JWH015 | CAS# 155471-08-2 | CB2 agonist

MedKoo Cat#: 531991 | Name: JWH015

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

JWH015 is a selective CB2 agonist (Ki values are 13.8 and 383 nM as measured at human cloned CB2 and CB1 receptors expressed in CHO cells).

Chemical Structure

JWH015

CAS# 155471-08-2

Theoretical Analysis

MedKoo Cat#: 531991

Name: JWH015

CAS#: 155471-08-2

Chemical Formula: C23H21NO

Exact Mass: 327.1623

Molecular Weight: 327.43

Elemental Analysis: C, 84.37; H, 6.46; N, 4.28; O, 4.89

Price and Availability

Size	Price	Availability	Quantity
5mg	USD 320.00	2 Weeks
10mg	USD 550.00	2 Weeks

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Related CAS #

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Synonym

JWH015; JWH 015; JWH-015.

IUPAC/Chemical Name

(2-methyl-1-propylindol-3-yl)-naphthalen-1-ylmethanone

InChi Key

LJSBBBWQTLXQEN-UHFFFAOYSA-N

InChi Code

InChI=1S/C23H21NO/c1-3-15-24-16(2)22(20-12-6-7-14-21(20)24)23(25)19-13-8-10-17-9-4-5-11-18(17)19/h4-14H,3,15H2,1-2H3

SMILES Code

O=C(C1=C(C)N(CCC)C2=C1C=CC=C2)C3=C4C=CC=CC4=CC=C3

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

Selective CB2 agonist.

In vitro activity:

Pretreatment of human RASFs with JWH-015 (10-20 μM) markedly inhibited the ability of pro-inflammatory cytokine interleukin-1β (IL-1β) to induce production of IL-6 and IL-8 and cellular expression of inflammatory cyclooxygenase-2 (COX-2). Reference: Front Immunol. 2019 May 8;10:1027. https://pubmed.ncbi.nlm.nih.gov/31139184/

In vivo activity:

Therefore, the present study aimed to determine whether the analgesic effects of the selective CB2 agonist JWH015 was mediated by the activation of autophagy in BCP (bone cancer pain). BCP was induced by the intra‑femur implantation of NCTC2472 fibrosarcoma cells in C3H/HeN mice. Intrathecal administration of the selective CB2 agonist JWH015 ameliorated autophagy flux through the downregulation of IL‑1β and IL‑6 and attenuated BCP. Reference: Mol Med Rep. 2019 Dec;20(6):5100-5110. https://pubmed.ncbi.nlm.nih.gov/31661120/

	Solvent	mg/mL	mM
Solubility
	DMF	10.0	30.54
	DMSO	10.0	30.54
	Ethanol	10.0	30.54

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 327.43 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Fechtner S, Singh AK, Srivastava I, Szlenk CT, Muench TR, Natesan S, Ahmed S. Cannabinoid Receptor 2 Agonist JWH-015 Inhibits Interleukin-1β-Induced Inflammation in Rheumatoid Arthritis Synovial Fibroblasts and in Adjuvant Induced Arthritis Rat via Glucocorticoid Receptor. Front Immunol. 2019 May 8;10:1027. doi: 10.3389/fimmu.2019.01027. PMID: 31139184; PMCID: PMC6519139. 2. Bort A, Alvarado-Vazquez PA, Moracho-Vilrriales C, Virga KG, Gumina G, Romero-Sandoval A, Asbill S. Effects of JWH015 in cytokine secretion in primary human keratinocytes and fibroblasts and its suitability for topical/transdermal delivery. Mol Pain. 2017 Jan;13:1744806916688220. doi: 10.1177/1744806916688220. PMID: 28326930; PMCID: PMC5302180. 3. Mao Y, Huang Y, Zhang Y, Wang C, Wu H, Tian X, Liu Y, Hou B, Liang Y, Rong H, Gu X, Ma Z. Cannabinoid receptor 2‑selective agonist JWH015 attenuates bone cancer pain through the amelioration of impaired autophagy flux induced by inflammatory mediators in the spinal cord. Mol Med Rep. 2019 Dec;20(6):5100-5110. doi: 10.3892/mmr.2019.10772. Epub 2019 Oct 25. PMID: 31661120; PMCID: PMC6854597. 4. Craft RM, Greene NZ, Wakley AA. Antinociceptive effects of JWH015 in female and male rats. Behav Pharmacol. 2018 Apr;29(2 and 3-Spec Issue):280-289. doi: 10.1097/FBP.0000000000000337. PMID: 28914627; PMCID: PMC5849479.

In vitro protocol:

In vivo protocol:

1. Mao Y, Huang Y, Zhang Y, Wang C, Wu H, Tian X, Liu Y, Hou B, Liang Y, Rong H, Gu X, Ma Z. Cannabinoid receptor 2‑selective agonist JWH015 attenuates bone cancer pain through the amelioration of impaired autophagy flux induced by inflammatory mediators in the spinal cord. Mol Med Rep. 2019 Dec;20(6):5100-5110. doi: 10.3892/mmr.2019.10772. Epub 2019 Oct 25. PMID: 31661120; PMCID: PMC6854597. 2. Craft RM, Greene NZ, Wakley AA. Antinociceptive effects of JWH015 in female and male rats. Behav Pharmacol. 2018 Apr;29(2 and 3-Spec Issue):280-289. doi: 10.1097/FBP.0000000000000337. PMID: 28914627; PMCID: PMC5849479.

1: Sun Y, Zhang W, Liu Y, Liu X, Ma Z, Gu X. Intrathecal injection of JWH015 attenuates remifentanil-induced postoperative hyperalgesia by inhibiting activation of spinal glia in a rat model. Anesth Analg. 2014 Apr;118(4):841-53. doi: 10.1213/ANE.0000000000000146. PubMed PMID: 24651239. 2: Murataeva N, Mackie K, Straiker A. The CB2-preferring agonist JWH015 also potently and efficaciously activates CB1 in autaptic hippocampal neurons. Pharmacol Res. 2012 Nov;66(5):437-42. doi: 10.1016/j.phrs.2012.08.002. PubMed PMID: 22921769; PubMed Central PMCID: PMC3601544. 3: Gu X, Mei F, Liu Y, Zhang R, Zhang J, Ma Z. Intrathecal administration of the cannabinoid 2 receptor agonist JWH015 can attenuate cancer pain and decrease mRNA expression of the 2B subunit of N-methyl-D-aspartic acid. Anesth Analg. 2011 Aug;113(2):405-11. doi: 10.1213/ANE.0b013e31821d1062. PubMed PMID: 21519057. 4: Zhong L, Geng L, Njie Y, Feng W, Song ZH. CB2 cannabinoid receptors in trabecular meshwork cells mediate JWH015-induced enhancement of aqueous humor outflow facility. Invest Ophthalmol Vis Sci. 2005 Jun;46(6):1988-92. PubMed PMID: 15914613.