CP21R7 | CAS#125314-13-8 | GSK-3β inhibitor

MedKoo Cat#: 530344 | Name: CP21R7

Featured

Description:

WARNING: This product is for research use only, not for human or veterinary use.

CP21R7, also known CP21, is a potent and selective GSK-3β inhibitor.

Chemical Structure

CP21R7

CAS#125314-13-8

Theoretical Analysis

MedKoo Cat#: 530344

Name: CP21R7

CAS#: 125314-13-8

Chemical Formula: C19H15N3O2

Exact Mass: 317.1164

Molecular Weight: 317.35

Elemental Analysis: C, 71.91; H, 4.76; N, 13.24; O, 10.08

Price and Availability

Size	Price	Availability
5mg	USD 90.00	Ready to ship
10mg	USD 150.00	Ready to ship
25mg	USD 250.00	Ready to ship
50mg	USD 450.00	Ready to ship
100mg	USD 750.00	Ready to ship
200mg	USD 1,250.00	Ready to ship
500mg	USD 1,950.00	Ready to ship
1g	USD 2,950.00	Ready to ship

Bulk Inquiry

Buy Now

Add to Cart

Related CAS #

No Data

Synonym

CP21R7; CP21R7; CP21R7; CP21; CP-21; CP 21.

IUPAC/Chemical Name

3-(3-Aminophenyl)-4-(1-methyl-3-indolyl)-1H-pyrrole-2,5-dione

InChi Key

RGTAEYDIDMGJLX-UHFFFAOYSA-N

InChi Code

InChI=1S/C19H15N3O2/c1-22-10-14(13-7-2-3-8-15(13)22)17-16(18(23)21-19(17)24)11-5-4-6-12(20)9-11/h2-10H,20H2,1H3,(H,21,23,24)

SMILES Code

O=C(C(C1=CC=CC(N)=C1)=C2C3=CN(C)C4=C3C=CC=C4)NC2=O

Appearance

Orange solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Certificate of Analysis

View CoA: current batch, Lot#T7K08C17

QC Data

View QC data: current batch, Lot#T7K08C17

Safety Data Sheet (SDS)

View Safety Data Sheet (SDS)

Instruction

View handling instruction

Biological target:

CP21R7 is potent GSK-3β inhibitor, with an IC50 of 1.8 nM and also shows inhibitory activitiy against PKCα, with an IC50 of 1900 nM.

In vitro activity:

Further, the effect of Wnt pathway in the inhibition of migration after the silencing of BCL9, was ascertained by investigating whether the migration ability was restored in the presence of a Wnt signaling agonist. CP21, a highly selective inhibitor of GSK3β, was utilized to activate the Wnt signaling and explore the effect on migration in A549/DDP cell line after treatment with shRNA. The application of CP21 resulted in significant partial recovered the expression of β-catenin after knockdown of BCL9 compared (Fig. 4A). Furthermore, the migration distance and the number of cells that invaded into the lower chamber were significantly increased compared with the group that was not treated with the agonist (Fig. 4B and C), proving that CP21 could attenuate the negative regulation of BCL9 silencing to EMT. The ability of metastasis and invasion was partially restored after the treatment with CP21, which proved that the Wnt pathway plays a crucial role in reducing cell migration after BCL9 silencing in CR NSCLCs. Reference: Life Sci. 2018 Sep 1;208:284-294. https://pubmed.ncbi.nlm.nih.gov/30009824/

In vivo activity:

TBD

	Solvent	mg/mL	mM
Solubility
	DMSO	47.5	149.68

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 317.35 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Zhang Y, Zhang Q, Chen H, Wang C. BCL9 promotes epithelial mesenchymal transition and invasion in cisplatin resistant NSCLC cells via β-catenin pathway. Life Sci. 2018 Sep 1;208:284-294. doi: 10.1016/j.lfs.2018.07.023. Epub 2018 Jul 23. PMID: 30009824.

In vitro protocol:

In vivo protocol:

TBD