MedKoo Biosciences, Inc.
Tel:   +1-919-636-5577    Fax:   +1-919-980-4831    Email:   sales@medkoo.com
Search
Login Register
Search
MedKoo Cat#: 530375 | Name: MQAE
Featured New

Description:

WARNING: This product is for research use only, not for human or veterinary use.

MQAE is a fluorescent indicator dye that can be used to measure intracellular and extracellular chloride concentrations (absorption/emission max: 350/460 nm).1 It detects the ion via diffusion-limited collisional quenching.

Chemical Structure

MQAE
MQAE
CAS#162558-52-3

Theoretical Analysis

MedKoo Cat#: 530375

Name: MQAE

CAS#: 162558-52-3

Chemical Formula: C14H16BrNO3

Exact Mass: 0.0000

Molecular Weight: 326.19

Elemental Analysis: C, 51.55; H, 4.94; Br, 24.50; N, 4.29; O, 14.71

Price and Availability

Size Price Availability Quantity
500mg USD 750.00 2 Weeks
1g USD 1,250.00 2 Weeks
2g USD 2,250.00 2 Weeks
Bulk Inquiry
Buy Now
Add to Cart
Related CAS #
No Data
Synonym
MQAE
IUPAC/Chemical Name
1-(2-ethoxy-2-oxoethyl)-6-methoxy-quinolinium, monobromide
InChi Key
DSLLHVISNOIYHR-UHFFFAOYSA-M
InChi Code
InChI=1S/C14H16NO3.BrH/c1-3-18-14(16)10-15-8-4-5-11-9-12(17-2)6-7-13(11)15;/h4-9H,3,10H2,1-2H3;1H/q+1;/p-1
SMILES Code
COC1=CC2=CC=C[N+](CC(OCC)=O)=C2C=C1.[Br-]
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
MQAE is a novel chloride ion fluorescence probe excited by ultraviolet light.
In vitro activity:
The present study compares two methods for studies of chloride transport: X-ray microanalysis and MQAE fluorescence with image analysis. This study shows that X-ray microanalysis and MQAE fluorescence are adequate and comparable methods for measuring cAMP-dependent chloride transport in individual cells. Reference: Microsc Res Tech. 2002 Dec 15;59(6):531-5. https://pubmed.ncbi.nlm.nih.gov/12467030/
In vivo activity:
In an attempt to elucidate the effect or lack of effect of cell volume on MQAE concentration, we studied the effects of changes in cell volume, achieved by applying different levels of osmotic stress, on the intensity of MQAE fluorescence in mouse airway ciliary cells. The intracellular distribution of MQAEs was heterogeneous and not affected by the changes in osmotic stress-induced cell volume, suggesting that MQAEs are bound to un-identified subcellular structures. These bound MQAEs appear to have enabled the measurement of [Cl-]i in airway ciliary cells, even under conditions of cell volume change. Reference: J Physiol Sci. 2018 Mar;68(2):191-199. https://pubmed.ncbi.nlm.nih.gov/29332212/
Solvent mg/mL mM
Solubility
DMF 12.0 36.79
DMSO 40.0 122.63
Ethanol 40.5 124.16
PBS (pH 7.2) 10.0 30.66
Water 82.5 252.92
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 326.19 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Andersson C, Roomans GM. Determination of chloride efflux by X-ray microanalysis versus MQAE-fluorescence. Microsc Res Tech. 2002 Dec 15;59(6):531-5. doi: 10.1002/jemt.10234. PMID: 12467030. 2. Koncz C, Daugirdas JT. Use of MQAE for measurement of intracellular [Cl-] in cultured aortic smooth muscle cells. Am J Physiol. 1994 Dec;267(6 Pt 2):H2114-23. doi: 10.1152/ajpheart.1994.267.6.H2114. PMID: 7810712. 3. Ikeuchi Y, Kogiso H, Hosogi S, Tanaka S, Shimamoto C, Inui T, Nakahari T, Marunaka Y. Measurement of [Cl-]i unaffected by the cell volume change using MQAE-based two-photon microscopy in airway ciliary cells of mice. J Physiol Sci. 2018 Mar;68(2):191-199. doi: 10.1007/s12576-018-0591-y. Epub 2018 Jan 13. PMID: 29332212. 4. Kovalchuk Y, Garaschuk O. Two-photon chloride imaging using MQAE in vitro and in vivo. Cold Spring Harb Protoc. 2012 Jul 1;2012(7):778-85. doi: 10.1101/pdb.prot070037. PMID: 22753606.
In vitro protocol:
1. Andersson C, Roomans GM. Determination of chloride efflux by X-ray microanalysis versus MQAE-fluorescence. Microsc Res Tech. 2002 Dec 15;59(6):531-5. doi: 10.1002/jemt.10234. PMID: 12467030. 2. Koncz C, Daugirdas JT. Use of MQAE for measurement of intracellular [Cl-] in cultured aortic smooth muscle cells. Am J Physiol. 1994 Dec;267(6 Pt 2):H2114-23. doi: 10.1152/ajpheart.1994.267.6.H2114. PMID: 7810712.
In vivo protocol:
1. Ikeuchi Y, Kogiso H, Hosogi S, Tanaka S, Shimamoto C, Inui T, Nakahari T, Marunaka Y. Measurement of [Cl-]i unaffected by the cell volume change using MQAE-based two-photon microscopy in airway ciliary cells of mice. J Physiol Sci. 2018 Mar;68(2):191-199. doi: 10.1007/s12576-018-0591-y. Epub 2018 Jan 13. PMID: 29332212. 2. Kovalchuk Y, Garaschuk O. Two-photon chloride imaging using MQAE in vitro and in vivo. Cold Spring Harb Protoc. 2012 Jul 1;2012(7):778-85. doi: 10.1101/pdb.prot070037. PMID: 22753606.
1: Kovalchuk Y, Garaschuk O. Two-photon chloride imaging using MQAE in vitro and in vivo. Cold Spring Harb Protoc. 2012 Jul 1;2012(7):778-85. doi: 10.1101/pdb.prot070037. PubMed PMID: 22753606. 2: Andersson C, Roomans GM. Determination of chloride efflux by X-ray microanalysis versus MQAE-fluorescence. Microsc Res Tech. 2002 Dec 15;59(6):531-5. PubMed PMID: 12467030. 3: Koncz C, Daugirdas JT. Use of MQAE for measurement of intracellular [Cl-] in cultured aortic smooth muscle cells. Am J Physiol. 1994 Dec;267(6 Pt 2):H2114-23. PubMed PMID: 7810712. 4: Marandi N, Konnerth A, Garaschuk O. Two-photon chloride imaging in neurons of brain slices. Pflugers Arch. 2002 Dec;445(3):357-65. PubMed PMID: 12466938. 5: Liu LI, Cai S, Qiu G, Lin J. Fluid shear stress enhances the cell volume decrease of osteoblast cells by increasing the expression of the ClC-3 chloride channel. Biomed Rep. 2016 Apr;4(4):408-412. PubMed PMID: 27073622; PubMed Central PMCID: PMC4812491. 6: Li YY, Xiao L, Qiu LY, Yan YF, Wang H, Duan GL, Liao ZP, Chen HP. Sasanquasaponin-induced cardioprotection involves inhibition of mPTP opening via attenuating intracellular chloride accumulation. Fitoterapia. 2017 Jan;116:1-9. doi: 10.1016/j.fitote.2016.11.003. PubMed PMID: 27838499. 7: Zheng K, Chen M, Xiang Y, Ma K, Jin F, Wang X, Wang X, Wang S, Wang Y. Inhibition of herpes simplex virus type 1 entry by chloride channel inhibitors tamoxifen and NPPB. Biochem Biophys Res Commun. 2014 Apr 18;446(4):990-6. doi: 10.1016/j.bbrc.2014.03.050. PubMed PMID: 24657267. 8: Gallos G, Remy KE, Danielsson J, Funayama H, Fu XW, Chang HY, Yim P, Xu D, Emala CW Sr. Functional expression of the TMEM16 family of calcium-activated chloride channels in airway smooth muscle. Am J Physiol Lung Cell Mol Physiol. 2013 Nov 1;305(9):L625-34. doi: 10.1152/ajplung.00068.2013. PubMed PMID: 23997176; PubMed Central PMCID: PMC3840275. 9: Keitel V, Häussinger D. TGR5 in the biliary tree. Dig Dis. 2011;29(1):45-7. doi: 10.1159/000324127. Review. PubMed PMID: 21691103. 10: Zhu Y, Parsons SP, Huizinga JD. Measurement of intracellular chloride ion concentration in ICC in situ and in explant culture. Neurogastroenterol Motil. 2010 Jun;22(6):704-9. doi: 10.1111/j.1365-2982.2010.01501.x. Erratum in: Neurogastroenterol Motil. 2010 Sep;22(9):1042. PubMed PMID: 20403100. 11: Untiet V, Kovermann P, Gerkau NJ, Gensch T, Rose CR, Fahlke C. Glutamate transporter-associated anion channels adjust intracellular chloride concentrations during glial maturation. Glia. 2017 Feb;65(2):388-400. doi: 10.1002/glia.23098. PubMed PMID: 27859594. 12: Nakajima KI, Marunaka Y. Intracellular chloride ion concentration in differentiating neuronal cell and its role in growing neurite. Biochem Biophys Res Commun. 2016 Oct 14;479(2):338-342. doi: 10.1016/j.bbrc.2016.09.075. PubMed PMID: 27641671. 13: Munkonge F, Alton EW, Andersson C, Davidson H, Dragomir A, Edelman A, Farley R, Hjelte L, McLachlan G, Stern M, Roomans GM. Measurement of halide efflux from cultured and primary airway epithelial cells using fluorescence indicators. J Cyst Fibros. 2004 Aug;3 Suppl 2:171-6. Review. PubMed PMID: 15463953. 14: Keitel V, Cupisti K, Ullmer C, Knoefel WT, Kubitz R, Häussinger D. The membrane-bound bile acid receptor TGR5 is localized in the epithelium of human gallbladders. Hepatology. 2009 Sep;50(3):861-70. doi: 10.1002/hep.23032. PubMed PMID: 19582812. 15: Hille C, Lahn M, Löhmannsröben HG, Dosche C. Two-photon fluorescence lifetime imaging of intracellular chloride in cockroach salivary glands. Photochem Photobiol Sci. 2009 Mar;8(3):319-27. doi: 10.1039/b813797h. PubMed PMID: 19255672. 16: Kim S, Ma L, Unruh J, McKinney S, Yu CR. Intracellular chloride concentration of the mouse vomeronasal neuron. BMC Neurosci. 2015 Dec 15;16:90. doi: 10.1186/s12868-015-0230-y. PubMed PMID: 26667019; PubMed Central PMCID: PMC4678706. 17: Srinivas SP, Maertens C, Goon LH, Goon L, Satpathy M, Yue BY, Droogmans G, Nilius B. Cell volume response to hyposmotic shock and elevated cAMP in bovine trabecular meshwork cells. Exp Eye Res. 2004 Jan;78(1):15-26. PubMed PMID: 14667824. 18: Stakišaitis D, Meilus V, Juška A, Matusevičius P, Didžiapetrienė J. Sodium is not required for chloride efflux via chloride/bicarbonate exchanger from rat thymic lymphocytes. Biomed Res Int. 2014;2014:569650. doi: 10.1155/2014/569650. PubMed PMID: 25003116; PubMed Central PMCID: PMC4070514. 19: Oliynyk I, Hussain R, Amin A, Johannesson M, Roomans GM. The effect of NO-donors on chloride efflux, intracellular Ca(2+) concentration and mRNA expression of CFTR and ENaC in cystic fibrosis airway epithelial cells. Exp Mol Pathol. 2013 Jun;94(3):474-80. doi: 10.1016/j.yexmp.2013.03.003. PubMed PMID: 23523754. 20: Lin FJ, Yang XS, Yang D, Zou YQ. [Expression of cation-chloride cotransporters KCC2 and NKCC1 in brainstem of para- chlorophenylalanine-induced acute insomnia rats]. Zhonghua Yi Xue Za Zhi. 2013 May 21;93(19):1507-11. Chinese. PubMed PMID: 24029579.