MedKoo Biosciences, Inc.
Tel:   +1-919-636-5577    Fax:   +1-919-980-4831    Email:   sales@medkoo.com
Search
Login Register
Search
MedKoo Cat#: 525344 | Name: RCM-1
Featured New

Description:

WARNING: This product is for research use only, not for human or veterinary use.

RCM-1 is a nontoxic FOXM1 inhibitor, inhibiting goblet cell metaplasia and excessive mucus production.

Chemical Structure

RCM-1
RCM-1
CAS#339163-65-4

Theoretical Analysis

MedKoo Cat#: 525344

Name: RCM-1

CAS#: 339163-65-4

Chemical Formula: C20H12N2OS4

Exact Mass: 423.9832

Molecular Weight: 424.57

Elemental Analysis: C, 56.58; H, 2.85; N, 6.60; O, 3.77; S, 30.20

Price and Availability

Size Price Availability Quantity
5mg USD 325.00 2 Weeks
10mg USD 565.00 2 Weeks
Bulk Inquiry
Buy Now
Add to Cart
Related CAS #
No Data
Synonym
RCM-1; RCM1; RCM 1
IUPAC/Chemical Name
2-{[2-Oxo-2-(thiophen-2-yl)ethyl]sulfanyl}-4,6-di(thiophen-2-yl)pyridine-3- carbonitrile
InChi Key
DCCYTKKDAZEVLY-UHFFFAOYSA-N
InChi Code
InChI=1S/C20H12N2OS4/c21-11-14-13(17-4-1-7-24-17)10-15(18-5-2-8-25-18)22-20(14)27-12-16(23)19-6-3-9-26-19/h1-10H,12H2
SMILES Code
N#CC1=C(C2=CC=CS2)C=C(C3=CC=CS3)N=C1SCC(C4=CC=CS4)=O
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Certificate of Analysis
View CoA: current batch, Lot#AOB80925
Safety Data Sheet (SDS)
View Safety Data Sheet (SDS)
Instruction
View handling instruction
Biological target:
RCM 1 is a forkhead box M1 (FOXM1) transcription factor inhibitor (IC50 = 0.72 μM). Inhibits expression of FOXM1 target genes. Induces FOXM1 protein degradation in vitro. Reduces airway resistance, increases lung compliance and decreases proinflammatory cytokine production in allergen-exposed mice.
In vitro activity:
RCM-1 decreased protein levels and nuclear localization of β-catenin, and inhibited protein-protein interaction between β-catenin and FOXM1 in cultured tumor cells. RCM-1 could be a promising candidate for anticancer therapy. Reference: Mol Cancer Ther. 2019 Jul;18(7):1217-1229. https://pubmed.ncbi.nlm.nih.gov/31040162/
In vivo activity:
The results of this study suggest that RCM-1 is an inhibitor of goblet cell metaplasia and IL-13 signaling. These results provide a new therapeutic candidate to treat patients with asthma and other chronic airway diseases. Reference: Sci Signal. 2017 Apr 18;10(475):eaai8583. https://pubmed.ncbi.nlm.nih.gov/28420758/
Solvent mg/mL mM
Solubility
DMSO 8.5 20.00
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 424.57 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Kamimura M, Sasaki A, Otani Y, Nakamura Y, Nakamura T, Kuramochi K, Imai T, Kubo N, Okamoto S. Methylthioacetic acid, a derivative of aroma compounds from Cucumis melo var. conomon dose-dependently triggers differentiation and apoptosis of RCM-1 human colorectal cancer cells. J Toxicol Sci. 2023;48(1):25-35. doi: 10.2131/jts.48.25. PMID: 36599425. 2. Shukla S, Milewski D, Pradhan A, Rama N, Rice K, Le T, Flick MJ, Vaz S, Zhao X, Setchell KD, Logarinho E, Kalinichenko VV, Kalin TV. The FOXM1 Inhibitor RCM-1 Decreases Carcinogenesis and Nuclear β-Catenin. Mol Cancer Ther. 2019 Jul;18(7):1217-1229. doi: 10.1158/1535-7163.MCT-18-0709. Epub 2019 Apr 30. PMID: 31040162; PMCID: PMC7341442. 3. Sun L, Ren X, Wang IC, Pradhan A, Zhang Y, Flood HM, Han B, Whitsett JA, Kalin TV, Kalinichenko VV. The FOXM1 inhibitor RCM-1 suppresses goblet cell metaplasia and prevents IL-13 and STAT6 signaling in allergen-exposed mice. Sci Signal. 2017 Apr 18;10(475):eaai8583. doi: 10.1126/scisignal.aai8583. PMID: 28420758.
In vitro protocol:
1. Kamimura M, Sasaki A, Otani Y, Nakamura Y, Nakamura T, Kuramochi K, Imai T, Kubo N, Okamoto S. Methylthioacetic acid, a derivative of aroma compounds from Cucumis melo var. conomon dose-dependently triggers differentiation and apoptosis of RCM-1 human colorectal cancer cells. J Toxicol Sci. 2023;48(1):25-35. doi: 10.2131/jts.48.25. PMID: 36599425. 2. Shukla S, Milewski D, Pradhan A, Rama N, Rice K, Le T, Flick MJ, Vaz S, Zhao X, Setchell KD, Logarinho E, Kalinichenko VV, Kalin TV. The FOXM1 Inhibitor RCM-1 Decreases Carcinogenesis and Nuclear β-Catenin. Mol Cancer Ther. 2019 Jul;18(7):1217-1229. doi: 10.1158/1535-7163.MCT-18-0709. Epub 2019 Apr 30. PMID: 31040162; PMCID: PMC7341442.
In vivo protocol:
1. Sun L, Ren X, Wang IC, Pradhan A, Zhang Y, Flood HM, Han B, Whitsett JA, Kalin TV, Kalinichenko VV. The FOXM1 inhibitor RCM-1 suppresses goblet cell metaplasia and prevents IL-13 and STAT6 signaling in allergen-exposed mice. Sci Signal. 2017 Apr 18;10(475):eaai8583. doi: 10.1126/scisignal.aai8583. PMID: 28420758.
1: Shukla S, Milewski D, Pradhan A, Rama N, Rice K, Le T, Flick MJ, Vaz S, Zhao X, Setchell KD, Logarinho E, Kalinichenko VV, Kalin TV. The FOXM1 Inhibitor RCM-1 Decreases Carcinogenesis and Nuclear β-Catenin. Mol Cancer Ther. 2019 Jul;18(7):1217-1229. doi: 10.1158/1535-7163.MCT-18-0709. Epub 2019 Apr 30. PMID: 31040162; PMCID: PMC7341442. 2: Aldabbous MS, Roca MG, Stout A, Huang IC, Read ND, Free SJ. The ham-5, rcm-1 and rco-1 genes regulate hyphal fusion in Neurospora crassa. Microbiology (Reading). 2010 Sep;156(Pt 9):2621-2629. doi: 10.1099/mic.0.040147-0. Epub 2010 Jun 3. PMID: 20522492; PMCID: PMC3068686. 3: Cupertino FB, Virgilio S, Freitas FZ, Candido Tde S, Bertolini MC. Regulation of glycogen metabolism by the CRE-1, RCO-1 and RCM-1 proteins in Neurospora crassa. The role of CRE-1 as the central transcriptional regulator. Fungal Genet Biol. 2015 Apr;77:82-94. doi: 10.1016/j.fgb.2015.03.011. Epub 2015 Apr 16. PMID: 25889113. 4: Ruger-Herreros C, Gil-Sánchez Mdel M, Sancar G, Brunner M, Corrochano LM. Alteration of light-dependent gene regulation by the absence of the RCO-1/RCM-1 repressor complex in the fungus Neurospora crassa. PLoS One. 2014 Apr 18;9(4):e95069. doi: 10.1371/journal.pone.0095069. PMID: 24747913; PMCID: PMC3991626. 5: Olmedo M, Navarro-Sampedro L, Ruger-Herreros C, Kim SR, Jeong BK, Lee BU, Corrochano LM. A role in the regulation of transcription by light for RCO-1 and RCM-1, the Neurospora homologs of the yeast Tup1-Ssn6 repressor. Fungal Genet Biol. 2010 Nov;47(11):939-52. doi: 10.1016/j.fgb.2010.08.001. Epub 2010 Aug 10. PMID: 20709620. 6: Kamimura M, Sasaki A, Otani Y, Nakamura Y, Nakamura T, Kuramochi K, Imai T, Kubo N, Okamoto S. Methylthioacetic acid, a derivative of aroma compounds from Cucumis melo var. conomon dose-dependently triggers differentiation and apoptosis of RCM-1 human colorectal cancer cells. J Toxicol Sci. 2023;48(1):25-35. doi: 10.2131/jts.48.25. PMID: 36599425. 7: Sun L, Ren X, Wang IC, Pradhan A, Zhang Y, Flood HM, Han B, Whitsett JA, Kalin TV, Kalinichenko VV. The FOXM1 inhibitor RCM-1 suppresses goblet cell metaplasia and prevents IL-13 and STAT6 signaling in allergen-exposed mice. Sci Signal. 2017 Apr 18;10(475):eaai8583. doi: 10.1126/scisignal.aai8583. PMID: 28420758. 8: Zhang S, Wang J, Hu W, He L, Tang Q, Li J, Jie M, Li X, Liu C, Ouyang Q, Yang S, Hu C. RNF112-mediated FOXM1 ubiquitination suppresses the proliferation and invasion of gastric cancer. JCI Insight. 2023 Jun 8;8(11):e166698. doi: 10.1172/jci.insight.166698. PMID: 37288663; PMCID: PMC10259508. 9: Liu X, Li H, Liu Q, Niu Y, Hu Q, Deng H, Cha J, Wang Y, Liu Y, He Q. Role for Protein Kinase A in the Neurospora Circadian Clock by Regulating White Collar- Independent frequency Transcription through Phosphorylation of RCM-1. Mol Cell Biol. 2015 Jun;35(12):2088-102. doi: 10.1128/MCB.00709-14. Epub 2015 Apr 6. PMID: 25848091; PMCID: PMC4438235. 10: Yang Z, Wang H, Chang S, Cui J, Zhou L, Lv Q, He Y, Du X, Dong J, Ma C. Left ventricular strain-curve morphology to distinguish between constrictive pericarditis and restrictive cardiomyopathy. ESC Heart Fail. 2021 Dec;8(6):4863-4872. doi: 10.1002/ehf2.13679. Epub 2021 Oct 29. PMID: 34713619; PMCID: PMC8712811. 11: Awoyera PO, Odutuga OL, Effiong JU, De Jesus Silvera Sarmiento A, Mortazavi SJ, Hu JW. Development of Fibre-Reinforced Cementitious Mortar with Mineral Wool and Coconut Fibre. Materials (Basel). 2022 Jun 27;15(13):4520. doi: 10.3390/ma15134520. PMID: 35806645; PMCID: PMC9267836. 12: Soongswang J, Sangtawesin C, Sittiwangkul R, Wanitkun S, Muangmingsuk S, Sopontammarak S, Klungratana C, Kangkagate C. Myocardial diseases in Thai children. J Med Assoc Thai. 2002 Aug;85 Suppl 2:S648-57. PMID: 12403243. 13: Kataoka H, Nabeshima K, Komada N, Koono M. New human colorectal carcinoma cell lines that secrete proteinase inhibitors in vitro. Virchows Arch B Cell Pathol Incl Mol Pathol. 1989;57(3):157-65. doi: 10.1007/BF02899077. PMID: 2570482. 14: Karashima S, Kataoka H, Itoh H, Maruyama R, Koono M. Prognostic significance of alpha-1-antitrypsin in early stage of colorectal carcinomas. Int J Cancer. 1990 Feb 15;45(2):244-50. doi: 10.1002/ijc.2910450207. PMID: 2303291. 15: Koller S, Inzinger M, Rothmund M, Ahlgrimm-Siess V, Massone C, Arzberger E, Wolf P, Hofmann-Wellenhof R. UV-induced alterations of the skin evaluated over time by reflectance confocal microscopy. J Eur Acad Dermatol Venereol. 2014 Aug;28(8):1061-8. doi: 10.1111/jdv.12284. Epub 2013 Oct 17. PMID: 24131408. 16: Kataoka H, Maruyama R, Ishihara A, Koono M. Neutral proteinases and inhibitors secreted by human rectal adenocarcinoma cell line (RCM-1). Invasion Metastasis. 1989;9(3):149-66. PMID: 2656568. 17: Komada N, Nabeshima K, Koita H, Kataoka H, Muraoka K, Koono M. Characteristics of a metastatic variant to the liver of human rectal adenocarcinoma cell line RCM-1. Invasion Metastasis. 1993;13(1):38-49. PMID: 8407210. 18: De Sousa PA, Tye BJ, Sneddon S, Bruce K, Dand P, Russell G, Collins DM, Greenshields A, McDonald K, Bradburn H, Gardner J, Downie JM, Courtney A, Brison DR. Derivation of the human embryonic stem cell line RCM1. Stem Cell Res. 2016 Mar;16(2):476-80. doi: 10.1016/j.scr.2015.12.020. Epub 2015 Dec 31. PMID: 27346018. 19: Legeai C, Coutance G, Cantrelle C, Jasseron C, Para M, Sebbag L, Battistella P, Kerbaul F, Dorent R. Waitlist Outcomes in Candidates With Rare Causes of Heart Failure After Implementation of the 2018 French Heart Allocation Scheme. Circ Heart Fail. 2024 Feb;17(2):e010837. doi: 10.1161/CIRCHEARTFAILURE.123.010837. Epub 2024 Feb 1. PMID: 38299331. 20: Shimao Y, Nabeshima K, Inoue T, Koono M. TPA-enhanced motility and invasion in a highly metastatic variant (L-10) of human rectal adenocarcinoma cell line RCM-1: selective role of PKC-alpha and its inhibition by a combination of PDBu- induced PKC downregulation and antisense oligonucleotides treatment. Clin Exp Metastasis. 1999 Jun;17(4):351-60. doi: 10.1023/a:1006696331420. PMID: 10545022.