JT010 is a potent and TRPA1-selective channel activator via covalent modification of TRPA1 active site Cys621. JT010 opens the TRPA1 channel by covalently and site-selectively binding to Cys621 (EC50 = 0.65 nM). The results suggest that a single modification of Cys621 is sufficient to open the TRPA1 channel. The TRPA1-selective probe described herein might be useful for further mechanistic studies of TRPA1 activation.
MedKoo Cat#: 530362
Name: JT010
CAS#: 917562-33-5
Chemical Formula: C16H19ClN2O3S
Exact Mass: 354.0805
Molecular Weight: 354.85
Elemental Analysis: C, 54.16; H, 5.40; Cl, 9.99; N, 7.89; O, 13.53; S, 9.03
| Solvent | mg/mL | mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 69.0 | 194.30 | |
| Ethanol | 26.9 | 75.72 |
The following data is based on the product molecular weight 354.85 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
