MJN228 | CAS#459168-97-9

MedKoo Cat#: 532184 | Name: MJN228

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

MJN228 is a selective ligand for the lipid-binding protein nucleobindin 1 (NUCB1), blocking binding of the probe arachidonoyl ethanolamide-diazirine with an IC50 value of 3.3 µM. It prevents probe binding to NUCB1 in Neuro2a cells at concentrations as low as 10 µM.

Chemical Structure

MJN228

CAS#459168-97-9

Theoretical Analysis

MedKoo Cat#: 532184

Name: MJN228

CAS#: 459168-97-9

Chemical Formula: C20H20N4O3

Exact Mass: 364.1535

Molecular Weight: 364.41

Elemental Analysis: C, 65.92; H, 5.53; N, 15.38; O, 13.17

Price and Availability

Size	Price	Availability	Quantity
5mg	USD 550.00	2 weeks
10mg	USD 1,000.00	2 weeks

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Related CAS #

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Synonym

MJN228; MJN 228; MJN-228.

IUPAC/Chemical Name

(4-methylpiperazin-1-yl)-(5-nitro-3-phenyl-1H-indol-2-yl)methanone

InChi Key

BLSWPPPBUQFKHH-UHFFFAOYSA-N

InChi Code

InChI=1S/C20H20N4O3/c1-22-9-11-23(12-10-22)20(25)19-18(14-5-3-2-4-6-14)16-13-15(24(26)27)7-8-17(16)21-19/h2-8,13,21H,9-12H2,1H3

SMILES Code

O=C(N1CCN(C)CC1)C(N2)=C(C3=CC=CC=C3)C4=C2C=CC([N+]([O-])=O)=C4

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

MJN228 is a lipid-binding protein nucleobindin-1 (NUCB1) inhibitor with an IC50 of 3.3 μM.

In vitro activity:

Treatment of Neuro2a cells with MJN228 or KML110 (25 μM) produced substantial (∼3-5-fold) reductions in lipid probe enrichment of NUCB1, while KML181 had no effect (Figure 7A). MJN228 inhibited lipid probe binding to NUCB1 at concentrations as low as 10 μM, with near-maximal inhibition observed at ∼25 μM (Figure S7A). Reference: Cell. 2015 Jun 18;161(7):1668-80. https://pubmed.ncbi.nlm.nih.gov/26091042/

In vivo activity:

TBD

	Solvent	mg/mL	mM
Solubility
	DMF	30.0	82.33
	DMF:PBS (pH 7.2) (1:3)	0.3	0.69
	DMSO	25.0	68.60

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 364.41 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

Niphakis MJ, Lum KM, Cognetta AB 3rd, Correia BE, Ichu TA, Olucha J, Brown SJ, Kundu S, Piscitelli F, Rosen H, Cravatt BF. A Global Map of Lipid-Binding Proteins and Their Ligandability in Cells. Cell. 2015 Jun 18;161(7):1668-80. doi: 10.1016/j.cell.2015.05.045. PMID: 26091042; PMCID: PMC4615699.

In vitro protocol:

In vivo protocol:

TBD

1. M. J. Niphakis, K. M. Lum, A. B. Cognetta, III, et al. A global map of the lipid-binding proteins and their ligandability in cells. Cell 161, 1668-1680 (2015).