Synonym
Ro 14-7437; Ro 147437; Ro-14-7437.
IUPAC/Chemical Name
4H-Imidazo(1,5-a)(1,4)benzodiazepine-3-carboxylic acid, 5,6-dihydro-5-methyl-6-oxo-, ethyl ester
InChi Key
HNGRWUCKESPKRC-UHFFFAOYSA-N
InChi Code
InChI=1S/C15H15N3O3/c1-3-21-15(20)13-12-8-17(2)14(19)10-6-4-5-7-11(10)18(12)9-16-13/h4-7,9H,3,8H2,1-2H3
SMILES Code
O=C(C1=C(CN2C)N(C=N1)C3=CC=CC=C3C2=O)OCC
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
Preparing Stock Solutions
The following data is based on the
product
molecular weight
285.30
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
| 1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
| 5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
| 10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
| 50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
1: Harris BD, Moody EJ, Gu ZQ, Skolnick P. Contribution of "diazepam-insensitive" GABAA receptors to the alcohol antagonist properties of Ro 15-4513 and related imidazobenzodiazepines. Pharmacol Biochem Behav. 1995 Sep;52(1):113-8. PubMed PMID: 7501652.
2: Püspök A, Herneth A, Steindl P, Ferenci P. Hepatic encephalopathy in rats with thioacetamide-induced acute liver failure is not mediated by endogenous benzodiazepines. Gastroenterology. 1993 Sep;105(3):851-7. PubMed PMID: 8359654.
3: Basile AS, Gammal SH, Mullen KD, Jones EA, Skolnick P. Differential responsiveness of cerebellar Purkinje neurons to GABA and benzodiazepine receptor ligands in an animal model of hepatic encephalopathy. J Neurosci. 1988 Jul;8(7):2414-21. PubMed PMID: 2854841.
4: Laurent JP. [Pharmacologic bases of the action of benzodiazepine ligands]. Rev Med Suisse Romande. 1987 Sep;107(9):711-5. Review. French. PubMed PMID: 2827289.
5: Nistri A, Berti C. GABA-induced depolarizing responses of the frog spinal cord can be either enhanced or antagonized by the benzodiazepine midazolam and the methylxanthine caffeine. Neurosci Lett. 1984 Jun 29;47(3):277-81. PubMed PMID: 6089044.
6: Krespan B, Springfield SA, Haas H, Geller HM. Electrophysiological studies on benzodiazepine antagonists. Brain Res. 1984 Mar 19;295(2):265-74. PubMed PMID: 6424866.
7: Nistri A, Berti C. Potentiating action of midazolam on GABA-mediated responses and its antagonism by Ro 14-7437 in the frog spinal cord. Neurosci Lett. 1983 Aug 29;39(2):199-204. PubMed PMID: 6605499.
8: Carlen PL, Gurevich N, Polc P. The excitatory effects of the specific benzodiazepine antagonist Ro14-7437, measured intracellularly in hippocampal CA1 cells. Brain Res. 1983 Jul 18;271(1):115-9. PubMed PMID: 6883110.
