NSC 632839 hydrochloride | CAS#157654-67-6 | ubiquitin isopeptidase inhibitor

MedKoo Cat#: 530392 | Name: NSC-632839 hydrochloride

Description:

WARNING: This product is for research use only, not for human or veterinary use.

NSC 632839 is an Inhibitor of ubiquitin isopeptidase activity.

Chemical Structure

NSC-632839 hydrochloride

CAS#157654-67-6 (HCl)

Theoretical Analysis

MedKoo Cat#: 530392

Name: NSC-632839 hydrochloride

CAS#: 157654-67-6 (HCl)

Chemical Formula: C21H22ClNO

Exact Mass: 0.0000

Molecular Weight: 339.86

Elemental Analysis: C, 74.22; H, 6.52; Cl, 10.43; N, 4.12; O, 4.71

Price and Availability

Size	Price	Availability	Quantity
1g	USD 2,150.00	4 Weeks
2g	USD 3,650.00	4 Weeks

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Related CAS #

No Data

Synonym

NSC-632839; NSC 632839; NSC632839; NSC 632839 hydrochloride

IUPAC/Chemical Name

3,5-Bis[(4-methylphenyl)methylene]-4-piperidone hydrochloride

InChi Key

ZOKZLTXPTLIWOJ-BYCVLTJGSA-N

InChi Code

InChI=1S/C21H21NO.ClH/c1-15-3-7-17(8-4-15)11-19-13-22-14-20(21(19)23)12-18-9-5-16(2)6-10-18;/h3-12,22H,13-14H2,1-2H3;1H/b19-11+,20-12+;

SMILES Code

O=C1/C(CNC/C1=C\C2=CC=C(C)C=C2)=C/C3=CC=C(C)C=C3.[H]Cl

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

NSC632839 is a nonselective isopeptidase inhibitor, which inhibits USP2, USP7, and SENP2 with EC50s of 45±4 μM, 37±1 μM, and 9.8±1.8 μM.

In vitro activity:

To further characterize NSC 632839 against purified enzymes, this study determined its inhibitory potential against purified USP2, USP7, and SENP2 and demonstrated that NSC 632839 was not only a DUB inhibitor, but also a deSUMOylase inhibitor (Fig. 5A–C). Specifically, NSC 632839 inhibited USP2, USP7, and SENP2 with EC50 values of 45 ± 4 μM, 37 ± 1 μM, and 9.8 ± 1.8 μM, respectively. Importantly, NSC 632839 did not inhibit the reporter enzyme PLA2 over the concentration range tested (1.2–150 μM), indicating that the reported inhibition was selective for isopeptidases (Fig. 5D). Reference: Protein Sci. 2008 Jun;17(6):1035-43. https://pubmed.ncbi.nlm.nih.gov/18424514/

In vivo activity:

TBD

	Solvent	mg/mL	mM
Solubility
	DMF	10.0	29.42
	DMSO	7.7	22.75
	Ethanol	0.3	0.74

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 339.86 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

Nicholson B, Leach CA, Goldenberg SJ, Francis DM, Kodrasov MP, Tian X, Shanks J, Sterner DE, Bernal A, Mattern MR, Wilkinson KD, Butt TR. Characterization of ubiquitin and ubiquitin-like-protein isopeptidase activities. Protein Sci. 2008 Jun;17(6):1035-43. doi: 10.1110/ps.083450408. Epub 2008 Apr 18. PMID: 18424514; PMCID: PMC2386736.

In vitro protocol:

In vivo protocol:

TBD

1: Nicholson B, Leach CA, Goldenberg SJ, Francis DM, Kodrasov MP, Tian X, Shanks J, Sterner DE, Bernal A, Mattern MR, Wilkinson KD, Butt TR. Characterization of ubiquitin and ubiquitin-like-protein isopeptidase activities. Protein Sci. 2008 Jun;17(6):1035-43. doi: 10.1110/ps.083450408. PubMed PMID: 18424514; PubMed Central PMCID: PMC2386736. 2: Aleo E, Henderson CJ, Fontanini A, Solazzo B, Brancolini C. Identification of new compounds that trigger apoptosome-independent caspase activation and apoptosis. Cancer Res. 2006 Sep 15;66(18):9235-44. PubMed PMID: 16982768.