induced apoptosis is mediated by Bax/Bcl-2 gene expression, transition of cytochrome c, and activation of caspase-3 in rat vascular smooth muscle cells. Clin Chim Acta. 2004 Mar;341(1-2):83-91. PubMed PMID: 14967162. 19: Dontenwill M, Pascal G, Piletz JE, Chen M, Baldwin J, Rondé P, Dupuy L, Urosevic D, Greney H, Takeda K, Bousquet P. IRAS, the human homologue of Nischarin, prolongs survival of transfected PC12 cells. Cell Death Differ. 2003 Aug;10(8):933-5. PubMed PMID: 12868002. 20: Ha KS, Kim KM, Kwon YG, Bai SK, Nam WD, Yoo YM, Kim PK, Chung HT, Billiar TR, Kim YM. Nitric oxide prevents 6-hydroxydopamine-induced apoptosis in PC12 cells through cGMP-dependent PI3 kinase/Akt activation. FASEB J. 2003 Jun;17(9):1036-47. PubMed PMID: 12773486.

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