Anisomycin | CAS#22862-76-6 | peptidyl transferase inhibitor | protein translation inhibitor

MedKoo Cat#: 540022 | Name: Anisomycin

Featured

Description:

WARNING: This product is for research use only, not for human or veterinary use.

Anisomycin, also known as Flagecidin, NSC-76712 and AI3-50846, is a peptidyl transferase and protein translation inhibitor. It inhibits memory consolidation and reconsolidation, induces apoptosis in breast cancer cells, and suppresses proliferation of Jurkat T-cells.

Chemical Structure

Anisomycin

CAS#22862-76-6

Theoretical Analysis

MedKoo Cat#: 540022

Name: Anisomycin

CAS#: 22862-76-6

Chemical Formula: C14H19NO4

Exact Mass: 265.1314

Molecular Weight: 265.30

Elemental Analysis: C, 63.38; H, 7.22; N, 5.28; O, 24.12

Price and Availability

Size	Price	Availability
10mg	USD 250.00	2 Weeks
50mg	USD 550.00	2 Weeks
100mg	USD 850.00	2 Weeks

Bulk Inquiry

Buy Now

Add to Cart

Related CAS #

No Data

Synonym

Flagecidin; NSC-76712; NSC76712; NSC 76712; AI3-50846; AI350846; AI3 50846; Wuningmeisu C

IUPAC/Chemical Name

(2R,3S,4S)-4-hydroxy-2-(4-methoxybenzyl)pyrrolidin-3-yl acetate

InChi Key

YKJYKKNCCRKFSL-RDBSUJKOSA-N

InChi Code

InChI=1S/C14H19NO4/c1-9(16)19-14-12(15-8-13(14)17)7-10-3-5-11(18-2)6-4-10/h3-6,12-15,17H,7-8H2,1-2H3/t12-,13+,14+/m1/s1

SMILES Code

O[C@@H]1[C@@H](OC(C)=O)[C@@H](CC2=CC=C(OC)C=C2)NC1

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

Anisomycin is a JNK activator, which increases phospho-JNK.

In vitro activity:

The effect of different concentrations (1-100 mM) of anisomycin was next analysed on the proliferation of CD44+/CD133+ HuOCSCs in vitro. The MTT assay results demonstrated that after 36 h of cell treatment, the inhibition rate of cell proliferation increased significantly with the increase of anisomycin concentration (Fig. 3A). The results showed that anisomycin caused inhibition of cell proliferation in a dose-dependent manner (IC50=31.8 mM). Flow cytometry analysis of Annexin V/PI double staining indicated that treatment of HuOCSCs with anisomycin for 36 h significantly increased the proportion of apoptotic cells in HuOCSCs, and significantly decreased the proportion of viable cells (Fig. 3B and C). In addition, Transwell invasion assays demonstrated that treatment of HuOCSCs with anisomycin for 36 h significantly weakened their invasive ability in Matrigel (Fig. 4A). Reference: Int J Oncol. 2019 Dec; 55(6): 1296–1312. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6831202/

In vivo activity:

At 1 dpt, the concentration of anisomycin on the percent of hair cells inhibited was highly significant (p < 0.0001). Compared with DMSO group at 1 dpt, about 14.5% hair cells were reduced in 10 μM group, about 58.8% hair cells were reduced in 50 μM group, nearly all hair cell disappeared in 100 μM group. And percent of hair cells inhibited in 10 μM and 50 μM treatment group increased along with treatment duration (Fig. 1B). These results indicate that anisomycin exposure can induce hair cells loss in zebrafish posterior lateral line neuromast in a time- and dose-dependent manner. Reference: Comp Biochem Physiol C Toxicol Pharmacol. 2021 Sep;247:109053. https://pubmed.ncbi.nlm.nih.gov/33887477/

	Solvent	mg/mL	mM
Solubility
	DMSO	37.4	140.90
	DMF	20.0	75.39
	Ethanol	22.4	84.51
	PBS (pH 7.2)	0.5	1.88

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 265.30 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Ye W, Ni Z, Yicheng S, Pan H, Huang Y, Xiong Y, Liu T. Anisomycin inhibits angiogenesis in ovarian cancer by attenuating the molecular sponge effect of the lncRNA‑Meg3/miR‑421/PDGFRA axis. Int J Oncol. 2019 Dec;55(6):1296-1312. doi: 10.3892/ijo.2019.4887. Epub 2019 Sep 30. PMID: 31638182; PMCID: PMC6831202. 2. Li Y, Hu J, Song H, Wu T. Antibiotic anisomycin selectively targets leukemia cell lines and patient samples through suppressing Wnt/β-catenin signaling. Biochem Biophys Res Commun. 2018 Nov 2;505(3):858-864. doi: 10.1016/j.bbrc.2018.09.183. Epub 2018 Oct 6. PMID: 30301525. 3. Yuan X, Qin Y, Wang J, Fan C. Anisomycin induces hair cell death and blocks supporting cell proliferation in zebrafish lateral line neuromast. Comp Biochem Physiol C Toxicol Pharmacol. 2021 Sep;247:109053. doi: 10.1016/j.cbpc.2021.109053. Epub 2021 Apr 19. PMID: 33887477. 4. Quintana VM, Selisko B, Brunetti JE, Eydoux C, Guillemot JC, Canard B, Damonte EB, Julander JG, Castilla V. Antiviral activity of the natural alkaloid anisomycin against dengue and Zika viruses. Antiviral Res. 2020 Apr;176:104749. doi: 10.1016/j.antiviral.2020.104749. Epub 2020 Feb 17. PMID: 32081740; PMCID: PMC7279513.

In vitro protocol:

In vivo protocol:

1. Yuan X, Qin Y, Wang J, Fan C. Anisomycin induces hair cell death and blocks supporting cell proliferation in zebrafish lateral line neuromast. Comp Biochem Physiol C Toxicol Pharmacol. 2021 Sep;247:109053. doi: 10.1016/j.cbpc.2021.109053. Epub 2021 Apr 19. PMID: 33887477. 2. Quintana VM, Selisko B, Brunetti JE, Eydoux C, Guillemot JC, Canard B, Damonte EB, Julander JG, Castilla V. Antiviral activity of the natural alkaloid anisomycin against dengue and Zika viruses. Antiviral Res. 2020 Apr;176:104749. doi: 10.1016/j.antiviral.2020.104749. Epub 2020 Feb 17. PMID: 32081740; PMCID: PMC7279513.

1: Hu R, Ma Q, Kong Y, Wang Z, Xu M, Chen X, Su Y, Xiao T, He Q, Wang X, Xu W, Yang Y, Wang X, Li X, Liu Y, Chen S, Zhao R, Guo M, Wang G, Li W. A Compound Screen Based on Isogenic hESC-Derived β Cell Reveals an Inhibitor Targeting ZnT8-Mediated Zinc Transportation to Protect Pancreatic β Cell from Stress- Induced Cell Death. Adv Sci (Weinh). 2025 Apr 7:e2413161. doi: 10.1002/advs.202413161. Epub ahead of print. PMID: 40192532. 2: Zhao L, Wu X, Li Q, Shen Y, Zeng S, Wang J, Liu Q. Inhibition of CCN5 Protects Against Apoptosis and Endoplasmic Reticulum Stress in Bisphenol A-Induced Sertoli Cells via p38/JNK MAPK Signaling Pathway. DNA Cell Biol. 2025 Apr;44(4):174-185. doi: 10.1089/dna.2024.0247. Epub 2025 Feb 26. PMID: 40184555. 3: Li Z, Chen R, Tang W, Zheng X, Jin X, Wang Z, Wu Q. Sheng-Jiang-Yi-You decoction inhibits the inflammatory response by down-regulating the p38MAPK signaling pathway to alleviate Helicobacter pylori-associated gastritis. Histol Histopathol. 2025 Mar 17:18906. doi: 10.14670/HH-18-906. Epub ahead of print. PMID: 40159952. 4: Bao R, Li H, Li S, Su M, Li W. Benfuracarb impairs zebrafish swim bladder development via the JNK2 pathway mediated inhibition of autophagy. Pestic Biochem Physiol. 2025 Apr;209:106329. doi: 10.1016/j.pestbp.2025.106329. Epub 2025 Feb 13. PMID: 40082026. 5: Eom YS, Kim SJ. Regulatory dynamics of Nanog in chondrocyte dedifferentiation: role of KLF4/p53 and p38/AKT signaling. Funct Integr Genomics. 2025 Mar 11;25(1):58. doi: 10.1007/s10142-025-01572-7. PMID: 40067510. 6: Kinsky NR, Orlin DJ, Ruesch EA, Kim B, Coello S, Diba K, Ramirez S. Erasable hippocampal neural signatures predict memory discrimination. Cell Rep. 2025 Mar 25;44(3):115391. doi: 10.1016/j.celrep.2025.115391. Epub 2025 Mar 8. PMID: 40057951. 7: Hu M, Li C, Sun Q, Hu B, Yang J, Wang X, Huang J, Shen D. The role of the PKCζ/JNK signaling pathway in regulating the development of femoral head necrosis. Braz J Med Biol Res. 2025 Mar 3;58:e13771. doi: 10.1590/1414-431X2025e13771. PMID: 40053032; PMCID: PMC11884774. 8: Zhang CY, Fan C, Li SH, Wu J, Peng YZ, Sung HW, Liu S, Li RK. A conductive polymer restores connexin43 expression through the suppression of mitogen- activated protein kinases to improve intercellular communication and alleviate atrial fibrillation. Acta Biomater. 2025 Apr;196:123-135. doi: 10.1016/j.actbio.2025.02.058. Epub 2025 Feb 27. PMID: 40023467. 9: Li S, Chen M, Zheng S, Abudourexiti W, Zhu F, Wang Z, Guo Y, Yu Z, Yang Z, Zhang L, Ding C, Gong J. ZAKα Induces Pyroptosis of Colonic Epithelium Via the Caspase-11/GSDMD Pathway to Aggravate Colitis. Inflammation. 2025 Feb 24. doi: 10.1007/s10753-025-02262-z. Epub ahead of print. PMID: 39992609. 10: Wang J, Xu C, Xiao W, Wang M, Han X, Sun Y, Ge B. The fermentation broth of Streptomyces noursei strain S86 as a potential biocontrol product for Fusarium crown rot of wheat. Plant Dis. 2025 Feb 19. doi: 10.1094/PDIS-10-24-2109-RE. Epub ahead of print. PMID: 39970104. 11: Song Y, Zhang X, Han X, Wang G, Wang M, Wu H, Wu X. Ginsenoside Rb1 alleviates blood-brain barrier damage and demyelination in experimental autoimmune encephalomyelitis mice by regulating JNK/ ERK/NF-κB signaling pathway. J Ethnopharmacol. 2025 Mar 13;343:119448. doi: 10.1016/j.jep.2025.119448. Epub 2025 Feb 4. PMID: 39914687. 12: Fan B, Yin L, Wang A', Li F, Han S. PIM1 enhances insulin secretion and inhibits ferroptosis of high glucose-induced pancreatic β-cells through strengthening PINK1/Parkin-mediated mitophagy via inactivating JNK/p38 signaling pathway. Tissue Cell. 2025 Apr;93:102722. doi: 10.1016/j.tice.2025.102722. Epub 2025 Jan 7. PMID: 39813743. 13: Ha YS, Kim TK, Heo J, Oh J, Kim SK, Kim J, Lee J, Yang SR, Hwang S, Kim SJ. Rocaglamide-A mitigates LPS-induced hepatic inflammation by modulating JNK/AP-1 signaling cascade and ROS production in hepatocytes. Toxicol Res. 2024 Sep 27;41(1):47-59. doi: 10.1007/s43188-024-00263-y. PMID: 39802115; PMCID: PMC11717754. 14: Xu S, Wu Y, Wang Y, Li Y, He Y, Ren L, Zheng Y, Chen P, Zhao JA, Ye J, Meng F, Li T, Yu Y. Huanglian Ganjiang Decoction alleviates DSS-induced colitis through suppressing inflammation and protecting intestinal barrier: From the perspective of disassembling prescriptions. J Ethnopharmacol. 2025 Feb 11;341:119340. doi: 10.1016/j.jep.2025.119340. Epub 2025 Jan 7. PMID: 39788168. 15: Costa ALO, Dos Santos M, Dantas-Vieira GC, Lopes REN, Vommaro RC, Martins- Duarte ÉS. Antiproliferative and Morphological Analysis Triggered by Drugs Contained in the Medicines for Malaria Venture COVID-Box Against Toxoplasma gondii Tachyzoites. Microorganisms. 2024 Dec 16;12(12):2602. doi: 10.3390/microorganisms12122602. PMID: 39770804; PMCID: PMC11676817. 16: Ma D, Du H, Huang Y, Pan A, Gan L. Study of the effect of azithromycin on airway remodeling in asthma via the SAPK/JNK pathway. J Cardiothorac Surg. 2024 Dec 30;19(1):687. doi: 10.1186/s13019-024-03193-w. PMID: 39736750; PMCID: PMC11684290. 17: Wang CM, Wu YD, Liang SL, Gao S, Yue ZL, Kong LM, Gao KH, Li NH. [Mechanism of kaempferol on intervertebral disc degeneration based on p38 MAPK signaling pathway]. Zhongguo Zhong Yao Za Zhi. 2024 Nov;49(21):5721-5729. Chinese. doi: 10.19540/j.cnki.cjcmm.20240625.401. PMID: 39701783. 18: Xu H, Zhou Z, Wen F, Sun H, Hou J. Inhibiting autophagy further promotes Ginkgolide B's anti-osteoclastogenesis ability. Bone. 2025 Mar;192:117348. doi: 10.1016/j.bone.2024.117348. Epub 2024 Nov 29. PMID: 39617111. 19: Sherawat K, Mehan S, Khan Z, Tiwari A, Gupta GD, Narula AS. Neuroprotective Potential of Tanshinone-IIA in Mitigating Propionic Acidinduced Experimental Autism-like Behavioral and Neurochemical Alterations: Insights into c-JNK and p38MAPK Pathways. Curr Mol Pharmacol. 2024;17:e18761429326799. doi: 10.2174/0118761429326799241121104310. PMID: 39604159. 20: Peng LT, Li MW, Song ZJ, Li Q, Zhan KB, Yan CQ, Ling HY. Dihydromyricetin ameliorates neurotoxicity induced by high glucose through restraining ferroptosis by inhibiting JNK-inflammation pathway in HT22 cells. Neuroscience. 2025 Jan 26;565:40-51. doi: 10.1016/j.neuroscience.2024.11.061. Epub 2024 Nov 26. PMID: 39603402.