Maleimide-PEG4-NHS, also known as Mal-PEG4-NHS or NHS-PEG4-maleimide, is a sulfhydryl and amine reactive heterofuncational PEG linker. The chemical bonds formed through Maleimide-PEG4-NHS linker are stable and are not cleavable. The NHS ester reacts with amino groups at pH 7-9 to form stable amide bond while maleimide reacts with thiol groups at pH 6.5-7.5 to form stable thiol-ether bond. Maleimide-PEG4-NHS is a useful linker for various biomolecule crosslinking applications (such as to make antibody drug conjugates (ADCs).
MedKoo Cat#: 620107
Name: Maleimide-PEG4-NHS
CAS#: 1286754-10-6 (acid)
Chemical Formula: C18H24N2O10
Exact Mass: 428.1431
Molecular Weight: 428.39
Elemental Analysis: C, 50.47; H, 5.65; N, 6.54; O, 37.35
The following data is based on the product molecular weight 428.39 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
